Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,789,047	C→T	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ → waaH ←	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,789,047	0	C	T	100.0%	148.7 / NA	46	A316V (GCT→GTT)  *345* (TAG→TAA)	yibQ waaH	putative polysaccharide deacetylase LPS(HepIII)‑glucuronic acid glycosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/23);  total (23/23)

CCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCG  >  NC_000913/3788914‑3789178                                                                                                                                      |                                                                                                                                    ccGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAgcg                                                                                                                                <  2:497623/139‑1 (MQ=255)   caTAGAACCTGTTTATGCTAATCGCTGCTTCGAACTATTAAGGCAAAGAATCACACAGATGACGCTGATCGTTTACTTCAAGCATCAGTGTCAAGACTCGGGCAAACACCACGAATCGGTGCAGTATAACGTTAGCGCa                                                                                                                              >  2:111339/2‑139 (MQ=255)               ttATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcgc                                                                                                                  <  1:293671/139‑1 (MQ=255)                 aTGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTa                                                                                                                <  1:1058934/139‑1 (MQ=255)                   gCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                              >  1:456419/1‑139 (MQ=255)                      aaTCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGt                                                                                                           >  2:394312/1‑139 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                         >  2:273159/1‑139 (MQ=255)                                        ttAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACcg                                                                                         >  1:287979/1‑139 (MQ=255)                                                aaGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGt                                                                                 >  1:332963/1‑139 (MQ=255)                                                aaGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGt                                                                                 >  1:560665/1‑139 (MQ=255)                                                aaGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGt                                                                                 >  1:1005114/1‑139 (MQ=255)                                                aaGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGt                                                                                 >  2:34297/1‑139 (MQ=255)                                                             agCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCa                                                                    >  1:867151/1‑139 (MQ=255)                                                               cACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                  <  2:679866/139‑1 (MQ=255)                                                               cACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATa                                                                  <  2:867151/139‑1 (MQ=255)                                                                 cGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAg                                                                >  1:724553/1‑139 (MQ=255)                                                                  gCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgcg                                                                                                            <  1:418235/94‑1 (MQ=255)                                                                  gCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcgcg                                                                                                            >  2:418235/1‑94 (MQ=255)                                                                       tCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGaaa                                                          <  2:332963/139‑1 (MQ=255)                                                                        cGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAg                                                         <  2:456419/139‑1 (MQ=255)                                                                            tACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                       >  1:50133/1‑137 (MQ=255)                                                                            tACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGAc                                                       <  2:50133/137‑1 (MQ=255)                                                                            tACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACca                                                     <  1:34297/139‑1 (MQ=255)                                                                                   aGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAAc                                              >  1:874672/1‑139 (MQ=255)                                                                                   aGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAAc                                              >  2:792650/1‑139 (MQ=255)                                                                                    gCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACg                                             <  1:273159/139‑1 (MQ=255)                                                                                        cAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                              <  1:981339/70‑1 (MQ=255)                                                                                        cAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTAcg                                                                                                              >  2:981339/1‑70 (MQ=255)                                                                                         aGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTgg                                        >  2:515286/1‑139 (MQ=255)                                                                                           tGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTa                                      >  1:103514/1‑139 (MQ=255)                                                                                             gCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAAc                                    >  1:230810/1‑139 (MQ=255)                                                                                              cAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTatcc                                   <  2:508208/139‑4 (MQ=255)                                                                                              cAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAAcc                                   <  2:103514/139‑1 (MQ=255)                                                                                               aaGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCg                                  >  2:1076501/1‑139 (MQ=255)                                                                                                aGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGa                                 <  1:515286/139‑1 (MQ=255)                                                                                                      gggCAAACAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCa                           >  1:884518/1‑139 (MQ=255)                                                                                                            aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcgc                                                                                                                  <  1:703984/46‑1 (MQ=255)                                                                                                            aCAGCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGgcgc                                                                                                                  >  2:703984/1‑46 (MQ=255)                                                                                                               gCCCGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGca                  <  2:724553/139‑1 (MQ=255)                                                                                                                cccGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGcac                 >  1:17492/1‑139 (MQ=255)                                                                                                                  cGAAGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACAt               <  2:17492/139‑1 (MQ=255)                                                                                                                    aaGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgcg                    <  2:876856/132‑1 (MQ=255)                                                                                                                    aaGCGGCGAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTgcg                    >  1:876856/1‑132 (MQ=255)                                                                                                                           gAAGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCa      <  1:1076501/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg    <  1:228888/139‑1 (MQ=255)                                                                                                                             aGTTTAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAgg    <  2:874672/139‑1 (MQ=255)                                                                                                                               tttAACGTTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGgcg  <  2:884518/139‑1 (MQ=255)                                                                                                                                      |                                                                                                                                    CCGATAGAACCTGTTTATGCTAATCGCTTCTTTGAAGTATTAAGCGAAAGCATCAGCCAGAGCACGCTGATCGTTTACTTCCAGCATCAGTGGCAAGGCTGGGGCAAACAGCCCGAAGCGGCGAAGTTTAACGCTAGCGCAAATTAAAGGCGCTACGCGTAATGCGATGGTGCGACCGCGTTTTGTCGCGCCATTGCCATAAGCGGAAAGACCACAGTAACGCCTGGTAACCGACCTTCACGCTGCGCACATTGGTAATCAGGCG  >  NC_000913/3788914‑3789178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑