Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,939,673	T→C	N174S (AAC→AGC)	lpxM ←	myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,939,673	0	T	C	100.0%	181.4 / NA	50	N174S (AAC→AGC)	lpxM	myristoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/26);  total (24/26)

ATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTA  >  NC_000913/1939539‑1939807                                                                                                                                       |                                                                                                                                       aTTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCa                                                                                                                                    <  1:58726/139‑1 (MQ=255)                     tGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGt                                                                                                                <  2:346062/139‑1 (MQ=255)                         ccTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  2:450679/139‑1 (MQ=255)                          cTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGccc                                                                                                           <  1:630521/139‑1 (MQ=255)                                gggTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                                             <  1:893061/131‑1 (MQ=255)                                gggTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGc                                                                                                             >  2:893061/1‑131 (MQ=255)                                 ggTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTAt                                                                                                    >  2:500991/1‑139 (MQ=255)                                  gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCGAAAGCGACGACGCACCGTGCTCCAGACATAATc                                                                                                                           >  2:634115/1‑115 (MQ=255)                                  gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATc                                                                                                                           >  2:312783/1‑115 (MQ=255)                                  gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATc                                                                                                                           <  1:634115/115‑1 (MQ=255)                                  gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATc                                                                                                                           <  1:312783/115‑1 (MQ=255)                                  gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGccc                                                                                                           <  1:589860/131‑1 (MQ=255)                                  gTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGccc                                                                                                           >  2:589860/1‑131 (MQ=255)                                   tAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATgg                                                                                                  >  1:487659/1‑139 (MQ=255)                                            tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                                         >  1:492732/1‑139 (MQ=255)                                            tCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCt                                                                                         >  1:754245/1‑139 (MQ=255)                                                cAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCa                                                                                                                                    <  1:639706/92‑1 (MQ=255)                                                cAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCa                                                                                                                                    >  2:639706/1‑92 (MQ=255)                                                 aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                                          >  2:689811/1‑117 (MQ=255)                                                 aGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCt                                                                                                          <  1:689811/117‑1 (MQ=255)                                                       ccTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTg                                                                              <  1:1073847/139‑1 (MQ=255)                                                        cTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  1:326339/108‑1 (MQ=255)                                                        cTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  2:326339/1‑108 (MQ=255)                                                                ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACAt                                                                                             <  2:617027/115‑1 (MQ=255)                                                                ccGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACAt                                                                                             >  1:617027/1‑115 (MQ=255)                                                                 cGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAg                                                                    <  1:500991/139‑1 (MQ=255)                                                                   aCTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  2:967810/1‑97 (MQ=255)                                                                   aCTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  1:967810/97‑1 (MQ=255)                                                                          gAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGc                                                           <  2:904680/139‑1 (MQ=255)                                                                             tGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATg                                                        >  2:576294/1‑139 (MQ=255)                                                                                 ttAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAg                                                    >  1:666053/1‑139 (MQ=255)                                                                                         gTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCg                                            >  1:642969/1‑139 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCGCCGTGCTCCa                                                                                                                                    <  1:48679/50‑1 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCGCCGTGCTCCa                                                                                                                                    >  2:48679/1‑50 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACa                                                                                                                                >  1:891223/1‑54 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACa                                                                                                                                <  2:891223/54‑1 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            <  1:298200/74‑1 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGcc                                                                                                            >  2:298200/1‑74 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                           >  1:853461/1‑139 (MQ=255)                                                                                          tCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGa                                           <  1:535677/139‑1 (MQ=255)                                                                                                          gacgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCgg                           <  2:853461/139‑1 (MQ=255)                                                                                                           acgacCGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGc                          >  2:111062/1‑139 (MQ=255)                                                                                                                cGCCAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAg                     <  2:754245/139‑1 (MQ=255)                                                                                                                  ccAAAGCGACGACGCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGaa                   >  1:708453/1‑139 (MQ=255)                                                                                                                          acgacgCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                                 >  1:6351/1‑37 (MQ=255)                                                                                                                          acgacgCACCGTGCTCCAGACATAATCAAAAACCggg                                                                                                                 <  2:6351/37‑1 (MQ=255)                                                                                                                          acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGc                                                                                       <  2:891741/63‑1 (MQ=255)                                                                                                                          acgacgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGc                                                                                       >  1:891741/1‑63 (MQ=255)                                                                                                                              cgCACCGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTtct       <  1:576294/139‑1 (MQ=255)                                                                                                                                   cGTGCTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGtta  <  2:383315/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ATTCGCTGTGCTCTGGGCCATGATCCTGATCGGGTAAATAATATCCCCAGTACCCCTGACGTACCGACTGGATGAATGGTTTAATACCGTCATTTCTCGCATGCAGACGACCGCCAAAGCGACGACGCACCGTGTTCCAGACATAATCAAAAACCGGGTTGCCCTGATTATGGAACATCGCTGCCATTTTCTGCCCTTGCGAGGCCATCAGCATGGCAGGAATATCGACGGCCCAACCGTGCGGCACCAGAAAGATAACTTTCTCGTTA  >  NC_000913/1939539‑1939807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑