Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,130,853 C→T S9N (AGC→AAC) 
*406* (TAG→TAA
wcaD ←
wcaC ←
putative colanic acid polymerase
putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,130,8530CT100.0% 80.2 / NA 26S9N (AGC→AAC) 
*406* (TAG→TAA
wcaD
wcaC
putative colanic acid polymerase
putative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

GATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGG  >  NC_000913/2130729‑2130972
                                                                                                                            |                                                                                                                       
gATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATa                                                                                                           <  1:63597/139‑1 (MQ=255)
           aaaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACAt                                                                                                <  2:1076386/139‑1 (MQ=255)
            aaaCAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATa                                                                                               <  1:601858/139‑1 (MQ=255)
                 gcagTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATActcct                                                                                          >  1:274427/1‑139 (MQ=255)
                        cAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                   <  2:1073326/139‑1 (MQ=255)
                        cAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCAt                                                                                   <  2:428157/139‑1 (MQ=255)
                          aGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCt                                                                                 >  2:583910/1‑139 (MQ=255)
                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                            >  1:42492/1‑139 (MQ=255)
                               ggTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCAGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                            >  1:705529/1‑139 (MQ=255)
                                gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGTATCtg                                                                                <  2:516942/134‑1 (MQ=255)
                                gTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGTATCtg                                                                                >  1:516942/1‑134 (MQ=255)
                                           aCGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAggcg                                                                <  2:42492/139‑1 (MQ=255)
                                                   gATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCt                                                        >  1:938358/1‑139 (MQ=255)
                                                         gAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTg                                                  >  1:833049/1‑139 (MQ=255)
                                                                tCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGc                                                                                     <  1:249403/97‑1 (MQ=255)
                                                                tCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGc                                                                                     >  2:249403/1‑97 (MQ=255)
                                                                  gCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTc                                         <  1:583910/139‑1 (MQ=255)
                                                                          gggCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCg                                 <  2:274427/139‑1 (MQ=255)
                                                                             cGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                         >  2:404828/1‑64 (MQ=255)
                                                                             cGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGa                                                                                                         <  1:404828/64‑1 (MQ=255)
                                                                                  ttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                            <  2:48458/88‑1 (MQ=255)
                                                                                  ttGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCtgttgt                                                                            >  1:48458/1‑88 (MQ=255)
                                                                                            ccAGCAGATAAATCAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCt               >  2:838129/1‑139 (MQ=255)
                                                                                                         cAGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCgg  <  2:993891/139‑1 (MQ=255)
                                                                                                          aGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTgcg            >  2:469686/1‑128 (MQ=255)
                                                                                                          aGCGGCAGCAACAGGTAGTTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTgcg            <  1:469686/128‑1 (MQ=255)
                                                                                                                            |                                                                                                                       
GATGCGTTCTAAAAACAGCAGTAACAAGACAGGTAAAAAAGTGACGATGGTGATGGGGAAACTTTCGCCAAGCTGGGCGATTTTGACGTTAACCAGCAGATAAATCAGCGGCAGCAACAGGTAGCTACAGATTCTGATAGAAGTTGACATACTCCTCCAGCATCTGTTGTCCACTGTAGGCGGCGCGGCTGCGTTGGCTGAACTCAGCCAGCGTGGTACCAAATATCGCCTGCGCGATTTCCGG  >  NC_000913/2130729‑2130972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: