Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,298,035	T→C	E35G (GAA→GGA)	yraQ ←	putative inner membrane permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,298,035	0	T	C	100.0%	204.7 / NA	57	E35G (GAA→GGA)	yraQ	putative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/30);  total (27/30)

TCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTT  >  NC_000913/3297928‑3298159                                                                                                            |                                                                                                                             tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCGCATTTCACATACCAGAGACCGGCAATGAc                                                                                               >  1:436014/1‑139 (MQ=255) tcGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGAc                                                                                               >  1:839407/1‑139 (MQ=255)  cgcgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAgaga                                                                                                          <  2:954221/127‑1 (MQ=255)  cgcgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAgaga                                                                                                          >  1:954221/1‑127 (MQ=255)    cgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                        >  1:781258/1‑111 (MQ=255)    cgTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCAtt                                                                                                                        <  2:781258/111‑1 (MQ=255)            aaCGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGTGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGagaaaga                                                                                    >  2:468788/1‑139 (MQ=255)                   ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCGCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             <  2:436014/139‑1 (MQ=255)                   ccTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             >  2:569350/1‑139 (MQ=255)                                 gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTcac                                                                                                                    <  1:589309/86‑1 (MQ=255)                                 gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTcac                                                                                                                    >  2:589309/1‑86 (MQ=255)                                 gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTc                                                                      <  1:482765/132‑1 (MQ=255)                                 gCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTc                                                                      >  2:482765/1‑132 (MQ=255)                                      cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTcac                                                                                                                    <  2:413926/81‑1 (MQ=255)                                      cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTcac                                                                                                                    >  1:413926/1‑81 (MQ=255)                                      cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             <  2:773402/120‑1 (MQ=255)                                      cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAgcgc                                                                             >  1:773402/1‑120 (MQ=255)                                      cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATg                                                          >  2:1019154/1‑139 (MQ=255)                                      cGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATg                                                          >  2:181412/1‑139 (MQ=255)                                       gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                             >  2:259273/1‑103 (MQ=255)                                       gCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACaa                                                                                             <  1:259273/103‑1 (MQ=255)                                                   aTAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             <  1:569350/139‑1 (MQ=255)                                                        tttACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGAt                                        <  1:181412/139‑1 (MQ=255)                                                           aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgag                                          <  1:64782/134‑1 (MQ=255)                                                           aCCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTgag                                          >  2:64782/1‑134 (MQ=255)                                                              gATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          >  1:677738/1‑51 (MQ=255)                                                              gATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          <  2:677738/51‑1 (MQ=255)                                                                 aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          >  1:710409/1‑48 (MQ=255)                                                                 aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCa                                                                                                                          <  2:710409/48‑1 (MQ=255)                                                                 aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               <  2:982355/139‑1 (MQ=255)                                                                 aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               <  1:468788/139‑1 (MQ=255)                                                                 aCTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               <  2:870045/139‑1 (MQ=255)                                                                  ctatgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                           >  1:791423/4‑94 (MQ=255)                                                                  ctatgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCgg                                                                           <  2:791423/91‑1 (MQ=255)                                                                  cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  1:87091/139‑1 (MQ=255)                                                                  cTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:839407/139‑1 (MQ=255)                                                                     tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACg                                                                                           >  2:969415/1‑75 (MQ=255)                                                                     tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACg                                                                                           <  1:969415/75‑1 (MQ=255)                                                                     tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             <  2:24064/121‑1 (MQ=255)                                                                     tgGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             >  1:24064/1‑121 (MQ=255)                                                                          ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               >  1:140062/1‑130 (MQ=255)                                                                          ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGa                               <  2:140062/130‑1 (MQ=255)                                                                          ttCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATa                      >  2:577032/1‑139 (MQ=255)                                                                           tCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAgaga                                                                                                          >  2:1865/1‑54 (MQ=255)                                                                           tCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAgaga                                                                                                          <  1:1865/54‑1 (MQ=255)                                                                             ggCAGCAGTGAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCa                   <  1:761313/139‑1 (MQ=255)                                                                                  cagTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTccc              <  1:1019154/139‑1 (MQ=255)                                                                                   agTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:975493/122‑1 (MQ=255)                                                                                   agTAAACGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              >  1:975493/1‑122 (MQ=255)                                                                                       aaCGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             <  2:982639/103‑1 (MQ=255)                                                                                       aaCGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCt                                             >  1:982639/1‑103 (MQ=255)                                                                                        aCGCTTTGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGaa        <  2:1019866/139‑1 (MQ=255)                                                                                              tGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              >  2:385497/1‑111 (MQ=255)                                                                                              tGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  1:385497/111‑1 (MQ=255)                                                                                              tGCCGTAGTAAGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGAATTCCCCAGGAATAAGtt  >  2:727995/1‑139 (MQ=255)                                                                                                        aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              <  2:516305/101‑1 (MQ=255)                                                                                                        aGGTCCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGAc                              >  1:516305/1‑101 (MQ=255)                                                                                                            |                                                                                                                             TCGCGTAATCCAACGCCGCCTGCCATGGGTTAGCATCCGCCTGCGCAAGGATAGATTTACCGATACTGTGGGTTTCGGCAGCAGTAAACGCTTTGCCGTAGTAAGGTTCCCATTTCACATACCAGAGACCGGCAATGACAACGAGAAAGAAAAGCGCGGGTTTCCACCACTGAATGGGCGTTGCCGCCTGAGATGAAGACTGACCAGTCATAGCATTCCCCAGGAATAAGTT  >  NC_000913/3297928‑3298159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑