Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,121	C→T	*310* (TAG→TAA)	yhcC ←	putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,121	0	C	T	100.0%	171.3 / NA	53	*310* (TAG→TAA)	yhcC	putative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/29);  total (24/29)

AGGTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCG  >  NC_000913/3352985‑3353248                                                                                                                                         |                                                                                                                                aGGTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTa                                                                                                                               <  2:704651/139‑1 (MQ=255)   gTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACt                                                                                                                             <  1:278640/139‑1 (MQ=255)      aaCATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCg                                                                                                                          <  1:942543/139‑1 (MQ=255)           aataCTGCGCCAGCAGACGAATTTGCTTCATTA‑GGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    <  1:24730/139‑1 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTAc                                                                                                                              <  1:421308/123‑1 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTAc                                                                                                                              >  2:421308/1‑123 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  2:420188/1‑139 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  1:116534/1‑139 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  2:617499/1‑139 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  1:610795/1‑139 (MQ=255)                  cgcCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGcc                                                                                                              >  1:19021/1‑139 (MQ=255)                     cagcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACg                                                                                                           <  1:687127/139‑1 (MQ=255)                       gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  2:116534/139‑1 (MQ=255)                       gcagACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGa                                                                                                         <  1:420188/139‑1 (MQ=255)                                   gCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  2:324335/139‑1 (MQ=255)                                   gCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:1019759/139‑1 (MQ=255)                                           aGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTg                                                                                     <  2:318747/139‑1 (MQ=255)                                             ggAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                   >  2:765483/1‑107 (MQ=255)                                             ggAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAgg                                                                                                                   <  1:765483/107‑1 (MQ=255)                                                 ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    >  1:512415/1‑102 (MQ=255)                                                 ttCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAg                                                                                                                    <  2:512415/102‑1 (MQ=255)                                                  tCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:73276/1‑124 (MQ=255)                                                  tCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:73276/124‑1 (MQ=255)                                                    cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                    <  2:880424/131‑1 (MQ=255)                                                    cTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGt                                                                                    >  1:880424/1‑131 (MQ=255)                                                            cAACCTGCCCCAATAATACCCCATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACCCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  2:115376/114‑1 (MQ=255)                                                            cAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  1:115376/1‑114 (MQ=255)                                                               ccTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGc                                                                                                               >  1:392625/1‑93 (MQ=255)                                                               ccTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGc                                                                                                               <  2:392625/93‑1 (MQ=255)                                                                 tGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAg                                                               <  2:245981/139‑1 (MQ=255)                                                                    cccAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGa                                                            >  2:1047201/1‑139 (MQ=255)                                                                      cAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATc                                                          >  1:676728/1‑139 (MQ=255)                                                                                  aTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATa                                                                >  1:390486/1‑121 (MQ=255)                                                                                  aTTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATa                                                                <  2:390486/121‑1 (MQ=255)                                                                                       gACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCCCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  1:86380/90‑1 (MQ=255)                                                                                       gACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCCCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          >  2:86380/1‑90 (MQ=255)                                                                                             tACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATc                                   <  2:19021/139‑1 (MQ=255)                                                                                                       aTTGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGca                         <  2:1027703/139‑1 (MQ=255)                                                                                                        ttGTGTGAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcac                        <  1:1047201/139‑1 (MQ=255)                                                                                                          gtgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcacca                      <  1:557831/139‑1 (MQ=255)                                                                                                           tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          >  1:81189/1‑70 (MQ=255)                                                                                                           tgtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGAt                                                                                          <  2:81189/70‑1 (MQ=255)                                                                                                            gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGa                                                                                           <  2:358534/68‑1 (MQ=255)                                                                                                            gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGa                                                                                           >  1:358534/1‑68 (MQ=255)                                                                                                            gtgAGAAATGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGcaccacg                    >  2:466535/1‑138 (MQ=255)                                                                                                                  aaTGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGag              >  2:210221/1‑139 (MQ=255)                                                                                                                     gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTg                                                                                     <  2:397039/65‑1 (MQ=255)                                                                                                                     gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTg                                                                                     >  1:397039/1‑65 (MQ=255)                                                                                                                     gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaa           >  2:750896/1‑139 (MQ=255)                                                                                                                        gAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaagca        <  1:617499/139‑1 (MQ=255)                                                                                                                         aaTTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             >  2:57496/1‑53 (MQ=255)                                                                                                                         aaTTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGcc                                                                                             <  1:57496/53‑1 (MQ=255)                                                                                                                              cccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCATGGCGGAGCAAGCAGCGTCg  >  2:354020/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                AGGTCAACATAATACTGCGCCAGCAGACGAATTTGCTTCATTAGGGAATTCCTTCACGACAACCTGCCCCAATAATACCCAATTCTGACAGTTACGTTATAAATTGTGTGAGAAATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCG  >  NC_000913/3352985‑3353248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑