Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,859,538	C→T	L816L (CTG→TTG)	mutS →	methyl‑directed mismatch repair protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,859,538	0	C	T	100.0%	126.8 / NA	40	L816L (CTG→TTG)	mutS	methyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/22);  total (18/22)

CTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTC  >  NC_000913/2859409‑2859672                                                                                                                                  |                                                                                                                                       cTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTAc                                                                                                                               <  1:267771/139‑1 (MQ=255)          gCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGaaa                                                                                                                      >  1:879651/1‑139 (MQ=255)                                   agGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGa                                                                                             <  2:879651/139‑1 (MQ=255)                                     gTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCt                                                                                                              >  1:986954/1‑120 (MQ=255)                                     gTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                                                           >  1:774089/1‑139 (MQ=255)                                     gTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGAAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCt                                                                                                              <  2:986954/120‑1 (MQ=255)                                            aGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGa                                                                                    <  1:96009/139‑1 (MQ=255)                                              cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                  <  1:109815/139‑1 (MQ=255)                                              cgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                  <  1:1015117/139‑1 (MQ=255)                                               gcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCg                                                                                                       >  2:790981/1‑117 (MQ=255)                                               gcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCg                                                                                                       <  1:790981/117‑1 (MQ=255)                                               gcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATcc                                                                                 >  1:525329/1‑139 (MQ=255)                                                  cACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                                                              >  1:47236/1‑139 (MQ=255)                                                       cAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                  >  1:590022/1‑130 (MQ=255)                                                       cAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATc                                                                                  <  2:590022/130‑1 (MQ=255)                                                               gCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcg                                                                 >  1:428382/1‑139 (MQ=255)                                                                 gTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAg                                                                                                    <  1:461533/102‑1 (MQ=255)                                                                 gTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAg                                                                                                    >  2:461533/1‑102 (MQ=255)                                                                 gTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcgcg                                                               >  1:391167/1‑139 (MQ=255)                                                                      cTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGa                                                                                             <  1:116498/104‑1 (MQ=255)                                                                      cTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGa                                                                                             >  2:116498/1‑104 (MQ=255)                                                                        ggAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcg                                                        >  1:958227/1‑139 (MQ=255)                                                                                   cGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGc                                                                                                                <  2:95816/72‑1 (MQ=255)                                                                                   cGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGc                                                                                                                >  1:95816/1‑72 (MQ=255)                                                                                     gcGAACGCCGCCGCTACGCAAGGTGATGGTACTCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTACGGGTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAttt                                           <  2:774089/138‑1 (MQ=255)                                                                                     ccGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAttt                                           <  2:460582/139‑1 (MQ=255)                                                                                              gccgcTACGCAAGT‑GATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                                 <  1:518958/139‑1 (MQ=255)                                                                                               ccgcTACGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGaa                                 <  1:746867/139‑1 (MQ=255)                                                                                                    aCGCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGcc                            <  2:428382/139‑1 (MQ=255)                                                                                                      gCAAGTGGATGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCC‑GATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTgg                         <  1:904134/139‑1 (MQ=255)                                                                                                               tGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGcc                            >  2:13871/1‑128 (MQ=255)                                                                                                               tGGTACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGcc                            <  1:13871/128‑1 (MQ=255)                                                                                                                  tACGCAAATGTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACaa              <  2:391167/139‑1 (MQ=255)                                                                                                                           gTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAg                                                  >  2:920372/1‑94 (MQ=255)                                                                                                                           gTCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAg                                                  <  1:920372/94‑1 (MQ=255)                                                                                                                            tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcact                                                                        <  2:170991/71‑1 (MQ=255)                                                                                                                            tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcact                                                                        >  1:170991/1‑71 (MQ=255)                                                                                                                            tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCACTTGAAGAGCCTGGTGTAATAACAATTCCCGa       >  2:926182/1‑136 (MQ=255)                                                                                                                            tCTTTGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCACTTGAAGAGCCTGGTGTAATAACAATTCCCGa       <  1:926182/136‑1 (MQ=255)                                                                                                                              tttGTTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTc  >  2:689120/1‑139 (MQ=255)                                                                                                                                  |                                                                                                                                       CTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTTGAAGAGCCTGGTGTAATAACAATTCCCGATAGTC  >  NC_000913/2859409‑2859672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑