Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,595,227 T→C Q211Q (CAA→CAG tmcA ← elongator methionine tRNA (ac4C34) acetyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,595,2270TC100.0% 54.2 / NA 17Q211Q (CAA→CAGtmcAelongator methionine tRNA (ac4C34) acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/10);  total (7/10)

TGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCAGTC  >  NC_000913/2595106‑2595358
                                                                                                                         |                                                                                                                                   
tGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGa                                                                                                                    <  2:512841/139‑1 (MQ=255)
     aaCAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTAc                                                                                                               <  2:651713/139‑1 (MQ=255)
            cATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGccc                                                                                                        >  2:293939/1‑139 (MQ=255)
                 ggCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCAcgcg                                                                                                   <  2:965236/139‑1 (MQ=255)
                  gcgcAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACcg                                                                                                             <  1:820670/128‑1 (MQ=255)
                  gcgcAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACcg                                                                                                             >  2:820670/1‑128 (MQ=255)
                                               aaTTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                                     <  2:815932/139‑1 (MQ=255)
                                                          tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc                                                          <  2:114249/139‑1 (MQ=255)
                                                          tACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgc                                                          <  1:293939/139‑1 (MQ=255)
                                                              tCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAgctgct                                                      >  1:324830/1‑139 (MQ=255)
                                                                    gACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAaga                                                >  1:74975/1‑139 (MQ=255)
                                                                         ttttGCGGGCGCGGTGACAATCGCTCTGCCCTCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACgcgc                                                                                                  >  1:538218/1‑84 (MQ=255)
                                                                         ttttGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACgcgc                                                                                                  <  2:538218/84‑1 (MQ=255)
                                                                         ttttGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGc                                           >  2:696025/1‑139 (MQ=255)
                                                                                                                  aaaTGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACcagtc  <  1:696025/139‑1 (MQ=255)
                                                                                                                    aTGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                                     <  1:81365/70‑1 (MQ=255)
                                                                                                                    aTGAGCTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCa                                                                     >  2:81365/1‑70 (MQ=255)
                                                                                                                         |                                                                                                                                   
TGGCTAACAAGGCATCCGGCGCAATAAAGCGAAACTTCTCGCCCGCAAATTGTGCCAGTACATCCGTTGACGCTTTTGCGGGCGCGGTGACAATCGCTCTGCCCGCAATACGAGAAATGAGTTGCCCTGCCAACGCCGACTTACCGCGCCCACGCGCAGCCGTTACCGCTGCCACGCCCGGCGGCATGGTCATTAGCTGCTTTAAGAGTTGCTGTTGTTCTGGTTGTGGTGCGCCAGTCGCGGGGTACCAGTC  >  NC_000913/2595106‑2595358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: