Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,639,030	C→T	*166* (TAG→TAA)	rzpQ ←	Rz‑like protein, Qin prophage

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,639,030	0	C	T	100.0%	67.3 / NA	22	*166* (TAG→TAA)	rzpQ	Rz‑like protein, Qin prophage
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/11);  total (11/11)

GTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCATT  >  NC_000913/1638908‑1639165                                                                                                                           |                                                                                                                                        gtgtCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCtttt                                                                                                                         <  2:949202/139‑1 (MQ=255)                  aCCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTa                                                                                                                <  1:397805/131‑1 (MQ=255)                  aCCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTa                                                                                                                >  2:397805/1‑131 (MQ=255)                      tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGa                                                                                                    >  2:653809/1‑139 (MQ=255)                              aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGa                                                                                            >  1:412857/1‑139 (MQ=255)                               cccTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                           >  1:971931/1‑139 (MQ=255)                                    aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTa                                                                                                                       >  2:689960/1‑106 (MQ=255)                                    aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTa                                                                                                                       <  1:689960/106‑1 (MQ=255)                                          tCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTc                                                                                  >  1:553492/1‑137 (MQ=255)                                          tCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTc                                                                                  <  2:553492/137‑1 (MQ=255)                                           cAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTg                                                                               <  2:412857/139‑1 (MQ=255)                                                                    ccTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTc                                                      >  1:298276/1‑139 (MQ=255)                                                                        tttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCa                                                  <  1:653809/139‑1 (MQ=255)                                                                         ttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                                 <  2:799741/139‑1 (MQ=255)                                                                                    ttACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCt                                      <  2:298276/139‑1 (MQ=255)                                                                                             gcgATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATa                                                                                                      <  1:814496/66‑1 (MQ=255)                                                                                             gcgATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATa                                                                                                      >  2:814496/1‑66 (MQ=255)                                                                                             gcgATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCa                             <  2:971931/139‑1 (MQ=255)                                                                                                           ccGATTTAATATTAGCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCGTGCGATGTAGCGTTCGTCAGCAATTTGAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCAt               >  1:652158/1‑139 (MQ=255)                                                                                                           ccGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCAt               >  2:154031/1‑139 (MQ=255)                                                                                                                 tAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGt         <  1:154031/139‑1 (MQ=255)                                                                                                                        aCCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCAtt  >  1:437178/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                        GTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCATT  >  NC_000913/1638908‑1639165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑