Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	354,592	G→A	*629* (TAG→TAA)	prpE →	propionate‑‑CoA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,592	0	G	A	100.0%	147.5 / NA	46	*629* (TAG→TAA)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (26/20);  total (26/20)

TCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NC_000913/354473‑354726                                                                                                                        |                                                                                                                                       tCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTcg                                                                                                                     <  1:206752/139‑1 (MQ=255)                           ccAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCg                                                                                           <  2:948929/139‑1 (MQ=255)                            cAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGa                                                                                          <  2:739418/139‑1 (MQ=255)                               gCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACt                                                                                                   <  2:266008/127‑1 (MQ=255)                               gCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACt                                                                                                   >  1:266008/1‑127 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               <  2:404303/110‑1 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGt                                                                                                               >  1:404303/1‑110 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCgg                                                                                                       >  2:337527/1‑118 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCgg                                                                                                       <  1:337527/118‑1 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGAt                                                                                         >  1:1028781/1‑132 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGAt                                                                                         <  2:1028781/132‑1 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:956119/1‑139 (MQ=255)                                    ttGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGAt                                                                                  >  2:436360/1‑139 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     <  2:406499/95‑1 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCa                                                                                                     >  1:406499/1‑95 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCTCTGTGTGCCGATGCCTGGTGCGACGCTGACGCGTTTTATCATGc                                                         >  2:476192/1‑139 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        <  2:757047/124‑1 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGa                                                                        >  1:757047/1‑124 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc                                                         >  2:605535/1‑139 (MQ=255)                                                             cTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCAGCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGc                                                         >  1:681638/1‑139 (MQ=255)                                                                     cATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       <  1:847736/117‑1 (MQ=255)                                                                     cATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       >  2:474060/1‑117 (MQ=255)                                                                     cATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       <  1:474060/117‑1 (MQ=255)                                                                     cATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAc                                                                       >  2:847736/1‑117 (MQ=255)                                                                          atgatCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACt                                                                                                   <  2:176117/84‑1 (MQ=255)                                                                          atgatCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACt                                                                                                   >  1:176117/1‑84 (MQ=255)                                                                              tCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGt                                        >  2:956564/1‑139 (MQ=255)                                                                               ccTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTa                                       >  2:596424/1‑139 (MQ=255)                                                                                  gCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGAt                                                                                         >  2:334952/1‑86 (MQ=255)                                                                                  gCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGAt                                                                                         <  1:334952/86‑1 (MQ=255)                                                                                   cgtcgtTGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTc                                   >  2:1070672/1‑139 (MQ=255)                                                                                        ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATa                              >  2:400582/1‑139 (MQ=255)                                                                                        ttGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATa                              >  2:697064/1‑139 (MQ=255)                                                                                         tGGATCAGATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATaa                             <  1:436360/139‑1 (MQ=255)                                                                                                gATCCGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTc                      >  1:615254/1‑139 (MQ=255)                                                                                                   ccGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAAcc                                          >  2:104783/1‑116 (MQ=255)                                                                                                   ccGCCAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAAcc                                          <  1:104783/116‑1 (MQ=255)                                                                                                      ccAGGCGATGGAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCg                >  1:234979/1‑139 (MQ=255)                                                                                                               ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       >  2:137718/1‑139 (MQ=255)                                                                                                               ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:956564/139‑1 (MQ=255)                                                                                                               ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  2:615254/139‑1 (MQ=255)                                                                                                               ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:596424/139‑1 (MQ=255)                                                                                                               ggAAGAGTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTGTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGaca       <  1:137718/139‑1 (MQ=255)                                                                                                                    agTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAcgc                                                                     >  1:846353/1‑72 (MQ=255)                                                                                                                    agTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGAcgc                                                                     <  2:846353/72‑1 (MQ=255)                                                                                                                    agTAAGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATg  >  1:494215/1‑139 (MQ=255)                                                                                                                        |                                                                                                                                       TCCGGAAAAATGCTGCGCCGCACGATCCAGGCGATTTGCGAAGGACGCGATCCTGGAGATCTGACGACCATTGATGATCCTGCGTCGTTGGATCAGATCCGCCAGGCGATGGAAGAGTAGGTTATTGTCGGATGCGTCGCGCGGTGCATCCGGCACTGTGTGCCGATGCCTGATGCGACGCTGACGCGTTTTATCATGCCTACGGACCTGAACCGTAGGTCGGATAAGGCGCTCGCGTCGCATCCGACACCATG  >  NC_000913/354473‑354726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑