Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	117,798	A→G	L283P (CTA→CCA)	nadC ←	quinolinate phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	117,798	0	A	G	100.0%	109.8 / NA	31	L283P (CTA→CCA)	nadC	quinolinate phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/16);  total (15/16)

GAAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTG  >  NC_000913/117663‑117927                                                                                                                                        |                                                                                                                                  gAAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGgcg                                                                                                                                <  2:804003/139‑1 (MQ=255)   aaGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                              <  1:54844/139‑1 (MQ=255)   aaGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                              <  1:893514/139‑1 (MQ=255)       ctaGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGa                                                                                                                          >  1:566447/3‑139 (MQ=255)                      gCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                              >  2:560232/1‑120 (MQ=255)                      gCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCa                                                                                                                              <  1:560232/120‑1 (MQ=255)                                         ggAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTc                                                                                        >  2:1054493/1‑139 (MQ=255)                                             ggggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                    <  1:1054493/139‑1 (MQ=255)                                               ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCa                                                                                   >  1:434543/1‑138 (MQ=255)                                               ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCa                                                                                   <  2:434543/138‑1 (MQ=255)                                               ggAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAg                                                                                  >  1:53297/1‑139 (MQ=255)                                                          gcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAg                                                                                  <  1:93485/128‑1 (MQ=255)                                                          gcccgcGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAg                                                                                  >  2:93485/1‑128 (MQ=255)                                                                        ggATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGAACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGac                                                         <  2:1075881/139‑1 (MQ=255)                                                                               tCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                    <  1:613982/105‑1 (MQ=255)                                                                               tCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                    >  2:613982/1‑105 (MQ=255)                                                                                          ttAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGa                                                          >  1:913375/1‑120 (MQ=255)                                                                                          ttAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGa                                                          <  2:913375/120‑1 (MQ=255)                                                                                           tAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGc                                      >  2:1054799/1‑139 (MQ=255)                                                                                             gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                    >  1:90494/1‑91 (MQ=255)                                                                                             gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGc                                                                                    <  2:90494/91‑1 (MQ=255)                                                                                             gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                    <  2:281724/107‑1 (MQ=255)                                                                                             gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGa                                                                    >  1:281724/1‑107 (MQ=255)                                                                                             gCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCg                                    >  1:969056/1‑139 (MQ=255)                                                                                                aaaaCGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTg                                 <  2:53297/139‑1 (MQ=255)                                                                                                          gAAAGGTCGAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGa                       <  1:905279/139‑1 (MQ=255)                                                                                                                  gAGTGCTTGTACGTGTTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTcg               <  2:566447/139‑1 (MQ=255)                                                                                                                               tgtTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAtctgttctg  >  1:1006091/1‑139 (MQ=255)                                                                                                                               tgtTTAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCAtctgttctg  >  1:575933/1‑139 (MQ=255)                                                                                                                                 tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                     <  1:352015/54‑1 (MQ=255)                                                                                                                                 tttAGTTGGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACg                                                                                     >  2:352015/1‑54 (MQ=255)                                                                                                                                    agttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACt                                                      <  2:1003811/82‑1 (MQ=255)                                                                                                                                    agttggCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACt                                                      >  1:1003811/1‑82 (MQ=255)                                                                                                                                        |                                                                                                                                  GAAAGACAAGAAATTTGCGAGGCGTTACGAAGAAAGTTGGGGAAGGGGAGATTATCCGCCCGCGATGGAGCGGATAAATCTGTCAACTATTAGCGAAAACGCATTGAAAGGTCGAGTGCTTGTACGTGTTTAGTTAGCGCACCGACGGAGATAAAGTCCACGCCCGTTTCGGCAAATTCACGCAGTGTTTTGTCAGTGACGTTGCCAGACACTTCCAGTAGCGCCTTGCCGTTGGTGCGTTTGACGGCTTCGCGCATCTGTTCTG  >  NC_000913/117663‑117927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑