Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,547,986 C→T *695* (TAG→TAA)  malQ ← 4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,547,9860CT98.0% 153.0 / ‑4.3 51*695* (TAG→TAA) malQ4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (25/25);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

TCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGC  >  NC_000913/3547851‑3548119
                                                                                                                                       |                                                                                                                                     
tCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTAc                                                                                                                                    >  1:196748/1‑139 (MQ=255)
  tgctgctgAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActt                                                                                                                                  >  2:1039648/1‑139 (MQ=255)
        tgAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTActtcttctt                                                                                                                            <  1:1039648/139‑1 (MQ=255)
                 tGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGc                                                                                                                   >  2:914404/1‑139 (MQ=255)
                 tGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGc                                                                                                                   <  1:914404/139‑1 (MQ=255)
                          aaCACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCg                                                                                                          >  1:912726/1‑139 (MQ=255)
                                                gTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAAc                                                                                    <  2:1028899/139‑1 (MQ=255)
                                                         ttATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACg                                                                           <  2:176926/139‑1 (MQ=255)
                                                           aTCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGTATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGcc                                                                         <  1:496207/139‑1 (MQ=255)
                                                                 ccTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCatcatc                                                                   >  1:125551/1‑139 (MQ=255)
                                                                  cTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGctc                                                                                                                 >  2:529493/1‑92 (MQ=255)
                                                                  cTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGctc                                                                                                                 <  1:529493/92‑1 (MQ=255)
                                                                    aCGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGc                                                                <  2:912726/139‑1 (MQ=255)
                                                                     cGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCa                                                               >  2:971899/1‑139 (MQ=255)
                                                                     cGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCa                                                               <  2:125551/139‑1 (MQ=255)
                                                                           gggATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATc                                                         >  2:166165/1‑139 (MQ=255)
                                                                                 aaTTAGTAGGCCGGATAAGACGCGTCAAGCATCACATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCa                                                                                                  <  1:156796/92‑1 (MQ=255)
                                                                                 aaTTAGTAGGCCGGATAAGACGCGTCAAGCATCACATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCa                                                                                                  >  2:156796/1‑92 (MQ=255)
                                                                                        agGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTg                                            >  1:440748/1‑139 (MQ=255)
                                                                                          gCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCg                                          >  1:626879/1‑139 (MQ=255)
                                                                                           ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                         <  2:398219/75‑1 (MQ=255)
                                                                                           ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGt                                                                                                         >  1:398219/1‑75 (MQ=255)
                                                                                           ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcat                                                                       >  1:700133/1‑109 (MQ=255)
                                                                                           ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCcat                                                                       <  2:700133/109‑1 (MQ=255)
                                                                                           ccGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCgg                                         >  1:758668/1‑139 (MQ=255)
                                                                                            cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGa                                        >  1:691053/1‑139 (MQ=255)
                                                                                            cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGa                                        >  1:21746/1‑139 (MQ=255)
                                                                                            cGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGa                                        >  2:585589/1‑139 (MQ=255)
                                                                                                      gcgTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGTGGAAAGCTTGCGt                              <  2:196748/139‑1 (MQ=255)
                                                                                                        gTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCaa                                                              <  2:834582/105‑1 (MQ=255)
                                                                                                        gTCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCaa                                                              >  1:834582/1‑105 (MQ=255)
                                                                                                         tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                       <  1:833613/47‑1 (MQ=255)
                                                                                                         tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTg                                                                                                                       >  2:833613/1‑47 (MQ=255)
                                                                                                         tCAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                           <  1:274295/139‑1 (MQ=255)
                                                                                                         tCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGc                           <  2:21746/139‑1 (MQ=255)
                                                                                                          cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  1:971899/139‑1 (MQ=255)
                                                                                                          cAAGCATCGCATCCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGcc                          <  2:440748/139‑1 (MQ=255)
                                                                                                                tcgcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCaaa                                                                                                <  1:656213/63‑1 (MQ=255)
                                                                                                                tcgcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCaaa                                                                                                >  2:656213/1‑63 (MQ=255)
                                                                                                                  gcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTta                  >  2:144408/1‑139 (MQ=255)
                                                                                                                  gcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTta                  >  2:965962/1‑139 (MQ=255)
                                                                                                                  gcatcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCATGCTGTTCAGATCCTTCAGCAACATGTTCACGCCATTATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTta                  <  2:334270/139‑1 (MQ=255)
                                                                                                                   catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGc                                                                                       <  1:760134/69‑1 (MQ=255)
                                                                                                                   catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACAt                                                          >  1:532290/1‑98 (MQ=255)
                                                                                                                   catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACAt                                                          <  2:532290/98‑1 (MQ=255)
                                                                                                                   catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTtat                 <  1:585589/139‑1 (MQ=255)
                                                                                                                   catcCGGCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTCCGCCGTCTGTCCAAATCCTTCAGc                                                                                       >  2:760134/1‑69 (MQ=255)
                                                                                                                        ggCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                         <  1:242921/62‑1 (MQ=255)
                                                                                                                        ggCATCAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCa                                                                                         >  2:242921/1‑62 (MQ=255)
                                                                                                                             cAACCGCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACt       <  1:965962/139‑1 (MQ=255)
                                                                                                                                  gCACTTTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGc  <  1:144408/139‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
TCTGCTGCTGAATATGGTGATTTGATAACACTGACTGCCGGATGCGGCGTGACCGCCTTATCCGGCCTACGATTCGGGATGAATTAGTAGGCCGGATAAGACGCGTCAAGCATCGCATCCGGCATCAACCGCACTCTACTTCTTCTTCGCTGCAGCTCTGCGCCGTCTGTCCAAATCCTTCAGCAACTTGTTCACGCCATCATCGGCAAACATCGACTCAAGCGTTGCGGAAAGCTTGCGTCGCCAGTTTTTATACTGGTAACTGGTGC  >  NC_000913/3547851‑3548119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑