Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,309,016 C→T *418* (TAG→TAA)  kch ← voltage‑gated potassium channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,309,0160CT100.0% 152.8 / NA 48*418* (TAG→TAA) kchvoltage‑gated potassium channel
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/25);  total (23/25)

ATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCA  >  NC_000913/1308890‑1309133
                                                                                                                              |                                                                                                                     
aTCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGcc                                                                                                           >  2:1010482/1‑139 (MQ=255)
                  tGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCt                                                                                         >  1:824039/1‑139 (MQ=255)
                      ttCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCg                                                                                     >  2:650622/1‑139 (MQ=255)
                                 caGCGTAACTCGTTAGCGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATCACGGTGACCAGTAAGTTTACTCTTCTGACTCTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGATTCTAGTTCa                                                                          >  2:999177/1‑139 (MQ=255)
                                                    ttGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAc                                                       >  1:820033/1‑139 (MQ=255)
                                                     tGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATAcc                                                      <  1:202326/139‑1 (MQ=255)
                                                             aCAATATCTCAATATACTGGGCATCATTGCCGTGCCCAATAAGTTTGCTCTTCCGTCTGTAAACTTTATTTTTGCGCCGATTCTTTATTGTCAGCTTGCGTTTCTAGCTCATCGTTATAGCTGAACAGACCATGACGTg                                              <  2:735055/139‑1 (MQ=255)
                                                                    cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTc                                                                                             <  2:465580/85‑1 (MQ=255)
                                                                    cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTc                                                                                             >  1:465580/1‑85 (MQ=255)
                                                                    cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                       >  1:124363/1‑139 (MQ=255)
                                                                    cTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaa                                       <  1:690743/139‑1 (MQ=255)
                                                                         tGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAacaaca                                  <  2:242110/139‑1 (MQ=255)
                                                                             cTGGGCACCATTGTGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                              >  2:48887/1‑139 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGtt                                                                                   <  1:587867/86‑1 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGtt                                                                                   <  2:909717/86‑1 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGtt                                                                                   >  2:587867/1‑86 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGtt                                                                                   >  1:909717/1‑86 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                >  2:90974/1‑105 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                <  1:90974/105‑1 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                              >  1:707222/1‑139 (MQ=255)
                                                                             cTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGa                              >  2:549302/1‑139 (MQ=255)
                                                                               gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                    >  1:498295/1‑115 (MQ=255)
                                                                               gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCAt                                                    <  2:498295/115‑1 (MQ=255)
                                                                               gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                               >  1:366155/1‑120 (MQ=255)
                                                                               gggCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCt                                               <  2:366155/120‑1 (MQ=255)
                                                                                     cATTGTGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCa                      <  1:48887/139‑1 (MQ=255)
                                                                                     cATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCa                      >  1:746024/1‑139 (MQ=255)
                                                                                        tGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                   <  1:549302/139‑1 (MQ=255)
                                                                                        tGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCatat                   <  2:824039/139‑1 (MQ=255)
                                                                                          cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCttt                                                                                       <  2:216899/69‑1 (MQ=255)
                                                                                          cGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCttt                                                                                       >  1:216899/1‑69 (MQ=255)
                                                                                            gTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCatt               >  1:575976/1‑139 (MQ=255)
                                                                                             tGCCCAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCatta              <  2:124363/139‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                      <  1:80777/65‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTc                                                                                      >  2:80777/1‑65 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                <  1:833808/87‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGc                                                                >  2:833808/1‑87 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                 >  2:221430/1‑102 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGAc                                                 <  1:221430/102‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt            <  2:820033/139‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt            <  2:575976/139‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCattatt            <  2:916443/139‑1 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTAAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAg                                                                              >  2:114100/1‑73 (MQ=255)
                                                                                               cccAATAAGTTTACTCTTCCGTCTTAAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAg                                                                              <  1:114100/73‑1 (MQ=255)
                                                                                                ccAATAAGTTTACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCAttatta           <  2:746024/139‑1 (MQ=255)
                                                                                                         ttACTCTTCCGTCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCa  <  2:707222/139‑1 (MQ=255)
                                                                                                                    tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                     <  1:543462/93‑1 (MQ=255)
                                                                                                                    tCTTTAAACTTTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTcaaca                                     >  2:543462/1‑93 (MQ=255)
                                                                                                                              |                                                                                                                     
ATCCACAGCATCAGGAAGTGCATTCTAAACAAACAGCGTAACTCGTTATTGTTTGCGATCTACAATATCTAAATGTACTGGGCACCATTGCGGTGCCCAATAAGTTTACTCTTCCGTCTTTAAACTCTATTTTTGCGCCGATTCTTTACTGTCAGCTTTCGTTTCTAGTTCATCGTTATCGCTGAAAATACCATGACCTGAGTTCAACAACATTGAAACGAGCATATCATTATTAATCTCTTCA  >  NC_000913/1308890‑1309133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑