Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,984,193	G→A	*412* (TAG→TAA)	aslB →	putative AslA‑specific sulfatase‑maturating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,984,193	0	G	A	100.0%	117.3 / NA	37	*412* (TAG→TAA)	aslB	putative AslA‑specific sulfatase‑maturating enzyme
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/17);  total (20/17)

ATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAG  >  NC_000913/3984063‑3984327                                                                                                                                   |                                                                                                                                       attTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATcggc                                                                                                                                >  2:421792/1‑139 (MQ=255)       gtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgccgc                                                                                                                          >  2:583498/1‑139 (MQ=255)       gtgCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGgccgc                                                                                                                          >  2:578197/1‑139 (MQ=255)                               gCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATgcggc                                                                                                  >  2:28876/1‑139 (MQ=255)                                             tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                    <  1:583498/139‑1 (MQ=255)                                             tatCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGCGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                    <  1:639354/139‑1 (MQ=255)                                             tatCTTAAAGAAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATc                                                                                    <  2:794286/139‑1 (MQ=255)                                               tCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  2:719766/139‑1 (MQ=255)                                               tCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  1:28876/139‑1 (MQ=255)                                               tCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  1:958791/139‑1 (MQ=255)                                                    aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  1:857458/134‑1 (MQ=255)                                                    aaGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  >  2:857458/1‑134 (MQ=255)                                                         aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                        >  1:63757/1‑139 (MQ=255)                                                         aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                        >  1:798475/1‑139 (MQ=255)                                                         aTGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                        >  1:515957/1‑139 (MQ=255)                                                          tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  1:1012360/128‑1 (MQ=255)                                                          tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  >  1:416308/1‑128 (MQ=255)                                                          tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  >  2:1012360/1‑128 (MQ=255)                                                          tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  2:416308/128‑1 (MQ=255)                                                          tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                        <  1:75630/138‑1 (MQ=255)                                                          tGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGa                                                                        >  2:75630/1‑138 (MQ=255)                                                               gATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGc                                                                  <  2:515957/139‑1 (MQ=255)                                                                     cTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTc                                                            >  1:587424/1‑139 (MQ=255)                                                                      tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  >  1:621753/1‑116 (MQ=255)                                                                      tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  2:621753/116‑1 (MQ=255)                                                                      tGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCa                                                           <  2:798475/139‑1 (MQ=255)                                                                                         cAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGt                                        <  1:625527/139‑1 (MQ=255)                                                                                                  tATGCATGCGCATTTGCTGGTGGTGAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTggct                               >  1:314036/1‑137 (MQ=255)                                                                                                                     gtggtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATa                              >  2:282323/1‑121 (MQ=255)                                                                                                                     gtggtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATa                              <  1:282323/121‑1 (MQ=255)                                                                                                                       ggtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatcgcatc          >  2:554219/1‑139 (MQ=255)                                                                                                                       ggtgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAgcatcgcatc          >  1:208968/1‑139 (MQ=255)                                                                                                                         tgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                             >  2:96732/1‑70 (MQ=255)                                                                                                                         tgAGTAAGTAAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                                             <  1:96732/70‑1 (MQ=255)                                                                                                                               agtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  >  2:851827/1‑59 (MQ=255)                                                                                                                               agtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCg                                                                                  <  1:851827/59‑1 (MQ=255)                                                                                                                               agtaAAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAg  >  2:700748/1‑139 (MQ=255)                                                                                                                                   |                                                                                                                                       ATTTGTGTGCCGGGTATCAGCGTTATTTCCGCCATCTACCGCCATATCTTAAAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAGTAAGTAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAG  >  NC_000913/3984063‑3984327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑