Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,929,455	C→T	N409N (AAC→AAT)	sdaC →	putative serine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,929,455	0	C	T	100.0%	168.8 / NA	53	N409N (AAC→AAT)	sdaC	putative serine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/25);  total (28/25)

AGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGCGTATTC  >  NC_000913/2929333‑2929590                                                                                                                           |                                                                                                                                        aGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTAtt                                                                                                                                    >  2:402545/1‑128 (MQ=255) aGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTAtt                                                                                                                                    <  1:402545/128‑1 (MQ=255)       cTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTc                                                                                                                   <  1:217488/139‑1 (MQ=255)                aTTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                          >  2:460211/1‑123 (MQ=255)                aTTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTg                                                                                                                          <  1:460211/123‑1 (MQ=255)                aTTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCaa                                                                                                          >  1:776508/1‑139 (MQ=255)                 ttGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAAt                                                                                                         >  1:871910/1‑139 (MQ=255)                    aaaCCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAAtctc                                                                                                      >  1:959561/1‑139 (MQ=255)                        ccTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  <  2:871910/139‑1 (MQ=255)                          tGGGCGGTCCAATCATCGCGATGATTCTGTTGCGGATGCCGATGTACGCAATTCAGAAAGCATCGGCAATGCGTAAGTACAGCGGGCACATCAGCAATGTATTTGTTGTCGTGAGGGGTCTGTTTGCAATCTCAGCAAt                                                                                                <  2:104392/139‑1 (MQ=255)                                 tCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCACGTCAGAAAGTACCGGCCATGCGCAAGTACAGCAGTCACATCGGCAGTGTATTCGTTGTCGTGATGGGTCCGATTGCAATCTCCGCAATCTTCTAc                                                                                         >  2:867811/1‑139 (MQ=255)                                  ccAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTAct                                                                                        <  1:667624/139‑1 (MQ=255)                                           gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCaa                                                                                                          <  2:293411/112‑1 (MQ=255)                                           gcgATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCaa                                                                                                          >  1:293411/1‑112 (MQ=255)                                               tgatCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTa                                                                           >  2:183659/1‑139 (MQ=255)                                                           tGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTcgcg                                                               >  1:261663/1‑139 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  >  2:73893/1‑103 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  <  1:73893/103‑1 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGt                                                                                  <  2:1009913/119‑1 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGt                                                                                  >  1:1009913/1‑119 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCgcgc                                                              <  2:776508/139‑1 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCgcgc                                                              >  2:1065598/1‑139 (MQ=255)                                                            gATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCgcgc                                                              >  2:328414/1‑139 (MQ=255)                                                             aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                                >  2:544100/1‑104 (MQ=255)                                                             aTGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAt                                                                                                <  1:544100/104‑1 (MQ=255)                                                                       aCGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGggc                                                   <  2:713282/139‑1 (MQ=255)                                                                           aaTTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATg                                                                                                                       >  2:106561/1‑67 (MQ=255)                                                                           aaTTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATg                                                                                                                       <  1:106561/67‑1 (MQ=255)                                                                                aGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcc                                          >  2:470788/1‑139 (MQ=255)                                                                                  aaaGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcctc                                        >  1:869677/1‑139 (MQ=255)                                                                                       aCCGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGttt                                   >  2:179602/1‑139 (MQ=255)                                                                                        ccGGCAATGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGtttt                                  <  1:328414/139‑1 (MQ=255)                                                                                             aaTGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcgc                             <  1:168229/139‑1 (MQ=255)                                                                                               tGCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGa                           <  2:869677/139‑1 (MQ=255)                                                                                                gCGTAAGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGAt                          <  1:1065598/139‑1 (MQ=255)                                                                                                    aaGTACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt                      <  1:183659/139‑1 (MQ=255)                                                                                                       tACAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTc                   >  2:698849/1‑139 (MQ=255)                                                                                                         cAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt                      >  2:277936/1‑134 (MQ=255)                                                                                                         cAGCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTAt                      <  1:277936/134‑1 (MQ=255)                                                                                                           gCGGTCACATCAGCATTGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTAt               >  2:752306/1‑139 (MQ=255)                                                                                                           gCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAtctt                                                                                             >  2:251861/1‑61 (MQ=255)                                                                                                           gCGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAAtctt                                                                                             <  1:251861/61‑1 (MQ=255)                                                                                                            cGGTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATg              >  2:673079/1‑139 (MQ=255)                                                                                                              gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                                    <  1:11271/51‑1 (MQ=255)                                                                                                              gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCg                                                                                                    >  2:11271/1‑51 (MQ=255)                                                                                                              gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  <  2:303143/53‑1 (MQ=255)                                                                                                              gTCACATCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCa                                                                                                  >  1:303143/1‑53 (MQ=255)                                                                                                                 acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcct                                         <  1:676087/107‑1 (MQ=255)                                                                                                                 acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTtcct                                         >  2:676087/1‑107 (MQ=255)                                                                                                                 acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcg                              <  1:416058/118‑1 (MQ=255)                                                                                                                 acaTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAAcg                              >  2:416058/1‑118 (MQ=255)                                                                                                                  caTCAGCAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGc        <  2:959561/139‑1 (MQ=255)                                                                                                                        cAATGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGCGTATTc  >  2:964732/1‑139 (MQ=255)                                                                                                                           |                                                                                                                                        AGCATCCTGGGTATGATTGAAACCCTGGGCGGTCCAATCATCGCGATGATCCTGTTCCTGATGCCGATGTACGCAATTCAGAAAGTACCGGCAATGCGTAAGTACAGCGGTCACATCAGCAACGTATTCGTTGTCGTGATGGGTCTGATTGCAATCTCCGCAATCTTCTACTCTCTGTTCAGCTAAGTCCTTTCGCGCCGCTTTCGGGCGGCGCTTCCTCCGTTTTAACGCGATGTATTTCCTATGATTAGCGTATTC  >  NC_000913/2929333‑2929590

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑