Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,096,673	G→A	*97* (TAG→TAA)	yggU →	UPF0235 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,096,673	0	G	A	100.0%	48.3 / NA	17	*97* (TAG→TAA)	yggU	UPF0235 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/10);  total (7/10)

AAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATT  >  NC_000913/3096569‑3096792                                                                                                         |                                                                                                                        aaaaGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGc                                                                                       <  1:376290/139‑1 (MQ=255)   aaGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCaa                                                                                     <  1:438068/139‑1 (MQ=255)         ggtggtGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCgg                                                                               >  2:992373/1‑139 (MQ=255)          gtggtgATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTc                                                                                 <  1:768496/136‑1 (MQ=255)          gtggtgATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAAGCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTc                                                                                 >  2:768496/1‑136 (MQ=255)                                                           ttAATCCGTAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAGGTTGTCCTCGCAACCgg                                                                                        <  2:615626/80‑1 (MQ=255)                                                           ttAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCgg                                                                                        >  1:615626/1‑80 (MQ=255)                                                                   caacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATcc                                                                                                                   <  1:434488/45‑1 (MQ=255)                                                                   caacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATcc                                                                                                                   >  2:434488/1‑45 (MQ=255)                                                                   caacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCg                             >  1:70855/1‑131 (MQ=255)                                                                   caacaaATCCCGCCAGAAATCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCg                             <  2:70855/131‑1 (MQ=255)                                                                                      tCGCGGCGTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGAtt  <  1:992373/139‑1 (MQ=255)                                                                                             gTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTa                                                                             >  2:237976/1‑57 (MQ=255)                                                                                             gTTAATTAATTAAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTa                                                                             <  1:237976/57‑1 (MQ=255)                                                                                                      ttaAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCg                                                                      >  1:445465/1‑55 (MQ=255)                                                                                                      ttaAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCg                                                                      <  2:445465/55‑1 (MQ=255)                                                                                                      ttaAGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGt                            <  1:427539/97‑1 (MQ=255)                                                                                                         |                                                                                                                        AAAAGCCAGGTGGTGATTGAAAAAGGCGAACTTGGCCGCCACAAACAAATTAAAATCATTAATCCGCAACAAATCCCGCCAGAAATCGCGGCGTTAATTAATTAGGTATCCTATGCAAAAAGTTGTCCTCGCAACCGGCAATGTCGGTAAAGTGCGTGAGCTGGCGTCGCTGCTTAGCGACTTCGGTCTTGATATCGTGGCCCAAACAGACCTCGGCGTTGATT  >  NC_000913/3096569‑3096792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑