Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 183,620 G→A *386* (TAG→TAA cdaR → carbohydrate diacid regulon transcriptional regulator, autoregulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913183,6200GA100.0% 116.8 / NA 37*386* (TAG→TAAcdaRcarbohydrate diacid regulon transcriptional regulator, autoregulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/18);  total (19/18)

AAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183485‑183755
                                                                                                                                       |                                                                                                                                       
aaaGGCGTTCTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     <  2:633830/139‑1 (MQ=255)
       ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTa                                                                                                                              >  1:1075784/1‑139 (MQ=255)
       ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTa                                                                                                                              >  2:483359/1‑139 (MQ=255)
                  tCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggcg                                                                                                                   >  2:96931/1‑139 (MQ=255)
                          cccTGGCGTATCGGCTTAATCGTATATCGCAACTGACCGGGCTTGATGTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTTAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           <  2:1075784/139‑1 (MQ=255)
                          cccTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           <  1:483359/139‑1 (MQ=255)
                             tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     >  1:390409/1‑110 (MQ=255)
                             tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     <  2:390409/110‑1 (MQ=255)
                             tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                                     >  1:181642/1‑126 (MQ=255)
                             tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAgg                                                                                                                     <  2:181642/126‑1 (MQ=255)
                                             tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:195098/139‑1 (MQ=255)
                                                    gtataagagACAGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       >  1:12292/9‑117 (MQ=255)
                                                    gtataagagACAGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       <  2:12292/109‑1 (MQ=255)
                                                         aCTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAggc                                                                                                                    <  1:651282/99‑1 (MQ=255)
                                                         aCTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGGTATGCGTTAAGGTTGAggc                                                                                                                    >  2:651282/1‑99 (MQ=255)
                                                             aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  2:942289/1‑139 (MQ=255)
                                                              ccGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGcc                                                                       <  1:942289/139‑1 (MQ=255)
                                                                            ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     <  2:690670/63‑1 (MQ=255)
                                                                            ggCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTa                                                                                                                                     >  1:690670/1‑63 (MQ=255)
                                                                                             gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGtt                                                                                                                                      >  1:791088/1‑45 (MQ=255)
                                                                                             gTTGCTGCTGTATGTGGCGTTACAACTGGTGAAGAGCGGTAAGtt                                                                                                                                      <  2:791088/45‑1 (MQ=255)
                                                                                                       tATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           <  1:415153/62‑1 (MQ=255)
                                                                                                       tATGTGGCGTTACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           >  2:415153/1‑62 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                                             <  2:411149/66‑1 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGc                                                                                             >  1:411149/1‑66 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  2:235519/1‑139 (MQ=255)
                                                                                                                 tACAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  1:393561/1‑139 (MQ=255)
                                                                                                                   cAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           >  2:819203/1‑50 (MQ=255)
                                                                                                                   cAACTGGTGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGc                                                                                                           <  1:819203/50‑1 (MQ=255)
                                                                                                                             aaGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCg         <  1:96931/139‑1 (MQ=255)
                                                                                                                                agCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   <  2:374748/46‑1 (MQ=255)
                                                                                                                                agCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTccc                                                                                                   >  1:374748/1‑46 (MQ=255)
                                                                                                                                  cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:1061429/55‑1 (MQ=255)
                                                                                                                                  cGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        >  2:1061429/1‑55 (MQ=255)
                                                                                                                                    gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          <  2:222120/51‑1 (MQ=255)
                                                                                                                                    gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          >  1:222120/1‑51 (MQ=255)
                                                                                                                                    gTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCt  >  1:666562/1‑139 (MQ=255)
                                                                                                                                       |                                                                                                                                       
AAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACTGGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NC_000913/183485‑183755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: