Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,796,916 T→C Y38C (TAT→TGT)  yqaE ← cyaR sRNA‑regulated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,796,9160TC100.0% 154.0 / NA 43Y38C (TAT→TGT) yqaEcyaR sRNA‑regulated protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/25);  total (18/25)

AGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATATACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAAT  >  NC_000913/2796787‑2797049
                                                                                                                                 |                                                                                                                                     
aGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCa                                                                                                                              <  2:786558/139‑1 (MQ=255)
    aCCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAgtgt                                                                                                                          >  2:273542/1‑139 (MQ=255)
     ccTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTc                                                                                                                         >  2:645848/1‑139 (MQ=255)
       tCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCaa                                                                                                                       <  1:614172/139‑1 (MQ=255)
          ttCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAg                                                                                                                    >  2:497246/1‑139 (MQ=255)
          ttCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAg                                                                                                                    <  1:473707/139‑1 (MQ=255)
          ttCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAg                                                                                                                    >  2:154569/1‑139 (MQ=255)
            cGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGaa                                                                                                                  >  2:495183/1‑139 (MQ=255)
            cGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGaa                                                                                                                  <  1:497246/139‑1 (MQ=255)
                       acacTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATg                                                                                                       <  1:154569/139‑1 (MQ=255)
                           tCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACg                                                                                                   >  1:298512/1‑139 (MQ=255)
                             aGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGcc                                                                                                 <  2:30299/139‑1 (MQ=255)
                                     tAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCaa                                                                                                                                 >  1:26308/1‑99 (MQ=255)
                                     tAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCaa                                                                                                                                 <  2:26308/99‑1 (MQ=255)
                                     tAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGaa                                                                                         <  1:495183/139‑1 (MQ=255)
                                     tAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGaa                                                                                         >  2:349302/1‑139 (MQ=255)
                                           agGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGa                                                                                          <  2:18199/132‑1 (MQ=255)
                                           agGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGa                                                                                          >  1:18199/1‑132 (MQ=255)
                                             gAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGa                                                                                                                   <  1:766246/105‑1 (MQ=255)
                                             gAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGa                                                                                                                   >  2:766246/1‑105 (MQ=255)
                                                 gcggcgCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGag                                                                             >  2:721406/1‑139 (MQ=255)
                                                   ggcgCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGc                                                                                                                               >  2:580919/1‑87 (MQ=255)
                                                   ggcgCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGc                                                                                                                               <  1:580919/87‑1 (MQ=255)
                                                                cgcgGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAgcgg                                                              <  1:645848/139‑1 (MQ=255)
                                                                   ggggATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGcggcgg                                                           <  1:349302/139‑1 (MQ=255)
                                                                          aGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAAcc                                                                                            <  1:876307/99‑1 (MQ=255)
                                                                          aGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAAcc                                                                                            >  2:876307/1‑99 (MQ=255)
                                                                             acaGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAatgatg                                                 <  1:273542/139‑1 (MQ=255)
                                                                                      aTCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCaa                                                                                                                       <  2:213004/60‑1 (MQ=255)
                                                                                      aTCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCaa                                                                                                                       >  1:213004/1‑60 (MQ=255)
                                                                                      aTCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACg                                        >  1:958123/1‑139 (MQ=255)
                                                                                         gcgGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAACAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGAtt                                     <  2:322924/139‑1 (MQ=255)
                                                                                                  cACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGaaa                            <  2:298512/139‑1 (MQ=255)
                                                                                                  cACCCAGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGaaa                            <  1:862901/139‑1 (MQ=255)
                                                                                                       aGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAgcgg                                                              >  2:305812/1‑100 (MQ=255)
                                                                                                       aGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAgcgg                                                              <  1:305812/100‑1 (MQ=255)
                                                                                                       aGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGa                              <  1:851380/132‑1 (MQ=255)
                                                                                                       aGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGa                              >  2:851380/1‑132 (MQ=255)
                                                                                                       aGAACGCGTGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCat                       <  1:721406/139‑1 (MQ=255)
                                                                                                               tGAATCAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTc               >  2:55042/1‑139 (MQ=255)
                                                                                                                    cAAACCGGGGATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCtat          <  1:55042/139‑1 (MQ=255)
                                                                                                                          ggggATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGaaa    <  2:958123/139‑1 (MQ=255)
                                                                                                                            ggATACACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAAt  <  1:85715/139‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
AGCTACCTCATTCGTTAACGGAAACACTCAGAAAGTGTAGCAGAGGAACGCGGCGCGTTAACGCCGCGGGGATGAGCACAGGTTTAATCGCGGGTTTGCACCCAGAACGCGTGAATCAAACCGGGGATATACCCAAGCAGTGTCAACAGAATATTAATAATGAACGCCCAACCGAACCCTTTACCGAGCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAAT  >  NC_000913/2796787‑2797049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: