Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,419,344	G→A	A38V (GCT→GTT)	ydaG ←	Rac prophage, uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,419,344	0	G	A	100.0%	116.8 / NA	38	A38V (GCT→GTT)	ydaG	Rac prophage, uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/22);  total (16/22)

TATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGG  >  NC_000913/1419224‑1419479                                                                                                                         |                                                                                                                                        tataCGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCaga                                                                                                                       <  1:514171/139‑1 (MQ=255)           ggCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTc                                                                                                             >  2:780580/1‑139 (MQ=255)                tGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                        <  2:152040/139‑1 (MQ=255)                tGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                        >  1:991440/1‑139 (MQ=255)                tGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                        >  2:891466/1‑139 (MQ=255)                  aaCGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgctg                                                                                                      >  2:845341/1‑139 (MQ=255)                     gTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGt                                                                                                   <  2:975774/139‑1 (MQ=255)                      ttCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtg                                                                                                         <  1:635722/132‑1 (MQ=255)                      ttCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtg                                                                                                         >  2:635722/1‑132 (MQ=255)                               ggTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGca                                                                                         <  2:197884/139‑1 (MQ=255)                               ggTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGca                                                                                         <  2:1067947/139‑1 (MQ=255)                                 ttGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGcaca                                                                                       >  2:41819/1‑139 (MQ=255)                                   ggggAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGc                                                                                     <  1:41819/139‑1 (MQ=255)                                     tgAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCt                                                                                   <  1:479141/138‑1 (MQ=255)                                       aaCACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTgg                                                                                 <  1:891466/139‑1 (MQ=255)                                          acGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTg                                                                              >  1:153283/1‑139 (MQ=255)                                                    ccAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATAc                                                                    <  1:955105/139‑1 (MQ=255)                                                        ggATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGca                                                                >  2:848320/1‑139 (MQ=255)                                                             cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGTCTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                           <  2:402645/139‑1 (MQ=255)                                                             cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                           >  1:860282/1‑139 (MQ=255)                                                             cGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAAt                                                           <  1:848320/139‑1 (MQ=255)                                                               ttgttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                        >  2:139891/1‑92 (MQ=255)                                                               ttgttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTtgc                                                                                                        <  1:139891/92‑1 (MQ=255)                                                               ttgttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAAAAATcc                                                         >  2:1063087/1‑139 (MQ=255)                                                                  ttgCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATc                                                      <  2:1038234/139‑1 (MQ=255)                                                                     cctaGGTCGATTTTTTTCATTTTTCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTACCAGAGCTCCCAGTCGTTTCTGCCTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCaaa                                                   <  2:153283/136‑1 (MQ=255)                                                                      cGAGGTCGATTTTTTTCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAAt                                                  <  2:1068778/139‑1 (MQ=255)                                                                            caaTTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAGATACTGaa                                            <  2:995546/137‑1 (MQ=255)                                                                            cGATTTTTTTCATTTTGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGaa                                            <  1:750404/139‑1 (MQ=255)                                                                                         tttGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATg                               >  2:1051986/1‑139 (MQ=255)                                                                                          ttGCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGc                              >  1:252610/1‑139 (MQ=255)                                                                                            gCGAATCTCACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCAc                            <  2:860282/139‑1 (MQ=255)                                                                                                 tctcACATCTTGTTGCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCt                       <  1:780580/139‑1 (MQ=255)                                                                                                     acaTCTTGTTGCTACGTATAACGCCTTCTGCCTGCCAGAGATCCCAGTAGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTGCGTAATGCACCATCTTAtc                   >  1:78387/1‑139 (MQ=255)                                                                                                        tCTTGTTGCTACGTATAACGACTTCTGCTTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTAtctct                <  1:1045411/139‑1 (MQ=255)                                                                                                             ttgCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCt           >  2:120350/1‑139 (MQ=255)                                                                                                               gCTACGTATAACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTa         >  1:205550/1‑139 (MQ=255)                                                                                                                      ataACGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCgg  <  2:252610/139‑1 (MQ=255)                                                                                                                         |                                                                                                                                        TATACGTCATGGCAGTGAACGTTCCATCCTGGTTGGGGAACACGCCGCACACCAGGGATTCGTTGTTGCCGAGGTCGATTTTTTGCATTTTGCGAATCTCACATCTTGTTGCTACGTATAGCGACTTCTGCCTGCCAGAGATCCCAGTCGTTGCTGCGTAAAGCCTGCACAGCCTGGTTGTAAGTGATACCGCAACAATCCATCAAATACTGAACTACTTCGTAATGCACCATCTTATCTCTCCCCTTAACGCCGG  >  NC_000913/1419224‑1419479

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑