Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,320,333	T→C	E427E (GAA→GAG)	yhbX ←	putative EptAB family phosphoethanolamine transferase, inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,320,333	0	T	C	100.0%	236.9 / NA	67	E427E (GAA→GAG)	yhbX	putative EptAB family phosphoethanolamine transferase, inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/36);  total (31/36)

AGATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAA  >  NC_000913/3320197‑3320466                                                                                                                                         |                                                                                                                                      aGATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTc                                                                                                                                     <  2:138824/139‑1 (MQ=255)                 tgtgCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCTATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     <  1:678869/139‑1 (MQ=255)                    gCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCaaaa                                                                                                                  <  1:368009/139‑1 (MQ=255)                     cGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCaa                                                                                                                    >  1:915353/1‑136 (MQ=255)                     cGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCaa                                                                                                                    <  2:915353/136‑1 (MQ=255)                               cATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGa                                                                                                       >  1:253891/1‑139 (MQ=255)                                  ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                                    >  1:796483/1‑139 (MQ=255)                                  ccccAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgc                                                                                                    >  2:918714/1‑139 (MQ=255)                                      aaGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcgg                                                                                                <  2:830094/139‑1 (MQ=255)                                       aGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               <  2:371298/139‑1 (MQ=255)                                        gAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggcg                                                                                              <  1:624383/139‑1 (MQ=255)                                             gggCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCtt                                                                                         >  1:13129/1‑139 (MQ=255)                                                        gATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCa                                                                              >  2:739065/1‑139 (MQ=255)                                                         aTAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCaa                                                                             >  2:33416/1‑139 (MQ=255)                                                             aCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     <  1:105118/95‑1 (MQ=255)                                                             aCATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     >  2:105118/1‑95 (MQ=255)                                                                    gACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                  <  1:33416/139‑1 (MQ=255)                                                                      cATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAg                                                                <  1:918714/139‑1 (MQ=255)                                                                           tatGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAAc                                                           <  2:392298/139‑1 (MQ=255)                                                                             tGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTa                                                         <  2:796483/139‑1 (MQ=255)                                                                                ctgctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCt                                                      >  2:968923/1‑139 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGt                                                                                                                          >  2:793558/1‑68 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGt                                                                                                                          <  1:793558/68‑1 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCaa                                                             >  1:410046/1‑129 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCaa                                                             <  2:410046/129‑1 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                                   >  1:1016328/1‑139 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                                   >  1:341710/1‑139 (MQ=255)                                                                                   ctgGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCtgtg                                                   >  2:148825/1‑139 (MQ=255)                                                                                         aGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        >  2:416396/1‑128 (MQ=255)                                                                                         aGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        <  1:416396/128‑1 (MQ=255)                                                                                           ccTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGACAAAATACATGACCGAg                                                                                                      >  2:712082/1‑80 (MQ=255)                                                                                           ccTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGACAAAATACATGACCGAg                                                                                                      <  1:712082/80‑1 (MQ=255)                                                                                              cccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                        <  1:260114/139‑1 (MQ=255)                                                                                              cccTGCCTCCATAAAAATGGACGTTCTTCTTCGTAGGGTGACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGa                                        <  2:97018/139‑1 (MQ=255)                                                                                               ccTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCATGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAg                                       <  1:381195/139‑1 (MQ=255)                                                                                                cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                  <  2:992559/111‑1 (MQ=255)                                                                                                cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                  >  1:992559/1‑111 (MQ=255)                                                                                                cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGt                                      >  2:1032877/1‑139 (MQ=255)                                                                                                cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGt                                      >  2:92842/1‑139 (MQ=255)                                                                                                cTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGt                                      <  2:393647/139‑1 (MQ=255)                                                                                                 tGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaata                                                                                   >  1:1070427/1‑93 (MQ=255)                                                                                                 tGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTtaata                                                                                   <  2:1070427/93‑1 (MQ=255)                                                                                                      ccATGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCAt                                <  2:1016328/139‑1 (MQ=255)                                                                                                         tGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTa                                                         <  2:45648/111‑1 (MQ=255)                                                                                                         tGAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTa                                                         >  1:45648/1‑111 (MQ=255)                                                                                                          gAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCa                            <  1:968923/139‑1 (MQ=255)                                                                                                          gAAAATAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCa                            <  2:28690/139‑1 (MQ=255)                                                                                                             aaTAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                  >  2:907875/1‑98 (MQ=255)                                                                                                             aaTAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCt                                                                  <  1:907875/98‑1 (MQ=255)                                                                                                             aaTAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGc                         <  2:341710/139‑1 (MQ=255)                                                                                                               tAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTgtgt                                                  >  2:283835/1‑112 (MQ=255)                                                                                                               tAGACGTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTgtgt                                                  <  1:283835/112‑1 (MQ=255)                                                                                                                    gTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgcg                                                                                                   <  2:913631/58‑1 (MQ=255)                                                                                                                    gTTCTTCTTCGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGgcg                                                                                                   >  1:913631/1‑58 (MQ=255)                                                                                                                     ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCt                                                                       >  2:535984/1‑85 (MQ=255)                                                                                                                     ttcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCt                                                                       <  1:535984/85‑1 (MQ=255)                                                                                                                      tcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     >  1:469966/1‑38 (MQ=255)                                                                                                                      tcttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCa                                                                                                                     <  2:469966/38‑1 (MQ=255)                                                                                                                       cttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAgg                                                                                                     <  2:714712/53‑1 (MQ=255)                                                                                                                       cttcttcGTAGGGTCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAgg                                                                                                     >  1:714712/1‑53 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               >  1:53522/1‑46 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCgcggc                                                                                               <  2:53522/46‑1 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        <  1:82574/85‑1 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTAt                                                        >  2:82574/1‑85 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATgg                                         >  1:323138/1‑100 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATgg                                         <  2:323138/100‑1 (MQ=255)                                                                                                                                    tCACGCTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGaa  >  1:969422/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      AGATGTTGTTTTCCGTTGTGCGATCCACGCCATCCCCAAGAACGGGGCTATACCAGATAAACATCGGGACATGATATGCCTGCTGGCTAGCCTCCCTGCCTCCATGAAAATAGACGTTCTTCTTCGTAGGGTCACGTTCCAGGCCGTGGTCGGCAAAATACATGACCGAGGCGCGGCGATCTTTTAATAATTCAAAAACCTGACCTAGCAAACTATCTGTGTAATGAATGGAGTTGTCATAGCAGGCATCCTGATCGTCCTGCGGTTGAA  >  NC_000913/3320197‑3320466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑