Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,731,980	G→A	G284S (GGT→AGT)	xylF →	D‑xylose transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,731,980	0	G	A	100.0%	225.9 / NA	69	G284S (GGT→AGT)	xylF	D‑xylose transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (32/37);  total (32/37)

AGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAG  >  NC_000913/3731847‑3732109                                                                                                                                      |                                                                                                                                  aGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                              <  1:774853/139‑1 (MQ=255) aGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAg                                                                                                                              <  2:841166/139‑1 (MQ=255)       ctcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                        <  1:724595/139‑1 (MQ=255)        tcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                       >  2:405126/1‑139 (MQ=255)        tcCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                       <  2:692710/139‑1 (MQ=255)          cGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaaaa                                                                                                                     >  2:756349/1‑139 (MQ=255)            gCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGc                                                                                                                   <  1:569624/139‑1 (MQ=255)                 gATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                              <  1:756349/139‑1 (MQ=255)                 gATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                              <  1:405126/139‑1 (MQ=255)                                  tATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGc                                                                                             >  2:199941/1‑139 (MQ=255)                                  tATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGc                                                                                             >  2:361857/1‑139 (MQ=255)                                     tAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTg                                                                                          <  1:942836/139‑1 (MQ=255)                                            aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                       >  2:829983/1‑119 (MQ=255)                                            aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACt                                                                                                       <  1:829983/119‑1 (MQ=255)                                            aTTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATg                                                                                   >  2:883910/1‑139 (MQ=255)                                              tgctgcCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTc                                                                                 >  2:360559/1‑139 (MQ=255)                                              tgctgcCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTc                                                                                 >  2:1002549/1‑139 (MQ=255)                                                      gTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                         <  1:361857/139‑1 (MQ=255)                                                      gTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCg                                                                         <  2:888184/139‑1 (MQ=255)                                                       tACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGc                                                                        <  1:883910/139‑1 (MQ=255)                                                       tACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGc                                                                        <  2:1060580/139‑1 (MQ=255)                                                            aaaCTATGACTCTGTATACACCTATTACGTTGTTGGCAAATACTGCATCAGAAACTCCCGTTGCGTTGGGCAACACTCCCGAATCAAAAGCATCTACCACACCTAACAATGCCCTGAAACAGGTCCCCTGCCGgctact                                                                   <  2:407470/139‑1 (MQ=255)                                                                      ggTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatc                                                         <  1:199941/139‑1 (MQ=255)                                                                        tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                        >  1:371450/1‑74 (MQ=255)                                                                        tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCa                                                                                                                        <  2:371450/74‑1 (MQ=255)                                                                        tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                              >  2:225102/1‑84 (MQ=255)                                                                        tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                              <  1:225102/84‑1 (MQ=255)                                                                        tgtATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACcgatcga                                                       >  2:680108/1‑139 (MQ=255)                                                                                  tATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGACCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaa                                             <  1:288797/139‑1 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                       >  2:1071743/1‑63 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCaa                                                                                                                       <  1:1071743/63‑1 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTcc                                                                           >  2:510943/1‑107 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTcc                                                                           <  1:510943/107‑1 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGcctc                                                                     <  2:525456/113‑1 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGcctc                                                                     >  1:525456/1‑113 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                     >  2:547053/1‑129 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                     <  1:547053/129‑1 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                           >  1:793485/1‑139 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                           >  1:598402/1‑139 (MQ=255)                                                                                    ttACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATaaaaa                                           >  1:562473/1‑139 (MQ=255)                                                                                          tgttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACAt                                     <  1:1002549/139‑1 (MQ=255)                                                                                          tgttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACAt                                     <  1:360559/139‑1 (MQ=255)                                                                                          tgttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACAt                                     <  2:598402/139‑1 (MQ=255)                                                                                           gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                           <  1:113155/68‑1 (MQ=255)                                                                                           gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACca                                                                                                           >  2:113155/1‑68 (MQ=255)                                                                                           gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                     >  1:913124/1‑122 (MQ=255)                                                                                           gttgGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                     <  2:913124/122‑1 (MQ=255)                                                                                              ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGcc                                                                                            >  1:451815/1‑80 (MQ=255)                                                                                              ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGcc                                                                                            <  2:451815/80‑1 (MQ=255)                                                                                              ggCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCaaa                                 >  2:318767/1‑139 (MQ=255)                                                                                                cAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGa                               <  1:680108/139‑1 (MQ=255)                                                                                                   aTACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATc                                    <  2:995600/131‑1 (MQ=255)                                                                                                   aTACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATc                                    >  1:995600/1‑131 (MQ=255)                                                                                                      cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAAt                                                <  1:893419/116‑1 (MQ=255)                                                                                                      cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAAt                                                >  2:893419/1‑116 (MQ=255)                                                                                                      cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaaca                                         >  2:135806/1‑123 (MQ=255)                                                                                                      cTGCCGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAaaca                                         <  1:135806/123‑1 (MQ=255)                                                                                                         ccGCAGAAATTGCTGTTGAGTTGGGCAATAGTCATGAACCAAAAGCAGATACCACACTGaata                                                                                                  <  2:694993/63‑1 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                >  2:560154/1‑49 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGa                                                                                                                <  1:560154/49‑1 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGaata                                                                                                  >  1:694993/1‑63 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                     >  2:620644/1‑108 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGAtg                                                     <  1:620644/108‑1 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAAt                      >  2:403328/1‑139 (MQ=255)                                                                                                         ccGCAGAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAAt                      >  2:883268/1‑139 (MQ=255)                                                                                                              gAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                              <  1:69940/46‑1 (MQ=255)                                                                                                              gAAATTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATa                                                                                                              >  2:69940/1‑46 (MQ=255)                                                                                                                aaTTGCCGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGAc               <  2:793485/139‑1 (MQ=255)                                                                                                                      cGTTGAGTTGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTc         <  1:403328/139‑1 (MQ=255)                                                                                                                             ttGGGCAATAGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGGGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGAATCCACAAag  >  2:688959/1‑139 (MQ=255)                                                                                                                                      |                                                                                                                                  AGCAATCTCCGGCCAGGATGCGGATCTCGCAGGTATTAAACGTATTGCTGCCGGTACGCAAACTATGACGGTGTATAAACCTATTACGTTGTTGGCAAATACTGCCGCAGAAATTGCCGTTGAGTTGGGCAATGGTCAGGAACCAAAAGCAGATACCACACTGAATAATGGCCTGAAAGATGTCCCCTCCCGCCTCCTGACACCGATCGATGTGAATAAAAACAACATCAAAGATACGGTAATTAAAGACGGATTCCACAAAG  >  NC_000913/3731847‑3732109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑