Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,618,075	C→T	*234* (TAG→TAA)	hda ←	ATPase regulatory factor involved in DnaA inactivation

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,618,075	0	C	T	100.0%	174.2 / NA	54	*234* (TAG→TAA)	hda	ATPase regulatory factor involved in DnaA inactivation
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/29);  total (25/29)

CATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCC  >  NC_000913/2617947‑2618209                                                                                                                                 |                                                                                                                                       cATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCa                                                                                                                              >  2:256783/1‑139 (MQ=255)  aTCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAg                                                                                                                             >  2:253118/1‑139 (MQ=255)   tCGCTAGTTGAATCGCATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGa                                                                                                                            <  2:416031/139‑1 (MQ=255)   tCGCTAGTTGAAGCACATTGCCAGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGa                                                                                                                            >  2:860337/1‑139 (MQ=255)      cTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTAGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAAtt                                                                                                                         >  2:636591/1‑139 (MQ=255)         gTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAAtttct                                                                                                                      >  1:366158/1‑139 (MQ=255)           tGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAAtttcttt                                                                                                                    <  1:860337/139‑1 (MQ=255)                   aTTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACg                                                                                                            >  1:690122/1‑139 (MQ=255)                     tGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGa                                                                                                          >  2:1034305/1‑139 (MQ=255)                                        cACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                       <  1:394123/139‑1 (MQ=255)                                                    ggCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                           <  1:619994/139‑1 (MQ=255)                                                     gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGc                                                                          <  1:636591/139‑1 (MQ=255)                                                      ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTa                                                                                                                                      <  1:933039/78‑1 (MQ=255)                                                      ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAAttt                                                                                                                        <  2:886310/92‑1 (MQ=255)                                                      ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAAttt                                                                                                                        >  1:886310/1‑92 (MQ=255)                                                      ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         <  1:455950/139‑1 (MQ=255)                                                      ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         <  1:253118/139‑1 (MQ=255)                                                      ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         <  1:1034305/139‑1 (MQ=255)                                                         aCGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGa                                                                      <  2:246078/139‑1 (MQ=255)                                                          cGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCACCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGAt                                                                     <  2:690122/139‑1 (MQ=255)                                                                   tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTa                                                                                                                                      >  2:263767/1‑65 (MQ=255)                                                                   tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTa                                                                                                                                      <  1:263767/65‑1 (MQ=255)                                                                    ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                           >  2:584174/1‑139 (MQ=255)                                                                     gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         <  2:121325/124‑1 (MQ=255)                                                                     gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         >  1:121325/1‑124 (MQ=255)                                                                      aTTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                         <  2:366158/139‑1 (MQ=255)                                                                       ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         <  1:847241/122‑1 (MQ=255)                                                                       ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         >  2:847241/1‑122 (MQ=255)                                                                         aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACg                                                      <  2:552179/139‑1 (MQ=255)                                                                          aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                      >  1:204784/1‑90 (MQ=255)                                                                          aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                      <  2:204784/90‑1 (MQ=255)                                                                          aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         <  1:436233/119‑1 (MQ=255)                                                                          aCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                         >  2:436233/1‑119 (MQ=255)                                                                             tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTg                                                                                         >  2:1003119/1‑100 (MQ=255)                                                                             tCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTg                                                                                         <  1:1003119/100‑1 (MQ=255)                                                                                cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGt                                                                                                     <  1:246885/85‑1 (MQ=255)                                                                                cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGt                                                                                                     >  2:246885/1‑85 (MQ=255)                                                                                     ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                 <  1:408066/116‑1 (MQ=255)                                                                                     ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCaa                                                                 >  2:408066/1‑116 (MQ=255)                                                                                         gATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                      >  2:320372/1‑139 (MQ=255)                                                                                         gATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                      <  1:320372/139‑1 (MQ=255)                                                                                               gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                       >  2:302943/1‑84 (MQ=255)                                                                                               gCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                       <  1:302943/84‑1 (MQ=255)                                                                                                    cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACaa                                                                                                               >  2:528077/1‑55 (MQ=255)                                                                                                    cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACaa                                                                                                               <  1:528077/55‑1 (MQ=255)                                                                                                    cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                         >  2:169771/1‑109 (MQ=255)                                                                                                    cgcgCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                         <  1:169771/109‑1 (MQ=255)                                                                                                         cgcATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCa                                    <  2:214635/125‑1 (MQ=255)                                                                                                         cgcATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCa                                    >  1:214635/1‑125 (MQ=255)                                                                                                                 aCAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAg              >  2:14498/1‑139 (MQ=255)                                                                                                                        acacCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATg                                                                                                       <  1:978779/43‑1 (MQ=255)                                                                                                                        acacCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATg                                                                                                       >  2:978779/1‑43 (MQ=255)                                                                                                                           ccTTATTTACAACTTCAGAATGTCTTTCACAAACGGAATTGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGTGCCGCTTCAGCAAGAAACGc    <  2:631310/139‑1 (MQ=255)                                                                                                                             ttATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTGTGTCGAGCCGCTTCAGCAAGAAACGccc  >  2:174919/1‑139 (MQ=255)                                                                                                                                 |                                                                                                                                       CATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCC  >  NC_000913/2617947‑2618209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑