Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,414,786	C→T	P308L (CCG→CTG)	acrE →	cytoplasmic membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,414,786	0	C	T	100.0%	158.5 / NA	49	P308L (CCG→CTG)	acrE	cytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/22);  total (27/22)

ATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGG  >  NC_000913/3414651‑3414901                                                                                                                                        |                                                                                                                    aTGTGGCCGCTGATGATAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTTCCGGATATGTTTGTCCGTGCGCGGATTGATGAGGGCGTCGAACGTGACGCCATTCTTATCCTGca                                                                                                                  >  2:23759/1‑139 (MQ=255)      aCCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAg                                                                                                             <  1:242607/139‑1 (MQ=255)                                aaCCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGAt                                                                                   <  2:360669/139‑1 (MQ=255)                                    cTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCaa                                                                               >  1:1065842/1‑139 (MQ=255)                                    cTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCaa                                                                               <  1:903116/139‑1 (MQ=255)                                     tACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                                                              >  2:768802/1‑139 (MQ=255)                                     tACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAAc                                                                              <  1:197968/139‑1 (MQ=255)                                       cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACcg                                                                                            >  1:1013939/1‑123 (MQ=255)                                       cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACcg                                                                                            <  2:1013939/123‑1 (MQ=255)                                       cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCg                                                                            >  1:336742/1‑139 (MQ=255)                                       cGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCg                                                                            >  1:829485/1‑139 (MQ=255)                                         tGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTg                                                                          <  2:715464/139‑1 (MQ=255)                                           cTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGTGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCt                                                                        >  1:421473/1‑139 (MQ=255)                                            tGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTg                                                                       >  1:848305/1‑139 (MQ=255)                                             gTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGca                                                                                                 >  1:360048/1‑112 (MQ=255)                                             gTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGca                                                                                                 <  2:360048/112‑1 (MQ=255)                                             gTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGa                                                                      >  2:649459/1‑139 (MQ=255)                                                  cccTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGtt                                                                 <  2:1061370/139‑1 (MQ=255)                                                            cAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATaaaa                                                       >  1:633715/1‑139 (MQ=255)                                                            cAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATaaaa                                                       >  1:226355/1‑139 (MQ=255)                                                                         tGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAgc                                          >  1:1008890/1‑139 (MQ=255)                                                                              ggTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     <  1:23759/139‑1 (MQ=255)                                                                                       gtgcgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTg                            <  2:336742/139‑1 (MQ=255)                                                                                          cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     <  1:887449/127‑1 (MQ=255)                                                                                          cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     >  2:887449/1‑127 (MQ=255)                                                                                          cgtgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCa                         >  1:20606/1‑139 (MQ=255)                                                                                            tgcACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGt                       >  2:583150/1‑139 (MQ=255)                                                                                              cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                         >  1:982483/1‑55 (MQ=255)                                                                                              cACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGt                                                                                                         <  2:982483/55‑1 (MQ=255)                                                                                                  gATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACcgc                                                                                           <  1:661637/65‑1 (MQ=255)                                                                                                  gATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACcgc                                                                                           >  2:661637/1‑65 (MQ=255)                                                                                                       atgaAGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTgg            >  1:80795/1‑139 (MQ=255)                                                                                                           aGGCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGAt        <  1:768802/139‑1 (MQ=255)                                                                                                             gCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     <  1:177576/108‑1 (MQ=255)                                                                                                             gCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     >  2:177576/1‑108 (MQ=255)                                                                                                             gCGTCCAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATaa      >  2:493936/1‑139 (MQ=255)                                                                                                                 ccAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGAt                                                           >  1:195422/1‑82 (MQ=255)                                                                                                                 ccAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGAt                                                           <  2:195422/82‑1 (MQ=255)                                                                                                                  cAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGc                  <  1:545740/122‑1 (MQ=255)                                                                                                                  cAACCTGACGCCATTCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGc                  >  2:545740/1‑122 (MQ=255)                                                                                                                               ttCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGc                           >  1:63784/1‑100 (MQ=255)                                                                                                                               ttCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGc                           <  2:63784/100‑1 (MQ=255)                                                                                                                               ttCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATgg  <  2:1059355/125‑1 (MQ=255)                                                                                                                               ttCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATgg  >  1:1059355/1‑125 (MQ=255)                                                                                                                                tCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     >  1:326290/1‑89 (MQ=255)                                                                                                                                tCTTATCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGcc                                     <  2:326290/89‑1 (MQ=255)                                                                                                                                    aTCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGt                       <  2:515763/99‑1 (MQ=255)                                                                                                                                    aTCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGt                       >  1:515763/1‑99 (MQ=255)                                                                                                                                    aTCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGa                >  2:65174/1‑106 (MQ=255)                                                                                                                                    aTCCTGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGa                <  1:65174/106‑1 (MQ=255)                                                                                                                                        |                                                                                                                    ATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGATGAAGGCGTCCAACCTGACGCCATTCTTATCCCGCAACAAGGCGTTAGCCGCACACCGCGTGGTGATGCAACCGTGCTGATTGTTAACGATAAAAGTCAGGTTGAAGCGCGCCCTGTCGTTGCCAGTCAGGCGATTGGCGATAAATGG  >  NC_000913/3414651‑3414901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑