| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,122,095 | G→A | P571S (CCG→TCG) | glcB ← | malate synthase G |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,122,095 | 0 | G | A | 92.3% | 102.0 / 0.5 | 39 | P571S (CCG→TCG) | glcB | malate synthase G |
| Reads supporting (aligned to +/- strand): ref base G (1/2); new base A (18/18); total (19/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.99e-01 | |||||||||||
CTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCG > NC_000913/3121967‑3122222 | cTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCNTTTTCANNANCNGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCa > 1:921133/1‑139 (MQ=255) gCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTc > 2:427959/1‑130 (MQ=255) gCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTc < 1:427959/130‑1 (MQ=255) tCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCAt < 1:972053/139‑1 (MQ=255) cAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACt < 2:114431/139‑1 (MQ=255) gcACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGCTCAAATTCAGCATTGAACTCGGt < 1:496096/139‑1 (MQ=255) cacGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTgg > 1:314207/1‑139 (MQ=255) acGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTggg < 1:556786/139‑1 (MQ=255) ccccAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATg < 2:314207/139‑1 (MQ=255) ccAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGtt < 2:290389/139‑1 (MQ=255) ccTGCATGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTAc > 1:1067743/1‑139 (MQ=255) ccTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTAc > 2:713039/1‑139 (MQ=255) ctctTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGttggtttgg > 1:202999/1‑139 (MQ=255) gttgGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGttggttt < 2:857626/132‑1 (MQ=255) gttgGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGttggttt > 1:857626/1‑132 (MQ=255) aTCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCa < 1:540442/139‑1 (MQ=255) tGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACg < 2:529146/114‑1 (MQ=255) tGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACg > 1:529146/1‑114 (MQ=255) ccGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAg < 2:333965/139‑1 (MQ=255) aCCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGt > 1:719918/1‑139 (MQ=255) aCCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACg > 2:690039/1‑98 (MQ=255) aCCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACg < 1:690039/98‑1 (MQ=255) cAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAAc > 2:271438/1‑69 (MQ=255) cAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAAc < 1:271438/69‑1 (MQ=255) ttttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACg < 2:637071/87‑1 (MQ=255) ttttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACg > 1:637071/1‑87 (MQ=255) ttttCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTg < 1:124707/139‑1 (MQ=255) aTAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGCTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAgg < 2:817852/139‑1 (MQ=255) tAGTCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGc < 2:921133/139‑1 (MQ=255) tCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGc > 2:210640/1‑69 (MQ=255) tCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGc < 1:210640/69‑1 (MQ=255) tCAGCAGATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCtgt > 2:676215/1‑139 (MQ=255) agcagATCGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTgtgt > 2:332944/1‑139 (MQ=255) cGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGt < 2:108732/37‑1 (MQ=25) cGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGt > 1:108732/1‑37 (MQ=255) cGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTa > 2:1057590/1‑57 (MQ=255) cGTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTa < 1:1057590/57‑1 (MQ=255) gTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCg > 1:595218/1‑139 (MQ=255) gTCCAGCAGCGATTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCg > 1:519529/1‑139 (MQ=255) | CTGCTCCACCCAGCGCACCACGTACCCCAGAATCCCCTGCACGTTGTTATCCAGCTCTTGTTGGATCTCTTGCGCCGACCAGTTAGCGTTTTCAGCAACCGGAATAGTCAGCAGATCGTCCAGCAGCGGTTCAAATTCAGCATTGAACTCGGTCTGGGCAATGTTGGCTTGTACGCTCTGTACGTTGGTTTGGTGGTAGTGCAGCGCATGGAGCGTAGCAGCGGTTGGTGACGGAACCCAGGCTGTGTTTGCCCCG > NC_000913/3121967‑3122222 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |