Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,140,294	T→C	S9G (AGC→GGC)	hybF ←	protein involved with the maturation of hydrogenases 1 and 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,140,294	0	T	C	100.0%	60.3 / NA	18	S9G (AGC→GGC)	hybF	protein involved with the maturation of hydrogenases 1 and 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/7);  total (11/7)

CCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGG  >  NC_000913/3140158‑3140425                                                                                                                                         |                                                                                                                                    ccGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGccct                                                                                                                                   <  2:285062/139‑1 (MQ=255)    tGGCAGACAATTTCAACACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGc                                                                                                                                >  1:950132/1‑139 (MQ=255)                                            cAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCaa                                                                                        <  1:709911/139‑1 (MQ=255)                                                  aGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCATTCCTGCGACCAAAcagca                                                                                  <  1:706648/139‑1 (MQ=255)                                                       agCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGgcg                                                                             >  2:1065702/1‑139 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCaa                                                                                        <  1:580923/126‑1 (MQ=255)                                                         cgcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCaa                                                                                        >  2:580923/1‑126 (MQ=255)                                                          gcgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGa                                                                          <  1:468150/139‑1 (MQ=255)                                                           cgcCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGAc                                                                         >  2:543554/1‑139 (MQ=255)                                                                           cacGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTAc                                                         >  1:750203/1‑139 (MQ=255)                                                                                  gTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGa                                                  >  1:327020/1‑139 (MQ=255)                                                                                         gcTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGa                                           <  2:327020/139‑1 (MQ=255)                                                                                             aaCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCAt                                                                                                                 >  2:795165/1‑65 (MQ=255)                                                                                             aaCATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCAt                                                                                                                 <  1:795165/65‑1 (MQ=255)                                                                                                    tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGt                                                           <  2:798105/112‑1 (MQ=255)                                                                                                    tgctgcTCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGt                                                           >  1:798105/1‑112 (MQ=255)                                                                                                         ctCCGCCTGCCGTTGGATAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGAt                           >  2:108289/1‑139 (MQ=255)                                                                                                                         aTAATTTCAACGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCa           >  1:469372/1‑139 (MQ=255)                                                                                                                                  aCGGCGCCCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAgg  >  1:1060768/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                    CCGTGGCAGACAATTTCAAAACTAAAACGGACGGCGCTCTCCTCAACGCAGGAGAGCGCGCCAATTTCCAGCCACACGGCGGTGACGCGCTTAACATCGTGCTGCTCCGCCTGCCGTTGGATAATTTCAACGGCGCTCTGGCAAAGAGACAACTCATGCATTTTCGCCACTCCTGCGACCAAACAGCAGGGCGCGACGCCCTGCGTGTGGTACATCCGGATTCGTGACTGGCAGCGAAAGGATCATTCGTGCGCAGTCATCCGTCAGG  >  NC_000913/3140158‑3140425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑