Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,266,013	T→C	R188R (CGT→CGC)	yeiP →	elongation factor P‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,266,013	0	T	C	100.0%	120.9 / NA	36	R188R (CGT→CGC)	yeiP	elongation factor P‑like protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/18);  total (18/18)

GGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGC  >  NC_000913/2265887‑2266144                                                                                                                               |                                                                                                                                    ggCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACtt                                                                                                                         <  2:88421/139‑1 (MQ=255)      cTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGcc                                                                                                                    <  2:495244/139‑1 (MQ=255)          cAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCAt                                                                                                                <  2:289602/139‑1 (MQ=255)            gcgcCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGc                                                                                                              >  1:166049/1‑139 (MQ=255)                 cGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                         <  1:458524/139‑1 (MQ=255)                   tAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAg                                                                                                       >  2:819719/1‑139 (MQ=255)                   tAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAg                                                                                                       >  1:592091/1‑139 (MQ=255)                      cAAACCGGCGACATTGAGCACGGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGa                                                                                                    <  1:819719/139‑1 (MQ=255)                                           tggtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCttttt                                                                               >  1:912374/1‑139 (MQ=255)                                             gtctggtGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTa                                                                             <  2:912374/139‑1 (MQ=255)                                                       tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                     <  1:213946/105‑1 (MQ=255)                                                       tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                     >  2:213946/1‑105 (MQ=255)                                                       tCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATtata                                                                   >  2:163592/1‑139 (MQ=255)                                                        cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                  <  1:163592/139‑1 (MQ=255)                                                        cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                  <  1:826406/139‑1 (MQ=255)                                                        cAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATAc                                                                  <  1:188901/139‑1 (MQ=255)                                                          ggTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                         <  1:67576/98‑1 (MQ=255)                                                          ggTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGaaa                                                                                                         >  2:67576/1‑98 (MQ=255)                                                                         aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCa                                                                                                                       >  1:32113/1‑69 (MQ=255)                                                                         aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCa                                                                                                                       <  2:32113/69‑1 (MQ=255)                                                                         aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGcc                                                                                                                    >  2:237989/1‑72 (MQ=255)                                                                         aaGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGcc                                                                                                                    <  1:237989/72‑1 (MQ=255)                                                                          aGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTgcgc                                                <  2:609020/139‑1 (MQ=255)                                                                                ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                          >  1:8970/1‑139 (MQ=255)                                                                                ggCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTg                                          >  2:25999/1‑139 (MQ=255)                                                                                                tCCATATCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTc                          <  1:25999/139‑1 (MQ=255)                                                                                                     atCGAAGAACGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAttt                     >  1:522529/1‑139 (MQ=255)                                                                                                           gaaCGCCGTTCTAAGCGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTATTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTc               >  2:719290/1‑139 (MQ=255)                                                                                                           gaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                     >  1:565127/1‑53 (MQ=255)                                                                                                           gaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAggg                                                                                                     <  2:565127/53‑1 (MQ=255)                                                                                                           gaaCGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTc               >  2:588100/1‑139 (MQ=255)                                                                                                              cGCCGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTGAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGtt            >  2:463346/1‑139 (MQ=255)                                                                                                                 cGTTATATGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGttgat         >  1:822519/1‑139 (MQ=255)                                                                                                                     atatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTtgattgat     <  1:153726/139‑1 (MQ=255)                                                                                                                      tatGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATc    >  1:171261/1‑139 (MQ=255)                                                                                                                        tGGGGCGCGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCGTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGc  <  1:463346/139‑1 (MQ=255)                                                                                                                            gcgcgcTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAAtttt                    <  2:615137/117‑1 (MQ=255)                                                                                                                               |                                                                                                                                    GGCATCTGCCAGCGCCCGTAACAAACCGGCGACATTGAGCACTGGTCTGGTGATTCAGGTACCAGAATACTTAAGCCCGGGCGAAAAAATTCGTATCCATATCGAAGAACGCCGTTATATGGGGCGTGCTGACTAACTTCAGCCGCATGCAGAAAAGGGATAGCTCAGGCTGTCCCTTTTTTAATTTATTATACCAATCTTCTATTTTGCGCTTCCTGAACACCTTACGCCCTCAATTTTCACTCGTTGATTGATCGC  >  NC_000913/2265887‑2266144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑