Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,773,602 T→C V441A (GTT→GCT)  mtlA → mannitol‑specific PTS enzyme: IIA, IIB and IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,773,6020TC100.0% 92.9 / NA 28V441A (GTT→GCT) mtlAmannitol‑specific PTS enzyme: IIA, IIB and IIC components
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/12);  total (16/12)

GATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGA  >  NC_000913/3773490‑3773731
                                                                                                                |                                                                                                                                 
gATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGca                                                                                                                         <  1:229497/123‑1 (MQ=255)
gATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGca                                                                                                                         >  2:229497/1‑123 (MQ=255)
          tGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCt                                                                                               >  2:236950/1‑139 (MQ=255)
                 gATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGc                                                                                        <  1:236950/139‑1 (MQ=255)
                      cTGTTACTAACAGCGCGATCAACAACCTGCCCCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCCCAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                   >  2:796988/1‑139 (MQ=255)
                                         caacaaCCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGaa                                                                <  1:796988/139‑1 (MQ=255)
                                                                aCCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGc                                                                                                                              >  1:2413/1‑54 (MQ=255)
                                                                aCCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGc                                                                                                                              <  2:2413/54‑1 (MQ=255)
                                                                    cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACg                                     >  2:984538/1‑139 (MQ=255)
                                                                    cGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACg                                     >  2:788234/1‑139 (MQ=255)
                                                                     gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                   >  2:992323/1‑92 (MQ=255)
                                                                     gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                   >  2:527678/1‑92 (MQ=255)
                                                                     gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                   <  1:992323/92‑1 (MQ=255)
                                                                     gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCt                                                                                   <  1:527678/92‑1 (MQ=255)
                                                                     gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa                                              >  1:919567/1‑129 (MQ=255)
                                                                     gTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCaa                                              <  2:919567/129‑1 (MQ=255)
                                                                                 cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCca                                         >  1:796733/1‑122 (MQ=255)
                                                                                 cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCca                                         <  2:796733/122‑1 (MQ=255)
                                                                                 cGTGACCTGACCGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAgaga                        >  2:805727/1‑139 (MQ=255)
                                                                                   tgacctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGCACAGTATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCCCACGGCAAACGAAGAGaaa                      >  2:889604/1‑139 (MQ=255)
                                                                                     acctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGc                                                                                                                          <  2:371807/37‑1 (MQ=255)
                                                                                     acctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGc                                                                                                                          >  1:371807/1‑37 (MQ=255)
                                                                                       ctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                                       <  2:129348/38‑1 (MQ=255)
                                                                                       ctgaccGAACGCGCTATGCGCCAGGCTCCGCAGGcaca                                                                                                                       >  1:129348/1‑38 (MQ=255)
                                                                                              aaCGCGCTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAg           >  1:569339/1‑139 (MQ=255)
                                                                                                    cTATGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGaa     >  2:416286/1‑139 (MQ=255)
                                                                                                       tGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGa  <  2:569339/139‑1 (MQ=255)
                                                                                                       tGCGCCAGGCTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGa  <  1:788234/139‑1 (MQ=255)
                                                                                                                |                                                                                                                                 
GATGCAGGTCTGTCGCAGATTTCTGTTACTAACAGCGCGATCAACAACCTGCCGCCAGATGTGGACCTCGTCATCACTCACCGTGACCTGACCGAACGCGCTATGCGCCAGGTTCCGCAGGCACAGCATATTTCGCTGACCAACTTCCTCGACAGCGGCCTGTACACCAGCCTGACCGAACGTCTGGTTGCTGCCCAACGCCACACGGCAAACGAAGAGAAAGTAAAAGACAGCCTGAAAGA  >  NC_000913/3773490‑3773731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: