Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,505	C→T	S5N (AGC→AAC)  *322* (TAG→TAA)	sapC ← sapB ←	antimicrobial peptide transport ABC transporter permease antimicrobial peptide transport ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,505	0	C	T	100.0%	123.9 / NA	39	S5N (AGC→AAC)  *322* (TAG→TAA)	sapC sapB	antimicrobial peptide transport ABC transporter permease antimicrobial peptide transport ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/19);  total (20/19)

CGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAT  >  NC_000913/1354369‑1354621                                                                                                                                         |                                                                                                                     cgcAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTa                                                                                                                    >  1:944727/1‑139 (MQ=255)         aGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAgg                                                                                                            <  2:399079/139‑1 (MQ=255)            cgccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCAt                                                                                                         <  1:910977/139‑1 (MQ=255)              ccAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATAc                                                                                                       <  2:461305/139‑1 (MQ=255)                              cagccagcCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                       <  1:610486/139‑1 (MQ=255)                              cagccagcCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATAGTAAGGCATACCATTCCTTATGTTTCa                                                                                       <  1:1020192/139‑1 (MQ=255)                               agccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                       <  2:51161/138‑1 (MQ=255)                               agccagccCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                       >  1:51161/1‑138 (MQ=255)                                      cccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTa                                                                               >  1:112355/1‑139 (MQ=255)                                      cccGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTa                                                                               >  2:76758/1‑139 (MQ=255)                                         gcgcAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGcc                                                                            >  1:976939/1‑139 (MQ=255)                                               ccGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCa                                                                      >  2:940928/1‑139 (MQ=255)                                                     aGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCaaa                                                                <  1:940928/139‑1 (MQ=255)                                                       gCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAat                                                              >  1:427967/1‑139 (MQ=255)                                                                cGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAAt                                                     <  1:895749/139‑1 (MQ=255)                                                                      ggCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGtt                                               <  1:76758/139‑1 (MQ=255)                                                                          tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACa                                           >  2:514825/1‑139 (MQ=255)                                                                          tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACa                                           >  2:91821/1‑139 (MQ=255)                                                                          tCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACa                                           >  1:1068232/1‑139 (MQ=255)                                                                             cTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACaata                                        <  2:1068232/139‑1 (MQ=255)                                                                                  aaaTTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGAc                                   <  2:944727/139‑1 (MQ=255)                                                                                        gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAggg                                                                                  <  1:51118/86‑1 (MQ=255)                                                                                        gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAggg                                                                                  >  2:51118/1‑86 (MQ=255)                                                                                        gcgcCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGa                             >  2:142196/1‑139 (MQ=255)                                                                                              ggCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCcaca                       <  2:427967/139‑1 (MQ=255)                                                                                                   tACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCa                  >  1:297500/1‑139 (MQ=255)                                                                                                      gCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCa                                                                                                          >  1:457281/1‑48 (MQ=255)                                                                                                      gCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCa                                                                                                          <  2:457281/48‑1 (MQ=255)                                                                                                         gCGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTAAGCCACACACCATCACTc            >  1:1010/1‑139 (MQ=255)                                                                                                          cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                 >  2:990801/1‑53 (MQ=255)                                                                                                          cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTcc                                                                                                 <  1:990801/53‑1 (MQ=255)                                                                                                          cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                       <  1:933450/63‑1 (MQ=255)                                                                                                          cGTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCa                                                                                       >  2:933450/1‑63 (MQ=255)                                                                                                           gTGCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcg          <  1:91821/139‑1 (MQ=255)                                                                                                             gCCCGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcggc        >  1:58533/1‑139 (MQ=255)                                                                                                              cccGGCGGGCGCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCcggcg       >  1:510020/1‑139 (MQ=255)                                                                                                                   cgggcgCTTTTCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAt  <  1:142196/139‑1 (MQ=255)                                                                                                                            ttCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTTGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaaa   <  2:770061/129‑1 (MQ=255)                                                                                                                            ttCGCTGTATACGTTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGaaa   >  1:770061/1‑129 (MQ=255)                                                                                                                                         |                                                                                                                     CGCAAACCAGCCGCCAAAAATACACAGTACAGCCAGCCCCGCGCAGCCGTACAGGCCGACCATCGCAGAGGCGTCACTATAAAATTTGCGCCAGGCGGTACGCAGCGTGCCCGGCGGGCGCTTTTCGCTGTATACGCTATCGTAAGGCATACCATTCCTTATGTTTCAGAGGGTTAGCCATGGCACCCAAAATATCAGAAATCACGTTAACAATAATGACCAGTGAGCCACACACCATCACTCCGGCGGAAAT  >  NC_000913/1354369‑1354621

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑