Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,685,910 G→A A26T (GCC→ACC)  hmp → fused nitric oxide dioxygenase/dihydropteridine reductase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,685,9100GA100.0% 187.6 / NA 59A26T (GCC→ACC) hmpfused nitric oxide dioxygenase/dihydropteridine reductase 2
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/25);  total (34/25)

GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACG  >  NC_000913/2685774‑2686027
                                                                                                                                        |                                                                                                                     
gCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAaccacc                                                                                                                     <  2:982371/139‑1 (MQ=255)
 cTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                    <  1:99836/139‑1 (MQ=255)
 cTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                    >  1:924676/1‑139 (MQ=255)
            aaGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                         <  2:124976/139‑1 (MQ=255)
               aTGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGt                                                                                                      <  1:1067888/139‑1 (MQ=255)
                tGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                    <  1:59457/124‑1 (MQ=255)
                tGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                    >  2:59457/1‑124 (MQ=255)
                         gaTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTc                                                                                            >  2:1034392/1‑139 (MQ=255)
                          aTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAATGTTAACCACCCATTTCTACGACCg                                                                                                       >  2:943146/1‑127 (MQ=255)
                          aTACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAATGTTAACCACCCATTTCTACGACCg                                                                                                       <  1:943146/127‑1 (MQ=255)
                              aTCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                       <  1:1046176/123‑1 (MQ=255)
                              aTCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                       >  2:1046176/1‑123 (MQ=255)
                               tCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAAcc                                                                                      >  1:547936/1‑139 (MQ=255)
                                   ttAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                    >  1:454565/1‑105 (MQ=255)
                                   ttAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCAccc                                                                                                                    <  2:454565/105‑1 (MQ=255)
                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             >  1:667171/1‑107 (MQ=255)
                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             <  2:667171/107‑1 (MQ=255)
                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                         <  2:848456/111‑1 (MQ=255)
                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGAc                                                                                                         >  1:848456/1‑111 (MQ=255)
                                        aTGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCa                                                                             >  1:536414/1‑139 (MQ=255)
                                             aaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             >  1:89763/1‑102 (MQ=255)
                                             aaaaaaGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             <  2:89763/102‑1 (MQ=255)
                                                       gACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCt                                                                                                              >  2:746842/1‑91 (MQ=255)
                                                       gACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCt                                                                                                              <  1:746842/91‑1 (MQ=255)
                                                        aCCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                       >  1:872313/1‑97 (MQ=255)
                                                        aCCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCg                                                                                                       <  2:872313/97‑1 (MQ=255)
                                                                  tGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAg                                                   >  2:193823/1‑139 (MQ=255)
                                                                   gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             >  2:525654/1‑80 (MQ=255)
                                                                   gACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             <  1:525654/80‑1 (MQ=255)
                                                                                     aCAGTAAAAGCCACCATCCCTGTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTATCATGAGTAACCAGCGTAATGGCGATCAACGTg                                <  2:569042/139‑1 (MQ=255)
                                                                                      cAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGa                               >  1:111562/1‑139 (MQ=255)
                                                                                         tAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAAc                                   <  2:674460/132‑1 (MQ=255)
                                                                                         tAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAAc                                   >  1:674460/1‑132 (MQ=255)
                                                                                          aaaaGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGcc                           <  2:924676/139‑1 (MQ=255)
                                                                                              gCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTaa                                              <  1:746386/116‑1 (MQ=255)
                                                                                              gCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTaa                                              >  2:746386/1‑116 (MQ=255)
                                                                                                 accaTCCCTTTCATGGTGGAAACGGGGACAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTa                    >  2:72707/1‑139 (MQ=255)
                                                                                                 accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             >  2:806910/1‑50 (MQ=255)
                                                                                                 accaTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTa                                                                                                             <  1:806910/50‑1 (MQ=255)
                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAaccacc                                                                                                                     <  1:823128/40‑1 (MQ=255)
                                                                                                   caTCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAAc                  >  1:976311/1‑139 (MQ=255)
                                                                                                             cTGGTGGGAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccg        >  1:885929/1‑139 (MQ=255)
                                                                                                             cTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                           <  1:1075266/56‑1 (MQ=255)
                                                                                                             cTGGTGGAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCa                                                                                           >  2:1075266/1‑56 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                <  2:197779/48‑1 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGttt                                                                                                >  1:197779/1‑48 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccgcc      <  2:806966/138‑1 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTgccgcc      >  1:806966/1‑138 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt     >  2:673156/1‑139 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt     >  2:804654/1‑139 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCt     >  1:68043/1‑139 (MQ=255)
                                                                                                                gtggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGATATGGCCGCCt     >  2:863794/1‑139 (MQ=255)
                                                                                                                  ggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                 >  2:448136/1‑45 (MQ=255)
                                                                                                                  ggAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGtt                                                                                                 <  1:448136/45‑1 (MQ=255)
                                                                                                                   gAAACGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACg  >  2:316670/1‑139 (MQ=255)
                                                                                                                     aaCGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATaa                                                                                        <  2:980189/51‑1 (MQ=255)
                                                                                                                     aaCGGGGCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATaa                                                                                        >  1:980189/1‑51 (MQ=255)
                                                                                                                          ggCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAAcc                                                                                      <  1:419954/48‑1 (MQ=255)
                                                                                                                          ggCCAAAGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAAcc                                                                                      >  2:419954/1‑48 (MQ=255)
                                                                                                                               aaGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGc                            <  1:290778/101‑1 (MQ=255)
                                                                                                                               aaGTTAACCACCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGc                            >  2:290778/1‑101 (MQ=255)
                                                                                                                                        |                                                                                                                     
GCTATTTTTTATAAGATGCATTTGAGATACATCAATTAAGATGCAAAAAAAGGAAGACCATATGCTTGACGCTCAAACCATCGCTACAGTAAAAGCCACCATCCCTTTACTGGTGGAAACGGGGCCAAAGTTAACCGCCCATTTCTACGACCGTATGTTTACTCATAACCCAGAACTCAAAGAAATTTTTAACATGAGTAACCAGCGTAATGGCGATCAACGTGAAGCCCTGTTTAACGCTATTGCCGCCTACG  >  NC_000913/2685774‑2686027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑