| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 122,856 | G→A | *255* (TAG→TAA) | pdhR → | pyruvate dehydrogenase complex repressor, autorepressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 122,856 | 0 | G | A | 97.4% | 115.5 / ‑6.3 | 38 | *255* (TAG→TAA) | pdhR | pyruvate dehydrogenase complex repressor, autorepressor |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (18/19); total (18/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAA > NC_000913/122727‑122989 | tAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTc > 2:765336/1‑139 (MQ=255) cgATGCATCGCATCGCCATCTGGCCATTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCaga < 1:693622/139‑1 (MQ=255) cgAAGCATCGCTTCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCaga < 1:695923/139‑1 (MQ=255) cgAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCaga > 1:946581/1‑139 (MQ=255) tcgcatcgcTATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGAtgtt < 1:30162/139‑1 (MQ=255) ccATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTaaaaa < 1:36475/115‑1 (MQ=255) ccATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTaaaaa > 2:36475/1‑115 (MQ=255) tttATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCAtata > 2:533367/1‑139 (MQ=255) gaagaaATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAgcg < 1:227382/139‑1 (MQ=255) cAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTc > 1:307785/1‑73 (MQ=255) cAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTc < 2:307785/73‑1 (MQ=255) gAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAAc < 2:534511/139‑1 (MQ=255) tCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGt < 2:940101/139‑1 (MQ=255) agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGa > 2:750188/1‑123 (MQ=255) agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGa < 1:750188/123‑1 (MQ=255) agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGc > 2:429459/1‑139 (MQ=255) agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGc > 2:197749/1‑139 (MQ=255) agCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGc > 1:1044274/1‑139 (MQ=255) ccgccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATc < 2:508557/69‑1 (MQ=255) ccgccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATc > 1:508557/1‑69 (MQ=255) ccgccgTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCtt > 2:728013/1‑139 (MQ=255) aGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCga > 1:282577/1‑139 (MQ=255) tctcTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTa < 1:704466/100‑1 (MQ=255) tctcTGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTa > 2:704466/1‑100 (MQ=255) tctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCa < 1:533367/139‑1 (MQ=255) ctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAAc > 2:1060164/1‑107 (MQ=255) ctGCGTCGTCTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAAc < 1:1060164/107‑1 (MQ=255) gtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc < 1:918247/68‑1 (MQ=255) gtcTGGAGCAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAgc > 2:918247/1‑68 (MQ=255) cAACGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAg < 1:58218/139‑1 (MQ=255) cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGtt > 2:99809/1‑118 (MQ=255) cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGtt < 1:99809/118‑1 (MQ=255) cGAAAGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGaaa > 1:902291/1‑139 (MQ=255) aGAATTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGTGAGAGTTCAATGGGACAGGTTCCAGAAAACTc > 2:159891/1‑139 (MQ=255) aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGTGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa < 1:159891/139‑1 (MQ=255) aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa < 2:839891/139‑1 (MQ=255) aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa < 2:902291/139‑1 (MQ=255) aaTTAATGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCaa < 1:521568/139‑1 (MQ=255) | TAAGCCGGAAGAAGCGCGCGAAGCATCGCATCGCCATCTGGCCTTTATCGAAGAAATTTTGCTCGACAGAAGTCGTGAAGAGAGCCGCCGTGAGCGTTCTCTGCGTCGTCTGGAGCAACGAAAGAATTAGTGATTTTTCTGGTAAAAATTATCCAGAAGATGTTGTAAATCAAGCGCATATAAAAGCGCGGCAACTAAACGTAGAACCTGTCTTATTGAGCTTTCCGGCGAGAGTTCAATGGGACAGGTTCCAGAAAACTCAA > NC_000913/122727‑122989 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |