Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,800,475 G→A *110* (TAG→TAA ygaM → putative membrane‑anchored DUF883 family ribosome‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,800,4750GA100.0% 78.3 / NA 26*110* (TAG→TAAygaMputative membrane‑anchored DUF883 family ribosome‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/14);  total (12/14)

CGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC  >  NC_000913/2800367‑2800606
                                                                                                            |                                                                                                                                   
cGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGACAATAAATACGCGTCTTTg                                                                                                       <  2:597900/139‑1 (MQ=255)
       tGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGTTGCGGTAGGTCTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGTCCCCGa                                                                                                <  2:684801/139‑1 (MQ=255)
                        tCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGtt                                                                               >  1:560793/1‑139 (MQ=255)
                                               tGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaa                                                        >  1:136537/1‑139 (MQ=255)
                                               tGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaa                                                        >  1:310238/1‑139 (MQ=255)
                                                 ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGaa                                                                                               >  1:1031851/1‑98 (MQ=255)
                                                 ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGaa                                                                                               <  2:1031851/98‑1 (MQ=255)
                                                 ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                      >  1:303857/1‑139 (MQ=255)
                                                 ggTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACaaaa                                                      >  2:389452/1‑139 (MQ=255)
                                                     cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATaataaat                                                                                                                  <  1:558963/75‑1 (MQ=255)
                                                     cagcagCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATaataaat                                                                                                                  >  2:558963/1‑75 (MQ=255)
                                                      agcagcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATccc                                                 >  2:564045/1‑139 (MQ=255)
                                                          gcTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTa                                             >  1:292244/1‑139 (MQ=255)
                                                           cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGa                                                                                                                          <  1:539255/61‑1 (MQ=255)
                                                           cTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGa                                                                                                                          >  2:539255/1‑61 (MQ=255)
                                                                gtaggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCTTACCCCGt                                       <  2:250783/139‑1 (MQ=255)
                                                                  aggtaTTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCa                                     <  1:564045/139‑1 (MQ=255)
                                                                                               tGCGCAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACt        <  1:399099/139‑1 (MQ=255)
                                                                                                gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                             <  1:370414/53‑1 (MQ=255)
                                                                                                gcgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGc                                                                                             >  2:370414/1‑53 (MQ=255)
                                                                                                 cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  2:310238/139‑1 (MQ=255)
                                                                                                 cgcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAt      <  2:303857/139‑1 (MQ=255)
                                                                                                  gcAAATCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTAtt     >  2:674391/1‑139 (MQ=255)
                                                                                                    aaaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTg   <  2:292244/139‑1 (MQ=255)
                                                                                                     aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGATCCCGAAGCCTGTCTTCGGGGTTTCTTCTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTGCGACCACTATTTGc  <  1:652358/139‑1 (MQ=255)
                                                                                                     aaTCGTAATGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGc  <  1:981209/139‑1 (MQ=255)
                                                                                                            |                                                                                                                                   
CGTTGGCTGCGCGGATTCTTTTGTTCGTGAAAGACCCTGGTGTAGCGTGGGTACAGCAGCTGCGGTAGGTATTTTTATCGGCGCACTGTTAAGCATGCGCAAATCGTAGTGCAAAAATGATAATAAATACGCGTCTTTGACCCCGAAGCCTGTCTTCGGGGTTTCTTTTTGCCTGGTGAATCACAAAAATCCCCCTACCCCGTCACGCTCATATCCAGGGTAATTTCGACCACTATTTGC  >  NC_000913/2800367‑2800606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: