Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,983,288	C→T	*279* (TAG→TAA)	kduI ←	hexuronate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,983,288	0	C	T	100.0%	202.6 / NA	63	*279* (TAG→TAA)	kduI	hexuronate isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/32);  total (31/32)

TGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCT  >  NC_000913/2983164‑2983421                                                                                                                             |                                                                                                                                      tGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCttt                                                                                                                         <  2:384961/139‑1 (MQ=255)        gCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCa                                                                                                                                <  1:127579/125‑1 (MQ=255)        gCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCa                                                                                                                                >  2:127579/1‑125 (MQ=255)         ccccAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCca                                                                                                                 >  1:259307/1‑139 (MQ=255)                  cATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGCGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                               <  2:770889/132‑1 (MQ=255)                  cATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                               >  1:770889/1‑132 (MQ=255)                         tGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATatcatc                                                                                                 >  1:484614/1‑139 (MQ=255)                               agtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                           <  2:259307/139‑1 (MQ=255)                               agtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAc                                                                                           >  1:537804/1‑139 (MQ=255)                                gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCca                                                                                                                 <  1:1010926/116‑1 (MQ=255)                                gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCca                                                                                                                 >  2:1010926/1‑116 (MQ=255)                                gtccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGAcc                                                                                          >  2:525257/1‑139 (MQ=255)                                 tccagtATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCt                                                                                         >  1:736662/1‑139 (MQ=255)                                           caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                >  1:934229/1‑106 (MQ=255)                                           caACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcac                                                                                                                <  2:934229/106‑1 (MQ=255)                                                    acgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                      <  2:537804/139‑1 (MQ=255)                                                     cgacCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGccc                                                                     <  1:291513/139‑1 (MQ=255)                                                                   ccctCGAGAGAGAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCTCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                     >  2:623420/4‑93 (MQ=255)                                                                   ccTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCatat                                                                                                     <  1:623420/93‑1 (MQ=255)                                                                        gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACg                                                                                                                     >  1:617953/1‑72 (MQ=255)                                                                        gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACg                                                                                                                     <  2:617953/72‑1 (MQ=255)                                                                        gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATAtcatca                                                                                                >  2:972180/1‑93 (MQ=255)                                                                        gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATAtcatca                                                                                                <  1:972180/93‑1 (MQ=255)                                                                        gagagaAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCtt                                                  >  1:814067/1‑139 (MQ=255)                                                                              aaaTGCACTTAAAATCATAACACTACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTGCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTc                                            <  1:224464/139‑1 (MQ=255)                                                                                   cACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGaca                                       <  1:368204/139‑1 (MQ=255)                                                                                       tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCt                                                   >  1:1018076/1‑123 (MQ=255)                                                                                       tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCt                                                   <  2:1018076/123‑1 (MQ=255)                                                                                       tAAAATCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCgg                                   >  1:705914/1‑139 (MQ=255)                                                                                        aaaaTCATAACAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGa                                  <  1:525257/139‑1 (MQ=255)                                                                                                aaCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCg                          >  1:244651/1‑139 (MQ=255)                                                                                                 aCAATACCTTATTCGTTTATGCCCACAGTTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                         <  2:705914/139‑1 (MQ=255)                                                                                                 aCAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                         <  2:814067/139‑1 (MQ=255)                                                                                                  cAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                        <  2:940341/139‑1 (MQ=255)                                                                                                  cAATACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGAc                        >  1:940341/1‑139 (MQ=255)                                                                                                    aTACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCa                      <  2:736662/139‑1 (MQ=255)                                                                                                     tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATgg                                                                                                            <  1:448720/52‑1 (MQ=255)                                                                                                     tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATgg                                                                                                            >  2:448720/1‑52 (MQ=255)                                                                                                     tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                      <  2:309211/90‑1 (MQ=255)                                                                                                     tACCTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGcc                                                                      >  1:309211/1‑90 (MQ=255)                                                                                                       ccTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                        >  1:846496/1‑54 (MQ=255)                                                                                                       ccTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCa                                                                                                        <  2:846496/54‑1 (MQ=255)                                                                                                        cTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                                    >  2:647801/1‑41 (MQ=255)                                                                                                        cTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACgg                                                                                                                    <  1:647801/41‑1 (MQ=255)                                                                                                        cTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                               <  1:553292/46‑1 (MQ=255)                                                                                                        cTTATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCcaca                                                                                                               >  2:553292/1‑46 (MQ=255)                                                                                                         ttATTCGTTTATGCCCACAATTAGCGCAAATCTTTaa                                                                                                                       >  1:583579/1‑37 (MQ=255)                                                                                                         ttATTCGTTTATGCCCACAATTAGCGCAAATCTTTaa                                                                                                                       <  2:583579/37‑1 (MQ=255)                                                                                                          tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                           >  1:1015303/1‑80 (MQ=255)                                                                                                          tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGAcc                                                                           <  2:1015303/80‑1 (MQ=255)                                                                                                          tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                               <  2:429666/92‑1 (MQ=255)                                                                                                          tATTCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATa                                                               >  1:429666/1‑92 (MQ=255)                                                                                                            ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                          <  2:778329/79‑1 (MQ=255)                                                                                                            ttCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCa                                                                          >  1:778329/1‑79 (MQ=255)                                                                                                             tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                         >  2:348744/1‑127 (MQ=255)                                                                                                             tCGTTTATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGa                         <  1:348744/127‑1 (MQ=255)                                                                                                                 ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATg                                                                                                             >  2:162378/1‑39 (MQ=38)                                                                                                                 ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATg                                                                                                             <  1:162378/39‑1 (MQ=255)                                                                                                                 ttATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATc         >  2:49415/1‑139 (MQ=255)                                                                                                                  tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGt                                                          <  2:196594/89‑1 (MQ=255)                                                                                                                  tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGt                                                          >  1:196594/1‑89 (MQ=255)                                                                                                                  tATGCCCACAATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCa        >  2:979030/1‑139 (MQ=255)                                                                                                                        cacaATTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCt  <  1:979030/139‑1 (MQ=255)                                                                                                                             |                                                                                                                                      TGCGCCAGCCCCAACGCCATCCCCTGACCCAGTCCAGTATCACAACCAGTGACGACCGCAACTTTACCTTCGAGAGAAAATGCACTTAAAATCATAACAATACCTTATTCGTTTATGCCCACAACTAGCGCAAATCTTTAACGGCCACATGGTCCATATCATCAAAGACCTGGTTTTCACCGACCATGCCCCAGATAAAGGTATAAGCTTTGGTTCCGACACCGGAATGGATCGACCAGCTCGGGGAGATCACCGCCT  >  NC_000913/2983164‑2983421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑