Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,930,941	G→A	G21S (GGT→AGT)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,930,941	0	G	A	100.0%	227.2 / NA	70	G21S (GGT→AGT)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (39/31);  total (39/31)

ATCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAT  >  NC_000913/1930811‑1931075                                                                                                                                   |                                                                                                                                       aTCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTg                                                                                                                                >  1:569243/1‑139 (MQ=255) aTCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTg                                                                                                                                >  2:255126/1‑139 (MQ=255)                   cGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATc                                                                                                              >  2:917012/1‑139 (MQ=255)                            tttCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAg                                                                                                     <  1:917012/139‑1 (MQ=255)                                          cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   >  2:988528/1‑95 (MQ=255)                                          cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   <  1:988528/95‑1 (MQ=255)                                          cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACt                                                                                                                                 >  1:430519/1‑97 (MQ=255)                                          cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACt                                                                                                                                 <  2:430519/97‑1 (MQ=255)                                          cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCa                                                                                                      <  1:536862/124‑1 (MQ=255)                                          cgcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCa                                                                                                      >  2:536862/1‑124 (MQ=255)                                           gcgcgGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTagag                                                                                      >  2:58869/1‑139 (MQ=255)                                                     aaTCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCg                                                                            <  1:28872/139‑1 (MQ=255)                                                           gggATATTCGTTATGACGTTATTAGGCACTGCGCTGCTTCCGGCAGCAACTCGCGTGCTGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATc                                                                      >  2:225003/1‑139 (MQ=255)                                                              atatTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAg                                                                                                     <  2:954078/105‑1 (MQ=255)                                                              atatTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAg                                                                                                     >  1:954078/1‑105 (MQ=255)                                                                            gTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCa                                                     <  2:982100/139‑1 (MQ=255)                                                                            gTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCa                                                     >  1:982100/1‑139 (MQ=255)                                                                              tattaGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGc                                                   >  2:773421/1‑139 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   <  1:738210/49‑1 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGaa                                                                                                                                   >  2:738210/1‑49 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTa                                                                                                                            <  1:29573/56‑1 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTa                                                                                                                            >  2:29573/1‑56 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTg                                                                                                                    >  2:40850/1‑64 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTg                                                                                                                    <  1:40850/64‑1 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTc                                         >  1:899671/1‑139 (MQ=255)                                                                                        tgcgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTc                                         >  1:488826/1‑139 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAAt                                                                                                               <  1:706097/67‑1 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAAt                                                                                                               <  1:759100/67‑1 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAAt                                                                                                               >  2:706097/1‑67 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  2:814828/1‑139 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  1:1072959/1‑139 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  2:639791/1‑139 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  2:1017455/1‑139 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgc                                       >  1:842719/1‑139 (MQ=255)                                                                                          cgctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTAAACTGGGTAAAGAAGTGGCAAt                                                                                                               >  2:759100/1‑67 (MQ=255)                                                                                           gctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTcgcg                                      <  1:814828/139‑1 (MQ=255)                                                                                            ctgcgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCgcgc                                     >  2:873056/1‑139 (MQ=255)                                                                                               cgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCg                                                                                                   <  2:116842/74‑1 (MQ=255)                                                                                               cgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCg                                                                                                   >  1:116842/1‑74 (MQ=255)                                                                                               cgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCg                                                                         >  1:429067/1‑100 (MQ=255)                                                                                               cgTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCg                                                                         <  2:429067/100‑1 (MQ=255)                                                                                                    ggCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCAc                                                       <  2:649608/113‑1 (MQ=255)                                                                                                    ggCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCAc                                                       >  1:649608/1‑113 (MQ=255)                                                                                                    ggCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTcc                             >  1:34660/1‑139 (MQ=255)                                                                                                    ggCAGCAACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCACATCGCTcc                             >  1:471445/1‑139 (MQ=255)                                                                                                     gcagcaACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTccc                            <  2:242009/139‑1 (MQ=255)                                                                                                      cagcaACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCa                           <  1:1017455/139‑1 (MQ=255)                                                                                                       agcaACTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCAt                          <  1:799921/139‑1 (MQ=255)                                                                                                          aaCTCGCGTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTc                       <  1:873056/139‑1 (MQ=255)                                                                                                              cgcgTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTa                   >  2:936055/1‑139 (MQ=255)                                                                                                                cgTGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAat                 <  1:58869/139‑1 (MQ=255)                                                                                                                  tGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCAt                                   <  2:417753/119‑1 (MQ=255)                                                                                                                  tGATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCAt                                   >  1:417753/1‑119 (MQ=255)                                                                                                                   gATGTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCACATCGCTCCCATGTCATTAATATg              <  2:471445/139‑1 (MQ=255)                                                                                                                      gTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCa                                                     >  2:1008054/1‑97 (MQ=255)                                                                                                                      gTTATTAGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCa                                                     <  1:1008054/97‑1 (MQ=255)                                                                                                                        tattaGGCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGa         <  2:34660/139‑1 (MQ=255)                                                                                                                             ggCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTg    <  1:936055/139‑1 (MQ=255)                                                                                                                             ggCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTg    <  2:488826/139‑1 (MQ=255)                                                                                                                             ggCTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTg    <  1:639791/139‑1 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGtct                                                                                                >  1:347979/1‑45 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGtct                                                                                                <  2:347979/45‑1 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCg                                                                                           >  1:868269/1‑50 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCg                                                                                           <  2:868269/50‑1 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  2:991860/1‑139 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  2:916397/1‑139 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  2:143952/1‑139 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  2:360493/1‑139 (MQ=255)                                                                                                                               cTCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAt  >  2:36186/1‑139 (MQ=255)                                                                                                                                tCCAGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCa                           <  1:667014/113‑1 (MQ=255)                                                                                                                                   |                                                                                                                                       ATCAGCAATAAAGACACACGCAAACGTTTTCGTTTATACTGCGCGCGGAATTAATCAGGGGATATTCGTTATGACGTTATTAGGCACTGCGCTGCGTCCGGCAGCAACTCGCGTGATGTTATTAGGCTCCGGTGAACTGGGTAAAGAAGTGGCAATCGAGTGTCAGCGTCTCGGCGTAGAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGAT  >  NC_000913/1930811‑1931075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑