Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	255,659	A→G	C20R (TGT→CGT)	pepD ←	aminoacyl‑histidine dipeptidase (peptidase D)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	255,659	0	A	G	100.0%	214.7 / NA	60	C20R (TGT→CGT)	pepD	aminoacyl‑histidine dipeptidase (peptidase D)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/23);  total (37/23)

CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793                                                                                                                                  |                                                                                                                                       cGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGc                                                                                                                               >  1:541088/1‑139 (MQ=255)     aGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCaaaa                                                                                                                           <  1:755797/139‑1 (MQ=255)        aGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAata                                                                                                                        >  1:77959/1‑139 (MQ=255)         ggTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAatat                                                                                                                       <  1:390118/139‑1 (MQ=255)         ggTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAatat                                                                                                                       <  1:977950/139‑1 (MQ=255)                              cctacctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATa                                                                                                  >  1:1038343/1‑139 (MQ=255)                                 acctGATCGCGTTCGACATGGAAACATTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAAtt                                                                                               >  2:980942/1‑139 (MQ=255)                                   ctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTga                                                                                             >  1:214112/1‑139 (MQ=255)                                   ctGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTga                                                                                             >  2:193701/1‑139 (MQ=255)                                     gATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  1:95782/1‑139 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  1:1030564/138‑1 (MQ=255)                                      aTCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  2:1030564/1‑138 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     <  1:82269/126‑1 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGgag                                                                                                     >  2:82269/1‑126 (MQ=255)                                        cgcgTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAg                                                                                        >  1:625169/1‑139 (MQ=255)                                                             tctcTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTgg                                                                                                                                >  1:289402/1‑78 (MQ=255)                                                             tctcTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTgg                                                                                                                                <  2:289402/78‑1 (MQ=255)                                                                      cccAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCa                                                          >  1:469420/1‑139 (MQ=255)                                                                                 aTGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             <  2:896789/77‑1 (MQ=255)                                                                                 aTGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCgg                                                                                                             >  1:896789/1‑77 (MQ=255)                                                                                    tATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            >  2:1037142/1‑139 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                <  1:463569/70‑1 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAg                                                                                                                >  2:463569/1‑70 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            >  2:99899/1‑138 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTa                                            <  1:99899/138‑1 (MQ=255)                                                                                     aTTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTAt                                           >  1:43749/1‑139 (MQ=255)                                                                                      ttCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTAtt                                          >  1:565269/1‑139 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       <  1:193701/139‑1 (MQ=255)                                                                                         aGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCa                                       <  2:1038343/139‑1 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           <  2:835434/86‑1 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTgaga                                                                                           >  1:835434/1‑86 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAg                                      >  1:944939/1‑139 (MQ=255)                                                                                          gCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAg                                      >  2:159612/1‑139 (MQ=255)                                                                                            gAGTTGCCCTTCATGATAGGACGGGCGAGGAATAGAACGGATTTTGGCAAAAATATCCCACCGCGGCTGTGGAGATCATTGAGACAGTTCAGAGCCGTTAAGTCTCCTTGTCGATCACCCGCAAATAAGTATAGCAGGt                                    >  2:436126/1‑139 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGc                                                                                                            >  2:457584/1‑65 (MQ=255)                                                                                              gTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGc                                                                                                            <  1:457584/65‑1 (MQ=255)                                                                                               ttGCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTcac                                 <  2:214112/139‑1 (MQ=255)                                                                                                 gCTCTTCATGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAg                               >  1:1023853/1‑139 (MQ=255)                                                                                                        aTGATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGc                        <  2:95782/139‑1 (MQ=255)                                                                                                          gATAGGACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTGGTCGATCACCCGCAAAACAGTATTGCAGGGCACAGGGTtagtag                      >  2:215817/1‑139 (MQ=255)                                                                                                               gACGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAt                 <  1:159612/139‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTcc                                                                    >  1:979829/1‑86 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTcc                                                                    <  2:979829/86‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   >  2:796564/1‑103 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCaa                                                   <  1:796564/103‑1 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgt               >  1:281249/1‑139 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgt               >  2:747577/1‑139 (MQ=255)                                                                                                                 cGGGTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgt               >  1:136731/1‑139 (MQ=255)                                                                                                                   ggTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttg             >  1:442086/1‑139 (MQ=255)                                                                                                                   ggTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttg             >  1:131833/1‑139 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGt                                                                        <  2:30058/79‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGt                                                                        >  1:30058/1‑79 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGt                            >  2:387343/1‑123 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGt                            <  1:387343/123‑1 (MQ=255)                                                                                                                    gTGAGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAAtgttgt            >  1:421686/1‑139 (MQ=255)                                                                                                                       aGGAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCaa         <  2:1023853/139‑1 (MQ=255)                                                                                                                         gAATAGAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAaca       >  1:511548/1‑139 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          >  2:876440/1‑51 (MQ=255)                                                                                                                              gAACGGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGAc                                                                                          <  1:876440/51‑1 (MQ=255)                                                                                                                              gAACGGATGTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGa  <  1:436126/139‑1 (MQ=255)                                                                                                                                  |                                                                                                                                       CGGTAGCAGGTTTACGAATCAGGATATTACCTACCTGATCGCGTTCGACATGGAAACCTTTCTCTTTTGCCCAACCAACAATGTATTCAGCGAGTTGCTCTTCATGATAGGACGGGTGAGGAATAGAACAGATTTTGGCAAAAATATCCCACAGCGGCTGTGGAGATAATTGAGACAGTTCAGACACGTTAAGTCTCCTTGTCGATCACCCGCAAAACAGTATTGCAGGTCACAGGGTTAGCAGAAAATGTTGTCAACACAAGA  >  NC_000913/255530‑255793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑