Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,182,035 C→T L314L (CTA→TTA) 
*249* (TAG→TAA
yegV →
yegW ←
putative kinase
putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,182,0350CT100.0% 91.1 / NA 29L314L (CTA→TTA) 
*249* (TAG→TAA
yegV
yegW
putative kinase
putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/14);  total (15/14)

CGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGA  >  NC_000913/2181899‑2182161
                                                                                                                                        |                                                                                                                              
cgACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCGCTGGTATGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGCGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAatta                                                                                                                              >  1:719094/1‑139 (MQ=255)
       cATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCg                                                                                                                       <  1:379036/139‑1 (MQ=255)
             ggTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGcacaca                                                                                                                 <  1:178213/139‑1 (MQ=255)
               tGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACaaa                                                                                                               <  1:172576/139‑1 (MQ=255)
               tGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACaaa                                                                                                               >  2:605630/1‑139 (MQ=255)
                        tGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGtataa                                                                                                      <  2:552507/139‑2 (MQ=255)
                               cTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTg                                                                                               <  1:627862/139‑1 (MQ=255)
                                 ggCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCg                                                                                             >  2:631903/1‑139 (MQ=255)
                                             gCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAAt                                                                                 >  2:287100/1‑139 (MQ=255)
                                              ccACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATg                                                                                <  1:605630/139‑1 (MQ=255)
                                                            cGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAgg                                                                  <  2:124748/139‑1 (MQ=255)
                                                                     gggCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTg                                                         >  2:361556/1‑139 (MQ=255)
                                                                      ggCAATGCAGTGGCGTCGTGCGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGATTTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                          <  2:707581/105‑1 (MQ=255)
                                                                      ggCAATGCAGTGGCGTCGAGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACa                                                                                          >  1:707581/1‑105 (MQ=255)
                                                                           tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTActcct                                                                                                                         <  2:449637/69‑1 (MQ=255)
                                                                           tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTActcct                                                                                                                         >  1:449637/1‑69 (MQ=255)
                                                                           tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGcaca                                                                                                                   >  1:99693/1‑75 (MQ=255)
                                                                           tGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGcaca                                                                                                                   <  2:99693/75‑1 (MQ=255)
                                                                           tGCAGTGGCGTCGTGGGTTGACGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTc                                                   >  2:24515/1‑139 (MQ=255)
                                                                                   cgtcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCa                                           <  2:719094/139‑1 (MQ=255)
                                                                                     tcgtGGGTTGTCGTGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTa                                                                           <  1:641784/105‑1 (MQ=255)
                                                                                     tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTg                                                                                               <  2:465116/85‑1 (MQ=255)
                                                                                     tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTg                                                                                               >  1:465116/1‑85 (MQ=255)
                                                                                     tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTa                                                                           >  2:641784/1‑105 (MQ=255)
                                                                                     tcgtGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCAct                                         >  1:146182/1‑139 (MQ=255)
                                                                                        tGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCActtgc                                      >  1:104747/1‑139 (MQ=255)
                                                                                                              gTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                     <  1:685944/70‑1 (MQ=255)
                                                                                                              gTGATTGTGCGCCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGc                                                                                     >  2:685944/1‑70 (MQ=255)
                                                                                                                       cgcCAACGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTa       >  2:664701/1‑139 (MQ=255)
                                                                                                                            aCGCGCGAGGAATTACTCCTCGCACACAAAAACGTATAAATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGa  >  2:706363/1‑139 (MQ=255)
                                                                                                                                        |                                                                                                                              
CGACAGTCATGCCGGTGGCGTACTTGCCGGGCTGGCCTCTGGTCTGCCACTGGCGGATGCCGTATTACTGGGCAATGCAGTGGCGTCGTGGGTTGTCGGGCATCGGGGCGGTGATTGTGCGCCAACGCGCGAGGAACTACTCCTCGCACACAAAAACGTATAGATCGCTGCGACAGTGGCTAATGCTGTACTCAATAGGCCGTTGCTGTTGGTCAAGCGCCACTTGCTTGATCACCAGCACCGGTATTTTGCTATCTAACTGA  >  NC_000913/2181899‑2182161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: