| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,525,930 | C→T | *309* (TAG→TAA) | yfeR ← | transcriptional regulator of yefH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,525,930 | 0 | C | T | 100.0% | 167.1 / NA | 52 | *309* (TAG→TAA) | yfeR | transcriptional regulator of yefH |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (29/23); total (29/23) | |||||||||||
ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG > NC_000913/2525794‑2526059 | caacatcacacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTta > 2:377504‑M2/133‑139 (MQ=255) cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt > 1:331087‑M2/125‑139 (MQ=255) cacattactacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGAt > 1:861328‑M2/125‑139 (MQ=255) ctacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACgg > 2:701317‑M2/118‑139 (MQ=255) tacggggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGa > 1:63939‑M2/117‑139 (MQ=255) ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc < 2:953582‑M2/28‑1 (MQ=255) ggattcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATcccc > 1:953582‑M2/112‑139 (MQ=255) attcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCtt > 2:984383‑M2/110‑139 (MQ=255) ttcagtatcgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTc > 1:791077‑M2/109‑139 (MQ=255) cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc < 1:216419‑M2/11‑1 (MQ=255) cgatatgcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc > 2:216419‑M2/101‑111 (MQ=255) gcggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTa < 2:861328‑M2/45‑1 (MQ=255) cggatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAg > 1:1045088‑M2/94‑139 (MQ=255) gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt < 1:1040098‑M2/44‑1 (MQ=255) gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCAtt > 2:1040098‑M2/92‑135 (MQ=255) gatgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAgcg > 2:323206‑M2/92‑139 (MQ=255) tgacaaactgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCGGCCATTAGCGCa < 2:63939‑M2/50‑1 (MQ=255) actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 2:723007‑M2/56‑1 (MQ=255) actgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 2:1012010‑M2/84‑139 (MQ=255) tgggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg < 2:294823‑M2/58‑1 (MQ=255) gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc < 1:1046449‑M2/11‑1 (MQ=255) gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATc > 2:1046449‑M2/81‑91 (MQ=255) gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg > 2:330350‑M2/81‑139 (MQ=255) gggcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTgg < 1:330350‑M2/59‑1 (MQ=255) gcgaagatttttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGtc > 1:300799‑M2/79‑139 (MQ=255) tttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCAc < 1:984383‑M2/70‑1 (MQ=255) ttaccaggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg < 1:377504‑M2/71‑1 (MQ=255) ggatgcgataatctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg < 1:758483‑M2/58‑1 (MQ=255) ggatgcgataatctactccatcaaatttaacttctgattgattaatgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTg > 2:758483‑M2/63‑120 (MQ=255) atctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg < 2:791077‑M2/87‑1 (MQ=255) tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg < 1:492818‑M2/71‑1 (MQ=255) tctactccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACg > 2:492818‑M2/52‑122 (MQ=255) tccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 1:685629‑M2/47‑102 (MQ=255) tccatcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 2:685629‑M2/56‑1 (MQ=255) tcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat > 1:438619‑M2/43‑50 (MQ=255) tcaaatttaacttctgattgattactgccggggctcccggcaCACTTtat < 2:438619‑M2/8‑1 (MQ=255) tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc > 2:859192‑M2/43‑98 (MQ=255) tcaaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGccc < 1:859192‑M2/56‑1 (MQ=255) aaatttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGaaa < 1:1012010‑M2/99‑1 (MQ=255) tttaacttctgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTg < 2:121937‑M2/102‑1 (MQ=255) tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg < 1:269406‑M2/87‑1 (MQ=255) tgattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAg > 2:269406‑M2/29‑115 (MQ=255) ggattgattactgccggggctcacggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTAAGCGTCGGTGGAAAGTGACCGGttct > 2:213727‑M2/29‑139 (MQ=255) gattgattactgccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCGCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGGACGGttctt > 2:634190‑M2/28‑139 (MQ=255) gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa > 2:110311‑M2/17‑66 (MQ=255) gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 1:110311‑M2/50‑1 (MQ=255) gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGc < 2:701988‑M2/54‑1 (MQ=255) gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGc > 1:701988‑M2/17‑70 (MQ=255) gccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCa > 2:915919‑M2/17‑139 (MQ=255) ccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa > 1:980548‑M2/16‑65 (MQ=255) ccggggctcccggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCa < 2:980548‑M2/50‑1 (MQ=255) cggcaCACTTTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTgg > 1:808998‑M2/6‑139 (MQ=255) | ACATTACTACGGGGATTCAGTATCGATATGCGGATGACAAACTGGGCGAAGATTTTTACCAGGATGCGATAATCTACTCCATCAAATTTAACTTCTGATTGATTACTGCCGGGGCTCCCGGCAGTATGAATTCACTCTATATCTGATACAACGGATCCCCTTCCCGCCCAGCCATTAGCGCATTGCCCTGGTCTCGTACCACGTCCCATAAAGCTTCAGCGGCGGTGGAAAGTGAACGGTTCTTCCTGCGCACCAGCATCAACTGG > NC_000913/2525794‑2526059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |