| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,811,495 | G→A | L23L (CTG→CTA) | emrA → | multidrug efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,811,495 | 0 | G | A | 89.8% | 120.2 / 6.5 | 49 | L23L (CTG→CTA) | emrA | multidrug efflux system |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (22/22); total (24/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.87e-01 | |||||||||||
GCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAGGGAATCAAATTCAAAT > NC_000913/2811365‑2811617 | gCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTActcct < 2:338110/136‑1 (MQ=255)gCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTActcct > 1:338110/1‑136 (MQ=255) tGGCCTTTTTTGCAAGCAAGTCGGCTCAACCGATGAGTTAAGAAAATCCTTGAGAACCATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTtc < 1:248761/139‑1 (MQ=255) ggCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTtct < 1:449373/139‑1 (MQ=255) gCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTtc < 1:848360/137‑1 (MQ=255) gCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTtc > 2:848360/1‑137 (MQ=255) ttGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTTCTCACCTTg < 2:910460/139‑1 (MQ=255) ccGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTa > 1:14785/1‑139 (MQ=255) gTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAg < 2:14785/139‑1 (MQ=255) aTCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACGTTGCTCTTTATAATTATTGCCGTa > 1:267177/1‑123 (MQ=255) aTCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTCCTCACCTTGCTCTTTATAATTATTGCCGTa < 2:267177/123‑1 (MQ=255) tGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGtt > 1:247188/1‑139 (MQ=255) tGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTg > 2:446917/1‑86 (MQ=255) tGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTg < 1:446917/86‑1 (MQ=255) gAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTg > 2:279708/1‑85 (MQ=255) gAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTg < 1:279708/85‑1 (MQ=255) gcgcAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCa > 1:212407/1‑139 (MQ=255) gcAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCAcc > 1:215688/1‑78 (MQ=255) gcAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCAcc < 2:215688/78‑1 (MQ=255) cAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTg < 1:1071304/80‑1 (MQ=255) cAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTg > 2:1071304/1‑80 (MQ=255) cAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTgg > 1:333345/1‑126 (MQ=255) cAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTgg < 2:333345/126‑1 (MQ=255) aaTGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGct < 2:128397/80‑1 (MQ=255) aaTGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGct > 1:128397/1‑80 (MQ=255) tGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCgaa < 1:61140/139‑1 (MQ=255) cGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCgaaga > 2:426353/1‑139 (MQ=255) gagaCTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGa < 2:791885/97‑1 (MQ=255) gagaCTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGa > 1:791885/1‑97 (MQ=255) aCTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCg < 2:247188/139‑1 (MQ=255) aaaCCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGa > 1:321440/1‑139 (MQ=255) cccGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCa < 2:212407/139‑1 (MQ=255) cGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTg > 2:938562/1‑97 (MQ=255) cGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTg < 1:938562/97‑1 (MQ=255) cGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATa > 2:975536/1‑139 (MQ=255) cagcaACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCgaag < 1:834810/121‑1 (MQ=255) cagcaACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCgaag > 2:834810/1‑121 (MQ=255) cagcaACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACg < 1:975536/139‑1 (MQ=255) aagaGCGGCAAACGTAAGCGTCTACT‑CTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGAc > 2:417666/1‑116 (MQ=255) aagaGCGGCAAACGTAAGCGTCTACT‑CTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGAc > 2:573635/1‑116 (MQ=255) aagaGCGGCAAACGTAAGCGTCTACT‑CTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGAc < 1:573635/116‑1 (MQ=255) aagaGCGGCAAACGTAAGCGTCTACT‑CTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGAc < 1:417666/116‑1 (MQ=255) agagCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAgg < 1:670944/130‑1 (MQ=255) agagCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAgg > 2:670944/1‑130 (MQ=255) gagCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAGGGAAtcaaatt > 2:876073/1‑139 (MQ=255) gCGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAGGGAAtcaaattca > 1:104790/1‑139 (MQ=255) cGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTtat > 2:548517/1‑45 (MQ=255) cGGCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTtat < 1:548517/45‑1 (MQ=255) gCAAACGTAAGCGTCTACTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAGGGAAtcaaattcaaat < 1:261451/139‑1 (MQ=255) | GCTGGCCTTTTTGGCAAGCAGGTCGGCTCAGCCGATGAGTTAAGAAGATCGTGGAGAACAATATGAGCGCAAATGCGGAGACTCAAACCCCGCAGCAACCGGTAAAGAAGAGCGGCAAACGTAAGCGTCTGCTCCTCCTTCTCACCTTGCTCTTTATAATTATTGCCGTAGCGATAGGGATTTATTGGTTTTTGGTACTGCGTCACTTCGAAGAAACCGATGACGCATACGTGGCAGGGAATCAAATTCAAAT > NC_000913/2811365‑2811617 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |