Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,353,230	A→G	L274P (CTG→CCG)	yhcC ←	putative Fe‑S oxidoreductase, Radical SAM superfamily protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,353,230	0	A	G	100.0%	139.4 / NA	40	L274P (CTG→CCG)	yhcC	putative Fe‑S oxidoreductase, Radical SAM superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/17);  total (23/17)

AATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGC  >  NC_000913/3353098‑3353363                                                                                                                                     |                                                                                                                                      aaTGCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGag                                                                                                                                 >  2:210221/1‑139 (MQ=255)    gCGGAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaa                                                                                                                              >  2:750896/1‑139 (MQ=255)       gAATTCCCCTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGagcaagca                                                                                                                           <  1:617499/139‑1 (MQ=255)             cccTCAACGACTTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCATGGCGGAGCAAGCAGCGTCg                                                                                                                     >  2:354020/1‑139 (MQ=255)                        ttACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGgcg                                                                                                          <  1:210221/138‑1 (MQ=255)                                       tAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCATGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                           <  1:354020/139‑1 (MQ=255)                                          ccACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCggtgg                                                                                        >  1:152594/1‑139 (MQ=255)                                          ccACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCggtgg                                                                                        >  2:345280/1‑139 (MQ=255)                                                aCGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGACAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATc                                                                                  <  2:676728/139‑1 (MQ=255)                                                 cGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCa                                                                                 >  1:81106/1‑139 (MQ=255)                                                    cccAGCGCCCATCCCCTTACGCCATGTTCATTCAGATAACGATCCCGCCCGACCATTCCCCTCCACCGATTTTCGCACCACGGCGGGGGAAGCAGCGTCGGGCGCCGGGGGCCGGCGGAAAAGGGGGGGTAGATCACtt                                                                              >  2:1057197/1‑139 (MQ=255)                                                       aGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCg                                                                           <  1:345280/139‑1 (MQ=255)                                                              aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGt                                                                    >  2:915211/1‑139 (MQ=255)                                                              aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGt                                                                    >  1:441385/1‑139 (MQ=255)                                                              aTCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGt                                                                    >  2:430922/1‑139 (MQ=255)                                                                   tGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                           <  2:941144/111‑1 (MQ=255)                                                                   tGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCgg                                                                                           >  1:941144/1‑111 (MQ=255)                                                                              ttcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                              >  1:452724/1‑97 (MQ=255)                                                                              ttcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATg                                                                                              <  2:452724/97‑1 (MQ=255)                                                                               tcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGaa                                                            <  2:227050/130‑1 (MQ=255)                                                                               tcattcaGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGaa                                                            >  1:227050/1‑130 (MQ=255)                                                                                        tAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGa                                          <  1:1057197/139‑1 (MQ=255)                                                                                           cGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCg                                       <  2:441385/139‑1 (MQ=255)                                                                                             aTCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCgtg                                     >  1:642823/1‑139 (MQ=255)                                                                                                cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCgg                                                                          <  1:126882/99‑1 (MQ=255)                                                                                                cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCgg                                                                          >  2:126882/1‑99 (MQ=255)                                                                                                cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTg                                                                        >  2:619567/1‑101 (MQ=255)                                                                                                cAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTg                                                                        <  1:619567/101‑1 (MQ=255)                                                                                                   cTCGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcc                               >  1:738553/1‑139 (MQ=255)                                                                                                     cGACCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAAtcctc                             <  1:787968/139‑1 (MQ=255)                                                                                                       aCCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGc                                               <  2:510386/119‑1 (MQ=255)                                                                                                       aCCATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGc                                               >  1:510386/1‑119 (MQ=255)                                                                                                        ccATTCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAg                          >  2:856183/1‑139 (MQ=255)                                                                                                            tCCCGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCa                      >  2:726587/1‑139 (MQ=255)                                                                                                               cGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATa                   <  2:119518/139‑1 (MQ=255)                                                                                                               cGTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATa                   <  2:81106/139‑1 (MQ=255)                                                                                                                gTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCggtg                                                                                         <  2:560001/68‑1 (MQ=255)                                                                                                                gTCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCggtg                                                                                         >  1:560001/1‑68 (MQ=255)                                                                                                                 tCCATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATAcc                 >  1:923352/1‑139 (MQ=255)                                                                                                                  ccATCGATTTTCGCACCACGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCg                >  2:184741/1‑139 (MQ=255)                                                                                                                                accacGGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAAACATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAGACGcccccc  >  2:634859/1‑137 (MQ=255)                                                                                                                                     |                                                                                                                                      AATGCGGAATTCCCCTCAACGACCTACTCCGTTGGAGGTAGCCACGGACGTCCCAGCGCCGATCCCTGTACGCCATGTTCATTCAGATAGCGATCCAGCTCGACCATTCCCGTCCATCGATTTTCGCACCACAGCGGAGCAAGCAGCGTCGGGCGACGGGCGCTGGCGGAAATGCGGTGGTAGATCACTTCCGGTGGCGTATGGCGAATCATTTCTCCGGCAGTGAGCGTGTAATCCTCCAGTTCAATACCGTTCAAACGCCCCGC  >  NC_000913/3353098‑3353363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑