Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,372,254	G→A	G107G (GGC→GGT)	nanT ←	sialic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,372,254	0	G	A	100.0%	139.8 / NA	44	G107G (GGC→GGT)	nanT	sialic acid transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (23/21);  total (23/21)

CTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGC  >  NC_000913/3372121‑3372388                                                                                                                                      |                                                                                                                                         cTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTgg                                                                                                                                     <  1:409065/139‑1 (MQ=255)   gAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGgcg                                                                                                                                   >  1:866054/1‑139 (MQ=255)     aaTCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCa                                                                                                                                 >  2:811370/1‑139 (MQ=255)        cAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAg                                                                                                                              <  2:783013/139‑1 (MQ=255)                        ttGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTcc                                                                                                              <  1:554268/139‑1 (MQ=255)                               gCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCga                                                                                                       >  2:195203/1‑139 (MQ=255)                                  gATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaa                                                                                                    <  2:700820/139‑1 (MQ=255)                                   aTGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaag                                                                                                   >  2:428102/1‑139 (MQ=255)                                     gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                <  2:566580/108‑1 (MQ=255)                                     gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                >  1:566580/1‑108 (MQ=255)                                     gTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCgagaagag                                                                                                 >  2:238531/1‑139 (MQ=255)                                             cAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGc                                                                                         >  2:488535/1‑139 (MQ=255)                                                    cAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGAc                                                                                <  1:238531/139‑1 (MQ=255)                                                          cATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGc                                                                          >  1:657657/1‑139 (MQ=255)                                                             aGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAg                                                                       >  2:702426/1‑139 (MQ=255)                                                             aGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAg                                                                       >  2:677097/1‑139 (MQ=255)                                                               gTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAgac                                                                     <  2:866054/139‑1 (MQ=255)                                                                   cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGacgacg                                                                 >  1:90892/1‑139 (MQ=255)                                                                   cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGacgacg                                                                 <  1:811370/139‑1 (MQ=255)                                                                   cgcTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGacgacg                                                                 >  1:488985/1‑139 (MQ=255)                                                                              tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCg                                                        <  2:133243/137‑1 (MQ=255)                                                                              tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCg                                                        >  1:133243/1‑137 (MQ=255)                                                                              tATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGt                                                      <  1:677097/139‑1 (MQ=255)                                                                                   aCCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                >  1:845850/1‑62 (MQ=255)                                                                                   aCCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                <  2:845850/62‑1 (MQ=255)                                                                                     ccGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCAt                                               >  2:24127/1‑139 (MQ=255)                                                                                      cGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATa                                              <  2:488985/139‑1 (MQ=255)                                                                                            ccccATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCg                                        <  2:657657/139‑1 (MQ=255)                                                                                               cATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGTCGCaa                                                                                                                                >  2:974033/1‑50 (MQ=255)                                                                                               cATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCaa                                                                                                                                <  1:974033/50‑1 (MQ=255)                                                                                                      aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAg                                                          <  2:75442/111‑1 (MQ=255)                                                                                                      aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAg                                                          >  1:75442/1‑111 (MQ=255)                                                                                                      aTGACCAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGgc                              >  2:917699/1‑139 (MQ=255)                                                                                                           cAGACGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGccgccg                         <  2:90892/139‑1 (MQ=255)                                                                                                              aCGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAc                      >  2:594781/1‑139 (MQ=255)                                                                                                              aCGAGCGATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAAc                      >  2:289628/1‑139 (MQ=255)                                                                                                                    gATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCga                <  1:488535/139‑1 (MQ=255)                                                                                                                    gATAAACATGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCga                <  1:428102/139‑1 (MQ=255)                                                                                                                            tGGTGATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATaaa        <  2:416388/139‑1 (MQ=255)                                                                                                                               taaTGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAGGAGAATGATCCTGGTGTGACCATTGCCAGCCGAGGCCCGTAGCGGTCACCCATAGCGGCGGGCATCAGGCGGCAGAACCCGCGAGATATACAg       >  2:299418/3‑139 (MQ=255)                                                                                                                                gATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTc                                                     <  1:127802/90‑1 (MQ=255)                                                                                                                                gATGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTc                                                     >  2:127802/1‑90 (MQ=255)                                                                                                                                  tGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc  <  1:824663/139‑1 (MQ=255)                                                                                                                                  tGTAACCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGc  >  2:824663/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                         CTGAAATCAAAAAACCACTGGCTTTGTTACGCAGATGTTTTGGCCAGCTTTCAATGACATAGGTGGCGCTGGAACCGTATTCACCCGCCATCCCCATGCCGATGACCAGACGAGCGATAAACATGGTGATGTAGCCTGGCGCAAAGCCGCAGGCCAGCGTCCCGGCCGAGAAGAGAACGATGCTG‑‑GTGACCATTGCCAGACGACGCCCGTAGCGGTCACCCATAGCGCCGAGCATCAGGCCGCCGAACCAGCGAGAGATAAAGGCTGC  >  NC_000913/3372121‑3372388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑