Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,398,875 C→T M1M (GTG→ATG) †
*368* (TAG→TAA
mreD ←
mreC ←
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,398,8750CT100.0% 76.0 / NA 25M1M (GTG→ATG) †
*368* (TAG→TAA
mreD
mreC
cell wall structural complex MreBCD transmembrane component MreD
cell wall structural complex MreBCD transmembrane component MreC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (14/11);  total (14/11)

GAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGC  >  NC_000913/3398753‑3398988
                                                                                                                          |                                                                                                                 
gAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTACCCTCCCGgcg                                                                                                   >  1:250143/1‑139 (MQ=255)
        cccAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCa                                                                                           >  1:691465/1‑139 (MQ=255)
                                ggTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggc                                                                   >  2:1011572/1‑139 (MQ=255)
                                    aTCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcggcggcg                                                               <  1:977536/139‑1 (MQ=255)
                                         gCCAGGGCATGATTTGCAGAAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGcc                                                          >  1:716133/1‑139 (MQ=255)
                                                   gATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                      >  1:1013121/1‑101 (MQ=255)
                                                   gATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                      <  2:1013121/101‑1 (MQ=255)
                                                               cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCAc                                                                                                <  2:994818/79‑1 (MQ=255)
                                                               cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCAc                                                                                                >  1:994818/1‑79 (MQ=255)
                                                               cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg                                                                    >  2:885551/1‑107 (MQ=255)
                                                               cAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTgcgg                                                                    <  1:885551/107‑1 (MQ=255)
                                                                  cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                      <  2:286904/86‑1 (MQ=255)
                                                                  cgcAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCgg                                                                                      >  1:286904/1‑86 (MQ=255)
                                                                    cAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGACATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGc                               <  2:716133/139‑1 (MQ=255)
                                                                                 gagCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCCTTGTGGAGAGCGATTAGCAGCAGGc                               >  2:739472/1‑126 (MQ=255)
                                                                                 gagCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCCTTGTGGAGAGCGATTAGCAGCAGGc                               <  1:739472/126‑1 (MQ=255)
                                                                                    ccAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGcagcag               >  2:566431/1‑139 (MQ=255)
                                                                                      aGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATAATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGgc             <  2:685425/139‑1 (MQ=255)
                                                                                       gATTACCCAGCGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGgcg            <  1:1011572/139‑1 (MQ=255)
                                                                                                 cGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc  >  1:906562/1‑139 (MQ=255)
                                                                                                 cGTCCCTGGCTACGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGc  >  1:492132/1‑139 (MQ=255)
                                                                                                             cGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTa       <  1:384433/122‑1 (MQ=255)
                                                                                                             cGATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTa       >  2:384433/1‑122 (MQ=255)
                                                                                                              gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt                                          >  2:4128/1‑86 (MQ=255)
                                                                                                              gATAGCTCGCCATTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGAt                                          <  1:4128/86‑1 (MQ=255)
                                                                                                                          |                                                                                                                 
GAGTAACACCCAGTTTGGCCGGAAAACAATCAGGTTATCCGGCCAGGGCATGATTTGCAGCAACAGCGCAATGAGGAAAGAGAGCCAGATTACCCAGCGTCCCTGGCTACGATAGCTCGCCACTATTGCCCTCCCGGCGCACGCGCAGGCGGTTGAGCACCACTTTGCGGCGGCGTAGCCCTTTGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGC  >  NC_000913/3398753‑3398988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: