Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,514,077	T→C	F346L (TTC→CTC)	nupC →	nucleoside (except guanosine) transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,514,077	0	T	C	100.0%	182.8 / NA	52	F346L (TTC→CTC)	nupC	nucleoside (except guanosine) transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (25/27);  total (25/27)

TTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTG  >  NC_000913/2513943‑2514211                                                                                                                                       |                                                                                                                                       ttccttccAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCt                                                                                                                                    >  1:687341/1‑139 (MQ=255) ttccttccAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTAGAGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGCAGGCATCATCTCTGTGCTCCt                                                                                                                                    >  2:626444/1‑139 (MQ=255)           tGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTc                                                                                                                          >  1:209963/1‑139 (MQ=255)            gAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCg                                                                                                                         <  1:243613/139‑1 (MQ=255)                      aaGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTtctc                                                                                                               <  2:398888/139‑1 (MQ=255)                          gggCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCa                                                                                                           <  2:209963/139‑1 (MQ=255)                            gCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         >  2:784428/1‑139 (MQ=255)                            gCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         <  1:626444/139‑1 (MQ=255)                                  tCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGAt                                                                                                   >  1:347457/1‑139 (MQ=255)                                     tGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTAt                                                                                                >  2:650829/1‑139 (MQ=255)                                        cGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTctct                                                                                                              >  1:458430/1‑122 (MQ=255)                                        cGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTctct                                                                                                              <  2:458430/122‑1 (MQ=255)                                          aCCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAg                                                                                           >  2:738412/1‑139 (MQ=255)                                                   gTTTGCAAGGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTa                                                                                  <  2:579169/139‑1 (MQ=255)                                                             aGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaa                                                                        >  2:590470/1‑139 (MQ=255)                                                               ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTaaa                                                                                <  2:183583/129‑1 (MQ=255)                                                               ttCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTaaa                                                                                >  1:183583/1‑129 (MQ=255)                                                                    tGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAagag                                                                 <  1:590470/139‑1 (MQ=255)                                                                         tgatgGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAgg                                                            <  1:513106/139‑1 (MQ=255)                                                                              gATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACg                                                       <  1:650829/139‑1 (MQ=255)                                                                                    cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCtt                                                                                                             <  1:690108/79‑1 (MQ=255)                                                                                    cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCtt                                                                                                             >  2:690108/1‑79 (MQ=255)                                                                                    cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGttt                                                 >  1:805850/1‑139 (MQ=255)                                                                                    cAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGttt                                                 >  1:947845/1‑139 (MQ=255)                                                                                     aGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTtc                                                <  2:947845/139‑1 (MQ=255)                                                                                         aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCt                                                                                                                            >  1:571267/1‑59 (MQ=255)                                                                                         aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCt                                                                                                                            <  2:571267/59‑1 (MQ=255)                                                                                         aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCa                                                               >  1:415641/1‑120 (MQ=255)                                                                                         aaTTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCa                                                               <  2:415641/120‑1 (MQ=255)                                                                                           ttGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCtt                                          <  1:738412/139‑1 (MQ=255)                                                                                                           tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGttt                                                                                                                               >  2:823722/1‑38 (MQ=255)                                                                                                           tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGttt                                                                                                                               <  1:823722/38‑1 (MQ=255)                                                                                                           tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGt                                                                                         <  1:90019/76‑1 (MQ=255)                                                                                                           tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGt                                                                                         >  2:90019/1‑76 (MQ=255)                                                                                                           tcCGCGTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGtt                          >  2:859126/1‑139 (MQ=255)                                                                                                             cgcgTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTa                        <  2:347457/139‑1 (MQ=255)                                                                                                             cgcgTGCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTa                        <  2:805850/139‑1 (MQ=255)                                                                                                                  gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  1:859126/139‑1 (MQ=255)                                                                                                                  gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  2:577503/139‑1 (MQ=255)                                                                                                                  gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  2:687341/139‑1 (MQ=255)                                                                                                                  gCTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  1:359388/139‑1 (MQ=255)                                                                                                                   cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         >  2:212446/1‑52 (MQ=255)                                                                                                                   cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAAt                                                                                                         <  1:212446/52‑1 (MQ=255)                                                                                                                   cTGAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGctc                  >  2:1054240/1‑139 (MQ=255)                                                                                                                     gAAGGCATCATCTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   >  1:805110/1‑136 (MQ=255)                                                                                                                     gAAGGCATCATCTCTCTGCTCCTGCTTTCCTTCGCTAACTTCTCTTCAATCGTGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGct                   <  2:805110/136‑1 (MQ=255)                                                                                                                           atcatcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTgg          >  2:41456/1‑139 (MQ=255)                                                                                                                             catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaatgaa                                                                    >  1:262203/1‑79 (MQ=255)                                                                                                                             catcTCTGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCtgaatgaa                                                                    <  2:262203/79‑1 (MQ=255)                                                                                                                                ctctGTGCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAgt     >  2:334369/1‑139 (MQ=255)                                                                                                                                   tgtgCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGtgtg  >  2:766224/1‑139 (MQ=255)                                                                                                                                   tgtgCTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGtgtg  <  1:20903/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAACGAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCTGGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGGTTTCTCGCTTCGGTCTGAAGCTGGTTTACGGCTCTACCCTGGTGAGTGTG  >  NC_000913/2513943‑2514211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑