Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,986,686	C→T	*399* (TAG→TAA)	hemY ←	putative protoheme IX synthesis protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,986,686	0	C	T	100.0%	159.7 / NA	50	*399* (TAG→TAA)	hemY	putative protoheme IX synthesis protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (29/21);  total (29/21)

ATGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGT  >  NC_000913/3986552‑3986812                                                                                                                                       |                                                                                                                               aTGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAattact                                                                                                                            >  2:698847/1‑139 (MQ=255)  tGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACtg                                                                                                                           >  2:1001563/1‑139 (MQ=255)          aCGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGt                                                                                                                   <  1:1001563/139‑1 (MQ=255)              tGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTAtt                                                                                                               >  2:555254/1‑139 (MQ=255)                     aGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAAc                                                                                                        >  1:302567/1‑139 (MQ=255)                                    tCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCg                                                                                         <  1:555254/139‑1 (MQ=255)                                     cATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGt                                                                                        >  1:376134/1‑139 (MQ=255)                                            aCCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACg                                                                                 >  2:274360/1‑139 (MQ=255)                                            aCCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACg                                                                                 <  1:585033/139‑1 (MQ=255)                                                      aCGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCa                                                                       >  1:785069/1‑139 (MQ=255)                                                       cGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAg                                                                      <  2:823266/139‑1 (MQ=255)                                                        gCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCg                                                                                                                      >  2:528305/1‑90 (MQ=255)                                                        gCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCg                                                                                                                      <  1:528305/90‑1 (MQ=255)                                                             cTCCGTAATAACAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGcttctt                                                                <  1:698847/139‑1 (MQ=255)                                                                       agagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACtg                                                                                                                           >  2:454363/1‑70 (MQ=255)                                                                       agagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACtg                                                                                                                           <  1:454363/70‑1 (MQ=255)                                                                        gagCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGc                                                     <  2:472540/139‑1 (MQ=255)                                                                          gCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCa                                                                                                          >  2:132542/1‑84 (MQ=255)                                                                          gCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCa                                                                                                          <  1:132542/84‑1 (MQ=255)                                                                          gCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAg                                                   >  1:948640/1‑139 (MQ=255)                                                                            aGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcg                                                                                      >  1:886754/1‑102 (MQ=255)                                                                            aGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcg                                                                                      <  2:886754/102‑1 (MQ=255)                                                                            aGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTc                                                 >  2:274424/1‑139 (MQ=255)                                                                             ggCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCt                                                                                                             <  2:219144/78‑1 (MQ=255)                                                                             ggCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCt                                                                                                             >  1:219144/1‑78 (MQ=255)                                                                             ggCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAg                                                   <  1:903221/136‑1 (MQ=255)                                                                             ggCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAg                                                   >  2:903221/1‑136 (MQ=255)                                                                             ggCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCt                                                >  2:26969/1‑139 (MQ=255)                                                                             ggCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCt                                                >  1:587980/1‑139 (MQ=255)                                                                              gCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTg                                               >  1:737332/1‑139 (MQ=255)                                                                               cGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCg                                                                                                                      >  2:170618/1‑67 (MQ=255)                                                                               cGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCg                                                                                                                      <  1:170618/67‑1 (MQ=255)                                                                                           tATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCgg                                  <  2:874772/139‑1 (MQ=255)                                                                                             tCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcgcg                                                                                    >  2:985606/1‑87 (MQ=255)                                                                                             tCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTcgcg                                                                                    <  1:985606/87‑1 (MQ=255)                                                                                              cAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCaa                               >  2:390131/1‑139 (MQ=255)                                                                                                    ggCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATg                         >  2:562420/1‑139 (MQ=255)                                                                                                     gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCAt                                                                              <  1:935731/85‑1 (MQ=255)                                                                                                     gCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCAt                                                                              >  2:935731/1‑85 (MQ=255)                                                                                                      ccccGGAGGTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCg                       <  2:376134/139‑1 (MQ=255)                                                                                                           gAGGTGCTTGCCTCCGGGTGAGAAGGAATTACtgtg                                                                                                                         <  1:845450/36‑1 (MQ=255)                                                                                                           gAGGTGCTTGCCTCCGGGTGAGAAGGAATTACtgtg                                                                                                                         >  2:845450/1‑36 (MQ=255)                                                                                                              gTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTa                                                                                                                 <  1:743169/41‑1 (MQ=255)                                                                                                              gTGCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTa                                                                                                                 >  2:743169/1‑41 (MQ=255)                                                                                                                gCTTGCCTCCGGGTGAGAAGGAATTACTTTGGCGGGTTATTCTGCa                                                                                                          >  1:666310/1‑46 (MQ=255)                                                                                                                gCTTGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCa                                                                                                          <  2:666310/46‑1 (MQ=255)                                                                                                                   tGCCTCCGGGTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGt          >  2:35671/1‑139 (MQ=255)                                                                                                                     cgccacggTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACg                                                                                 <  1:632895/60‑1 (MQ=255)                                                                                                                     cgccacggTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACg                                                                                 >  2:632895/7‑66 (MQ=255)                                                                                                                      ctctgtgTGAAAAGGAATGACTTGGGCGGGTTATTCTGCAACGTTAATATCAACCCGGCGTTACGCATAGCTGCAGCTTCTTACGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCg       <  2:930643/133‑1 (MQ=255)                                                                                                                           ggTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACgt  >  2:772200/1‑139 (MQ=255)                                                                                                                           ggTGAGAAGGAATTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACgt  >  2:650049/1‑139 (MQ=255)                                                                                                                                       |                                                                                                                               ATGGACACAACGTTGAGTGAAGCACCCACTTGTTGTCATACAGACCTGTTTTAACGCCTGCTCCGTAATAAGAGCAGGCGTTTTTTTATGTATCAGGAAGGCCCCGGAGGTGCTTGCCTCCGGGTGAGAAGGAACTACTGTGGCGGGTTATTCTGCAACGTTAACATCAAACCGTCGCGACGCATAGCTGCAGCTTCTTCCGGCTTGTGCAGTCTGTCCAGCGCGTCGGCAAGCCATGCGTAATCGTAGGCGTCCGGACGT  >  NC_000913/3986552‑3986812

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑