Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,950,420	G→A	*33* (TAG→TAA)	ilvL →	ilvG operon leader peptide

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,950,420	0	G	A	100.0%	127.3 / NA	40	*33* (TAG→TAA)	ilvL	ilvG operon leader peptide
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/22);  total (18/22)

AGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950286‑3950554                                                                                                                                       |                                                                                                                                       aGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGAt                                                                                                                                    <  2:642836/139‑1 (MQ=255)       tCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGcc                                                                                                                              >  1:558879/1‑139 (MQ=255)        ccTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCt                                                                                                                             >  2:611669/1‑139 (MQ=255)          ttCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTa                                                                                                                           <  1:192870/139‑1 (MQ=255)           tCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTaa                                                                                                                          <  2:544500/139‑1 (MQ=255)                   gATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTaa                                                                                                                  <  2:1076211/139‑1 (MQ=255)                       cAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGAGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAcc                                                                                                              <  2:518400/139‑1 (MQ=255)                                        aCAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGtac                                                                                             >  2:51980/1‑137 (MQ=255)                                           gCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAcccc                                                                                                            <  2:482237/121‑1 (MQ=255)                                           gCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGAcccc                                                                                                            >  1:482237/1‑121 (MQ=255)                                               ttCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCAc                                                                                                       <  1:203482/122‑1 (MQ=255)                                               ttCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCAc                                                                                                       >  2:203482/1‑122 (MQ=255)                                                          aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   >  1:33237/1‑115 (MQ=255)                                                          aTTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   <  2:33237/115‑1 (MQ=255)                                                                         aTTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCga                                                            >  2:803090/1‑139 (MQ=255)                                                                             gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   >  1:783767/1‑96 (MQ=255)                                                                             gCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGaa                                                                                                   <  2:783767/96‑1 (MQ=255)                                                                              cGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTCTGACCTTAAAAACATAACCGAGGAGc                                                       >  2:736164/1‑139 (MQ=255)                                                                               gtggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  1:220763/139‑1 (MQ=255)                                                                               gtggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  1:112043/139‑1 (MQ=255)                                                                                 ggtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  2:558879/139‑1 (MQ=255)                                                                                  gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      >  2:706684/1‑136 (MQ=255)                                                                                  gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  1:899197/136‑1 (MQ=255)                                                                                  gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      <  1:706684/136‑1 (MQ=255)                                                                                  gtggtgATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCa                                                      >  2:899197/1‑136 (MQ=255)                                                                                             tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          <  2:308429/105‑1 (MQ=255)                                                                                             tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCtt                                                                          >  1:308429/1‑105 (MQ=255)                                                                                             tatCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa                                        >  2:332615/1‑139 (MQ=255)                                                                                               tCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc                                      >  1:403757/1‑139 (MQ=255)                                                                                                      gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    <  1:129546/118‑1 (MQ=255)                                                                                                      gTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa                                                    >  2:129546/1‑118 (MQ=255)                                                                                                       tGCGGGGCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAtt                              >  2:398611/1‑139 (MQ=255)                                                                                                            ggCTGCACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt                         <  1:803090/139‑1 (MQ=255)                                                                                                                 cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGttttttt                                                                                 <  2:238031/78‑1 (MQ=255)                                                                                                                 cACTTGGACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGttttttt                                                                                 >  1:238031/1‑78 (MQ=255)                                                                                                                      ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGc                                                                                                         <  1:987873/49‑1 (MQ=255)                                                                                                                      ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGc                                                                                                         >  2:987873/1‑49 (MQ=255)                                                                                                                      ggACGAGGAAAGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt               <  1:332615/139‑1 (MQ=255)                                                                                                                              aaaGGCTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACgg       <  1:611669/139‑1 (MQ=255)                                                                                                                                   cTTAAAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTACGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGaac  <  1:51980/139‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       AGGCATTCCTTCGAACAAGATGCAAGAAAAGACAAAATGACAGCCCTTCTACGAGTGATTAGCCTGGTCGTGATTAGCGTGGTGGTGATTATTATCCCACCGTGCGGGGCTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATTCAGGACGGGGAAC  >  NC_000913/3950286‑3950554

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑