| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,831,911 | T→C | I24M (ATA→ATG) | astC ← | succinylornithine transaminase, PLP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,831,911 | 0 | T | C | 93.0% | 132.9 / ‑0.1 | 43 | I24M (ATA→ATG) | astC | succinylornithine transaminase, PLP‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (14/26); total (15/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
ACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAAT > NC_000913/1831776‑1832026 | aCTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCATGTACCGGcata > 1:347876/1‑139 (MQ=255) ttcgttcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGcc < 1:204389/139‑1 (MQ=255) gttcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGa < 1:201237/139‑1 (MQ=255) tcAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGc < 2:564097/139‑1 (MQ=255) gcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTaaa < 2:241606/139‑1 (MQ=255) gcTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTaaa < 2:653383/139‑1 (MQ=255) cTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAAc < 1:1054955/139‑1 (MQ=255) gcgcGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCATGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCAcg < 2:347876/139‑1 (MQ=255) gcgcGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCAcg > 1:568594/1‑139 (MQ=255) gcgcGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCAcg > 2:223592/1‑139 (MQ=255) cGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGt > 1:425689/1‑103 (MQ=255) cGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGt < 2:425689/103‑1 (MQ=255) gCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCt < 2:1069051/139‑1 (MQ=255) gCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCt < 1:966501/139‑1 (MQ=255) tGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGt > 2:926016/1‑127 (MQ=255) tGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGt < 1:926016/127‑1 (MQ=255) ccGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGc < 1:223592/139‑1 (MQ=255) aaGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACct < 1:803507/139‑1 (MQ=255) ctctTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAaat < 2:568594/139‑1 (MQ=255) tttCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAaataat < 1:840585/139‑1 (MQ=255) tCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGc > 2:144309/1‑118 (MQ=255) tCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGc < 1:144309/118‑1 (MQ=255) ccccTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCg < 1:558565/139‑1 (MQ=255) tgctgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGttat > 2:799925/1‑139 (MQ=255) gctgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGt > 1:113344/1‑65 (MQ=255) gctgATCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGt < 2:113344/65‑1 (MQ=255) aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc < 2:94162/111‑1 (MQ=255) aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc > 1:94162/1‑111 (MQ=255) aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCg > 1:14248/1‑130 (MQ=255) aTCCCACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCg < 2:14248/130‑1 (MQ=255) aTCCCACAAGCCCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCAGAGCGTAAACAGGTATCATCCTTTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTtata < 2:402681/139‑1 (MQ=255) cccACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc > 2:397108/1‑109 (MQ=255) cccACAAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc < 1:397108/109‑1 (MQ=255) cacaAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc < 2:192322/107‑1 (MQ=255) cacaAGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc > 1:192322/1‑107 (MQ=255) aaGCGCGAACCTTCGCTACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaat > 1:914487/1‑139 (MQ=255) aaGCGCGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaat < 1:799925/139‑1 (MQ=255) tcgcgAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaataa < 2:839853/138‑1 (MQ=255) gcgcGAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaataa < 1:469380/139‑1 (MQ=255) cgcgAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATa < 1:937931/96‑1 (MQ=255) cgcgAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATa > 2:937931/1‑96 (MQ=255) cgcgAACCTTCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTtaataat > 2:926683/1‑139 (MQ=255) tCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc < 2:351761/92‑1 (MQ=255) tCGCCACGTACCGGCATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGAc > 1:351761/1‑92 (MQ=255) | ACTCGCCTGTTCGTTCAGCGCTTCACGCAGTTCCGGATGCGCATGGCCCAGCGCGTTCACCGCAATGCCACCCGCGAAGTCGATATACTCTTTCCCCTGCTGATCCCACAAGCGCGAACCTTCGCCACGTACCGGTATAAAGGGTGCCGGAGCGTAAACAGGTATCATCCATTCATCAAAGTTTTCACGCGTAATTGGCTGAGACATAGCGACCTCTACAGTAAATAATTCGTTATTTATATGTTAATAAT > NC_000913/1831776‑1832026 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |