Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,056,273 G→A P11P (CCG→CCA)  fau → 5‑formyltetrahydrofolate cyclo‑ligase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,056,2730GA100.0% 160.3 / NA 50P11P (CCG→CCA) fau5‑formyltetrahydrofolate cyclo‑ligase family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (27/23);  total (27/23)

TACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACT  >  NC_000913/3056137‑3056395
                                                                                                                                        |                                                                                                                          
tACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGATAACGCCAGa                                                                                                                          >  1:474765/1‑139 (MQ=255)
     ccTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaac                                                                                                                     >  1:656843/1‑139 (MQ=255)
                               ttcttATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCt                                                                                           <  2:865059/139‑1 (MQ=255)
                                     tCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCgg                                                                                     >  2:396396/1‑139 (MQ=255)
                                     tCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCgg                                                                                     <  2:665250/139‑1 (MQ=255)
                                       tGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgat                                                                                   >  2:490225/1‑139 (MQ=255)
                                        ggCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGATCAACAAGCCGCTACCCGgatg                                                                                  >  1:377629/1‑139 (MQ=255)
                                          cACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGgatgat                                                                                <  2:929208/139‑1 (MQ=255)
                                               gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                      >  2:830318/1‑96 (MQ=255)
                                               gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                      <  1:830318/96‑1 (MQ=255)
                                               gCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACtt                                                                           >  2:292476/1‑139 (MQ=255)
                                                   tGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAGATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATcc                                                                       >  1:725067/1‑139 (MQ=255)
                                                   tGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATcc                                                                       >  1:320101/1‑139 (MQ=255)
                                                        cAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                        <  1:350345/85‑1 (MQ=255)
                                                        cAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaac                                                                                                                        >  2:350345/1‑85 (MQ=255)
                                                          aCTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCggtg                                                                >  1:325835/1‑139 (MQ=255)
                                                                aGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATg                                                          <  1:490225/139‑1 (MQ=255)
                                                                 gAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATgg                                                         >  1:277698/1‑139 (MQ=255)
                                                                     tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaa                                                                                                             <  2:114154/83‑1 (MQ=255)
                                                                     tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGaa                                                                                                             >  1:114154/1‑83 (MQ=255)
                                                                     tCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGcaca                                                     >  2:7005/1‑139 (MQ=255)
                                                                       ccACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATa                                                   >  2:356280/1‑139 (MQ=255)
                                                                        cACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                      <  1:1023728/71‑1 (MQ=255)
                                                                        cACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                      >  2:1023728/1‑71 (MQ=255)
                                                                         aCTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACg                                                 >  1:495458/1‑139 (MQ=255)
                                                                          cTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACgg                                                <  2:474765/139‑1 (MQ=255)
                                                                             aCATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCg                                             <  2:495458/139‑1 (MQ=255)
                                                                               aTTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCt                                           >  2:376463/1‑139 (MQ=255)
                                                                                 tATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGt                                         >  2:750813/1‑139 (MQ=255)
                                                                                  aTCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTa                                        <  1:356280/139‑1 (MQ=255)
                                                                                     ccGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTc                                     <  2:277698/139‑1 (MQ=255)
                                                                                       gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCct                                   >  1:850945/1‑139 (MQ=255)
                                                                                       gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCct                                   <  1:7005/139‑1 (MQ=255)
                                                                                       gACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCct                                   >  1:576445/1‑139 (MQ=255)
                                                                                        aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTcccc                                  >  1:810482/1‑137 (MQ=255)
                                                                                        aCAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCctc                                  >  1:440445/1‑139 (MQ=255)
                                                                                         cAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCctct                                 >  2:86537/1‑139 (MQ=255)
                                                                                             aaaTCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                      <  2:591697/50‑1 (MQ=255)
                                                                                             aaaTCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCAGaacaa                                                                                                                      >  1:591697/1‑50 (MQ=255)
                                                                                                         tGATTCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACt                 <  1:80419/139‑1 (MQ=255)
                                                                                                            ttCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATg                                                          >  1:491772/1‑95 (MQ=255)
                                                                                                            ttCGGCAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATg                                                          <  2:491772/95‑1 (MQ=255)
                                                                                                                 cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATgg                                                                                                         >  2:422306/1‑43 (MQ=255)
                                                                                                                 cAACGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATgg                                                                                                         <  1:422306/43‑1 (MQ=255)
                                                                                                                   aCGTCGTCGTGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAg       <  2:713263/139‑1 (MQ=255)
                                                                                                                     gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGcc     <  1:292476/139‑1 (MQ=255)
                                                                                                                     gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGcc     <  1:86537/139‑1 (MQ=255)
                                                                                                                     gtcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGcc     <  1:750813/139‑1 (MQ=255)
                                                                                                                     atcgtcgcACGTTAACGCCAGGACAGCGGCAGGAAATTGTTCAACACGCCGCTAGCCTGACGATGACTTAACCCACGGTGGTGATGGCACATAGCGTCGGTGTATTCCTGTCTTTTGATGGCGAACTCGACACCCacgc     <  2:767781/138‑4 (MQ=255)
                                                                                                                      tcgtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCa    <  2:850945/139‑1 (MQ=255)
                                                                                                                        gtcgtGCGTTAACGCCAGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACt  <  1:396396/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                          
TACAGCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGACATTATCCCGACAAGAAATCCGCAAAATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGACTTATCCCCCGGTGGTGATGGCACATACGGTCGCTGTATTCCTCTCTTTTGATGGCGAACTCGACACCCAGCCACT  >  NC_000913/3056137‑3056395

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑