Predicted mutation | ||||||
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evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,921,515 | C→T | T179T (ACG→ACA) | atpB ← | F0 sector of membrane‑bound ATP synthase, subunit a |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,921,515 | 0 | C | T | 97.9% | 145.7 / NA | 47 | T179T (ACG→ACA) | atpB | F0 sector of membrane‑bound ATP synthase, subunit a |
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (19/27); minor base G (0/1); total (19/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCC > NC_000913/3921382‑3921650 | gAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa < 2:551641/139‑1 (MQ=255) gAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCaa < 2:171353/139‑1 (MQ=255) cTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAg > 2:80047/1‑139 (MQ=255) cATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGAt < 2:945507/139‑1 (MQ=255) cATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTACAGTGTCAACTCTTTCGTGAAGCCGCCGAt < 1:187859/139‑1 (MQ=255) aCCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCAtt > 2:509084/1‑139 (MQ=255) aCCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCAtt > 1:975454/1‑139 (MQ=255) aCCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCAtt > 2:725725/1‑139 (MQ=255) ccGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCAttt > 1:230039/1‑139 (MQ=255) aaCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa < 1:509084/139‑1 (MQ=255) aaCAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGa > 1:211812/1‑139 (MQ=255) aaCCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGGACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGaa < 2:1074595/139‑1 (MQ=255) aaCCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGaa < 1:221572/139‑1 (MQ=255) ccGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACa < 1:843655/139‑1 (MQ=255) aGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAAGAGaa > 1:708261/1‑139 (MQ=255) aCAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGcc < 2:688864/139‑1 (MQ=255) agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa < 2:211812/139‑1 (MQ=255) agcagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa < 1:80047/139‑1 (MQ=255) agcagGCTTAACCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGGATCAACTCTTTCGTGAAGCTGCCCATGACCTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa < 2:299412/139‑1 (MQ=255) cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGt > 1:576619/1‑139 (MQ=255) cagGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGt > 2:1025485/1‑139 (MQ=255) ccccTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGa < 1:725725/139‑1 (MQ=255) tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCg > 2:59191/1‑139 (MQ=255) tCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCg > 1:26809/1‑139 (MQ=255) gATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACg < 2:828097/139‑1 (MQ=255) tAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAAt < 2:74284/99‑1 (MQ=255) tAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAAt > 1:74284/1‑99 (MQ=255) aaGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAgg < 2:556698/102‑1 (MQ=255) aaGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAgg > 1:556698/1‑102 (MQ=255) aaGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCa < 2:383732/139‑1 (MQ=255) gACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTcc > 2:368957/1‑139 (MQ=255) cAGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGc < 2:975454/139‑1 (MQ=255) aGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa < 2:50762/107‑1 (MQ=255) aGGAATGAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa > 1:50762/1‑107 (MQ=255) gAACGCCCAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGa < 2:576619/139‑1 (MQ=255) cAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCacac < 1:381639/139‑1 (MQ=255) cAGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCacac < 1:1025485/139‑1 (MQ=255) aGTGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACg < 1:1052010/139‑1 (MQ=255) tGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTgg < 1:659539/64‑2 (MQ=255) tGATTGAACGGCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGt > 2:659539/1‑64 (MQ=255) gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa > 2:313283/1‑81 (MQ=255) gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa < 2:571795/81‑1 (MQ=255) gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa > 1:571795/1‑81 (MQ=255) gCTGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCa < 1:313283/81‑1 (MQ=255) tGCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGAc > 1:652944/1‑139 (MQ=255) gCAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGAcc < 2:561601/139‑1 (MQ=255) cAGTGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGAccc < 2:652944/139‑1 (MQ=255) | GAATGAAAATCAGCTCACCGGCATACATGTTACCGAACAGTCGCAAACCGAGTGAAACTGGTTTGGACAGCAGGCTTACCCCTTCAAGGATTAAGTTGACAGGAATGAACGCCCAGTGATTGAACGGCTGCAGCGTCAACTCTTTCGTGAAGCCGCCGATGCCTTTCATTTTGATGCTGTAGAACAGAATCAGGATAAATACGCCCAGTGCCATAGACAGCGTTACGTTCACGTCCGCAGACGGAACCACACGCAGTGCAGGCAGACCC > NC_000913/3921382‑3921650 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |