Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,952,661	G→A	*132* (TAG→TAA)	yecN →	MAPEG family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,952,661	0	G	A	100.0%	152.8 / NA	48	*132* (TAG→TAA)	yecN	MAPEG family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/22);  total (26/22)

CATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGAT  >  NC_000913/1952527‑1952791                                                                                                                                       |                                                                                                                                   cATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACATTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTGTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGca                                                                                                                                <  1:719323/139‑1 (MQ=255)            ttttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCAc                                                                                                                     >  1:139071/1‑139 (MQ=255)             tttCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCAct                                                                                                                    >  2:929440/1‑139 (MQ=255)                catcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCActttc                                                                                                                 >  2:205996/1‑139 (MQ=255)                catcaCCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCActttc                                                                                                                 >  1:786858/1‑139 (MQ=255)                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTccc                                                                                                         >  2:452003/1‑139 (MQ=255)                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTccc                                                                                                         >  1:230875/1‑139 (MQ=255)                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTcc                                                                                                          <  2:454099/138‑1 (MQ=255)                        tCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTcc                                                                                                          >  1:454099/1‑138 (MQ=255)                          tGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGGTTTGGTTTTCTCCCTGGGTAAACGCAAAATACGCACCTTTCTTTTTCCCgg                                                                                                       >  2:278989/1‑139 (MQ=255)                          tGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCgg                                                                                                       >  2:884207/1‑139 (MQ=255)                           gTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                      <  2:786858/139‑1 (MQ=255)                           gTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGa                                                                                                      <  2:932513/139‑1 (MQ=255)                            ttCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGAt                                                                                                     >  1:250634/1‑139 (MQ=255)                               cGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCGTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCATAATACGCCACTTTCTTTTTCCCGGAtttt                                                                                                  <  2:340670/139‑1 (MQ=255)                                    gCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGt                                                                                             <  1:884207/139‑1 (MQ=255)                                                  tGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcgcg                                                                               <  1:205996/139‑1 (MQ=255)                                                                     gcTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                            <  2:139071/139‑1 (MQ=255)                                                                      cTGTTGGTTATTGTGCTTGCCAATCTTTTGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCt                                                           <  1:278989/139‑1 (MQ=255)                                                                        gTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTAt                                                         >  2:136132/1‑139 (MQ=255)                                                                                 ggTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACt                                                >  2:630229/1‑139 (MQ=255)                                                                                   tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                            <  1:558862/93‑1 (MQ=255)                                                                                   tGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGtt                                                                                            >  2:558862/1‑93 (MQ=255)                                                                                         cGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACt                                        >  1:519166/1‑139 (MQ=255)                                                                                                    tataTGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTtgat                             <  2:974286/139‑1 (MQ=255)                                                                                                         gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACg                        >  1:524893/1‑139 (MQ=255)                                                                                                         gCCCTGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACg                        >  2:94154/1‑139 (MQ=255)                                                                                                             tGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACg                                                                                                                         <  2:837211/38‑1 (MQ=255)                                                                                                             tGGGAGTTGGTTTTCTCCCTGCGTTAACGCACAATACg                                                                                                                         >  1:837211/1‑38 (MQ=255)                                                                                                              gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcg                                                                                 >  2:855866/1‑77 (MQ=255)                                                                                                              gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACcg                                                                                 <  1:855866/77‑1 (MQ=255)                                                                                                              gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                          >  2:414511/1‑132 (MQ=255)                                                                                                              gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGaa                          <  1:414511/132‑1 (MQ=255)                                                                                                              gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                   >  1:1076400/1‑139 (MQ=255)                                                                                                              gggAGTTGGTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAg                   >  2:113025/1‑139 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                             >  1:76554/1‑74 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGac                                                                             <  2:76554/74‑1 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                            <  2:187589/91‑1 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGcccc                                                            >  1:187589/1‑91 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt            >  2:448740/1‑139 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt            >  2:297147/1‑139 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt            >  2:857707/1‑139 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGt            <  1:630229/139‑1 (MQ=255)                                                                                                                     ggTTTTCTCCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGAGGAACGGGTAGCTGAAGt            <  2:82030/139‑1 (MQ=255)                                                                                                                          tctcCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTcc       <  2:230875/139‑1 (MQ=255)                                                                                                                           ctcCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc      <  1:857707/139‑1 (MQ=255)                                                                                                                           ctcCCTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTccc      <  2:930464/139‑1 (MQ=255)                                                                                                                               cTGCGTTAACGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGat  <  1:113025/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                   CATTATTACGGTTTTCATCACCGTCTGTTCCGCTGGCGACGTTCCGGCATGAGCGCCACCTGGTGTGCGCTGTTGCTGATGGTGCTGGCGAATCTTTGGTATATGCCCTGGGAGTTGGTTTTCTCCCTGCGTTAGCGCACAATACGCCACTTTCTTTTTCCCGGATTTTTACGTTATGTCTCACCGCGACACGCTATTTTCTGCCCCTATCGCCAGACTGGGCGACTGGACCTTTGATGAACGGGTAGCTGAAGTCTTCCCGGAT  >  NC_000913/1952527‑1952791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑