Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,937,168	G→A	*297* (TAG→TAA)	rbsB →	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,937,168	0	G	A	100.0%	146.9 / NA	47	*297* (TAG→TAA)	rbsB	D‑ribose ABC transporter periplasmic binding protein, ribose chemotaxis receptor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/25);  total (22/25)

AACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAA  >  NC_000913/3937035‑3937292                                                                                                                                      |                                                                                                                             aaCTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTa                                                                                                                         <  1:412400/139‑1 (MQ=255)      gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                    <  2:528137/139‑1 (MQ=255)      gcagcGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                    >  1:674013/1‑139 (MQ=255)       cagcGACTATCGCTTAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAgg                                                                                                                   <  2:957198/139‑1 (MQ=255)                   cTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                              >  1:198830/1‑132 (MQ=255)                   cTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTa                                                                                                              <  2:198830/132‑1 (MQ=255)                       gCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATgg                                                                                                   <  1:834031/139‑1 (MQ=255)                        cTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCATGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGt                                                                                                  <  2:824483/139‑1 (MQ=255)                            ccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTaa                                                                                                                        <  2:942768/113‑1 (MQ=255)                            ccGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTaa                                                                                                                        >  1:942768/1‑113 (MQ=255)                                  tgagtGGCGCGGAAGGCGGCGAAACCGGAGATAAAGTGCTTAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGACACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATa                                                                                        >  2:591305/5‑139 (MQ=255)                                     ttGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                          <  2:791759/118‑1 (MQ=255)                                     ttGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                          >  1:791759/1‑118 (MQ=255)                                                  cGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        <  2:411796/139‑1 (MQ=255)                                                   gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                          <  2:1062045/104‑1 (MQ=255)                                                   gTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                          >  1:1062045/1‑104 (MQ=255)                                                    tCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                          >  2:113858/1‑103 (MQ=255)                                                    tCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGAc                                                                                                          <  1:113858/103‑1 (MQ=255)                                                                        cTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                    <  1:273038/73‑1 (MQ=255)                                                                        cTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAg                                                                                                                    >  2:273038/1‑73 (MQ=255)                                                                          gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        >  2:662593/1‑115 (MQ=255)                                                                          gAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        <  1:662593/115‑1 (MQ=255)                                                                                  agaAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                        <  2:674013/139‑1 (MQ=255)                                                                                     aaGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCAc                                     <  2:606780/139‑1 (MQ=255)                                                                                             gCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAgg                                                                                                                   <  2:439125/53‑1 (MQ=255)                                                                                             gCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAgg                                                                                                                   >  1:439125/1‑53 (MQ=255)                                                                                             gCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGa                                                                           >  1:1075494/1‑93 (MQ=255)                                                                                             gCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGa                                                                           <  2:1075494/93‑1 (MQ=255)                                                                                                 aGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGa                                                                                                <  1:432195/68‑1 (MQ=255)                                                                                                 aGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGa                                                                                                >  2:432195/1‑68 (MQ=255)                                                                                                       cGGTTGATCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCAt                   >  1:539430/1‑139 (MQ=255)                                                                                                             aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATg                                                                                                    <  2:922014/52‑1 (MQ=255)                                                                                                             aTCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATg                                                                                                    >  1:922014/1‑52 (MQ=255)                                                                                                              tCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        <  2:318888/79‑1 (MQ=255)                                                                                                              tCTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGa                                                                        >  1:318888/1‑79 (MQ=255)                                                                                                               cTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGATCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCa                                      >  2:27503/1‑112 (MQ=255)                                                                                                               cTGAAACTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGATCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCa                                      <  1:27503/112‑1 (MQ=255)                                                                                                                  aaaCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                        >  1:807363/1‑107 (MQ=255)                                                                                                                  aaaCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACgcgc                                        <  2:807363/107‑1 (MQ=255)                                                                                                                    aCTGGTTGTTAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATGAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCATAACACGGTGGCGCATTTTATGGACATcc      >  1:725302/1‑139 (MQ=255)                                                                                                                        gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAata                                                              <  2:385421/79‑1 (MQ=255)                                                                                                                        gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAata                                                              >  1:385421/1‑79 (MQ=255)                                                                                                                        gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcgc                                          >  2:325141/1‑99 (MQ=255)                                                                                                                        gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCAcgc                                          <  1:325141/99‑1 (MQ=255)                                                                                                                        gttgttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGaa  >  1:860428/1‑139 (MQ=255)                                                                                                                            ttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGaa                                                       >  2:607609/1‑82 (MQ=255)                                                                                                                            ttAAGCAGTAATTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGaa                                                       <  1:607609/82‑1 (MQ=255)                                                                                                                                  agtaatTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                   >  2:107036/1‑64 (MQ=255)                                                                                                                                  agtaatTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACgtg                                                                   <  1:107036/64‑1 (MQ=255)                                                                                                                                      |                                                                                                                             AACTAGCAGCGACTATCGCTCAGCTACCCGATCAGATTGGCGCGAAAGGCGTCGAAACCGCAGATAAAGTGCTGAAAGGCGAGAAAGTTCAGGCTAAGTATCCGGTTGATCTGAAACTGGTTGTTAAGCAGTAGTTTTAATCAGGTTGTATGACCTGATGGTGACATAAATACGTCATCGACAGATGAACGTGTAATATAAAGAAAAGCAGGGCACGCGCCACCCTAACACGGTGGCGCATTTTATGGACATCCCGAA  >  NC_000913/3937035‑3937292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑