Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,024,336	G→A	*261* (TAG→TAA)  L162L (CTC→CTT)	tatD → rfaH ←	quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase transcription antitermination protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,024,336	0	G	A	100.0%	228.8 / NA	71	*261* (TAG→TAA)  L162L (CTC→CTT)	tatD rfaH	quality control of Tat‑exported FeS proteins, Mg‑dependent cytoplasmic DNase transcription antitermination protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/37);  total (34/37)

CATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGTAA  >  NC_000913/4024200‑4024469                                                                                                                                         |                                                                                                                                      catcatCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTaaag                                                                                                                                     >  1:571370/1‑139 (MQ=255)                 cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      >  2:528721/1‑138 (MQ=255)                 cGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      <  1:528721/138‑1 (MQ=255)                      cagccCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                                >  1:82329/1‑139 (MQ=255)                          ccATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATAGCGCATGGCTGGCTGCCACCACGGATGATAATGTCAAAACACTGTTTGGGATAGCGTTTTAAAGTTTCCGGAACTCGGTATTCTTcaca                                                                                                            <  2:647269/139‑1 (MQ=255)                           cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           <  2:559080/139‑1 (MQ=255)                           cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           <  1:862895/139‑1 (MQ=255)                           cATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCGGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           <  2:853300/139‑1 (MQ=255)                              cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGGGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                        >  1:1013551/1‑139 (MQ=255)                              cTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACtgt                                                                                                        >  2:77519/1‑139 (MQ=255)                                     atatTTTGCAACGTATTGCGCACTGGCGTGGGGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAAt                                                                                                 <  2:1013551/139‑1 (MQ=255)                                         tttGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATctct                                                                                             <  2:697998/139‑1 (MQ=255)                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACt                                                                                                                          <  1:693737/108‑1 (MQ=255)                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACt                                                                                                                          >  2:693737/1‑108 (MQ=255)                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      >  1:282776/1‑112 (MQ=255)                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      <  1:256658/112‑1 (MQ=255)                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      >  2:256658/1‑112 (MQ=255)                                           tGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      <  2:282776/112‑1 (MQ=255)                                                  tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                        <  1:912775/103‑1 (MQ=255)                                                  tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCg                                                                                                                        >  2:912775/1‑103 (MQ=255)                                                  tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      >  1:738324/1‑105 (MQ=255)                                                  tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      <  2:738324/105‑1 (MQ=255)                                                  tATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaata                                                                                    >  2:240049/1‑139 (MQ=255)                                                  tATTGCGAACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaata                                                                                    >  2:529885/1‑139 (MQ=255)                                                    ttGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAg                                                                                  >  2:508703/1‑139 (MQ=255)                                                     tGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGa                                                                                 <  2:21765/139‑1 (MQ=255)                                                          aCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaataa                                                                                   >  2:81412/1‑132 (MQ=255)                                                          aCTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaataa                                                                                   <  1:81412/132‑1 (MQ=255)                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCa                                                                          <  1:240049/139‑1 (MQ=255)                                                            tGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGG‑ACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                         >  1:480848/1‑139 (MQ=255)                                                             ggCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCaa                                                                         <  2:571370/139‑1 (MQ=255)                                                              gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      >  1:187653/1‑93 (MQ=255)                                                              gCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      <  2:187653/93‑1 (MQ=255)                                                               cGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                    >  1:1049631/1‑94 (MQ=255)                                                               cGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAt                                                                                                                    <  2:1049631/94‑1 (MQ=255)                                                                 tGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGG‑ACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                    <  2:480848/139‑1 (MQ=255)                                                                  tgataagaTGCCGCATGGCTGGCTGCCACCACGGATGCGAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTTTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCa                                                                    <  2:488324/135‑1 (MQ=255)                                                                    agaagaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATg                                                                  >  1:923941/1‑139 (MQ=255)                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttct                                                                                                                 >  1:25354/1‑88 (MQ=255)                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttct                                                                                                                 <  2:25354/88‑1 (MQ=255)                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                            >  2:57307/1‑93 (MQ=255)                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTcaca                                                                                                            <  1:57307/93‑1 (MQ=255)                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagc                                                              >  1:143112/1‑139 (MQ=255)                                                                        gaTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagc                                                              >  2:816167/1‑139 (MQ=255)                                                                         aTGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgagcg                                                             <  1:816167/139‑1 (MQ=255)                                                                              gCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCt                                                        <  2:923941/139‑1 (MQ=255)                                                                                aTGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCa                                                      <  2:209059/139‑1 (MQ=255)                                                                                 tggctggcTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCg                                                                                                                               >  2:346428/1‑65 (MQ=255)                                                                                 tggctggcTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCg                                                                                                                               <  1:346428/65‑1 (MQ=255)                                                                                        ctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      >  1:147497/1‑67 (MQ=255)                                                                                        ctgCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGt                                                                                                                      <  2:147497/67‑1 (MQ=255)                                                                                          gCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGtt                                            <  1:508703/139‑1 (MQ=255)                                                                                           ccaccaCGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTc                                           >  2:359663/1‑139 (MQ=255)                                                                                                                       gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                                >  1:895601/1‑42 (MQ=255)                                                                                                                       gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTAttctt                                                                                                                <  2:895601/42‑1 (MQ=255)                                                                                                                       gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                               >  1:1049382/1‑91 (MQ=255)                                                                                                                       gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGgag                                                               <  2:1049382/91‑1 (MQ=255)                                                                                                                       gTTTGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAc               <  1:504451/139‑1 (MQ=255)                                                                                                                         ttGGGATTGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAcgc             <  1:529885/139‑1 (MQ=255)                                                                                                                                tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           <  2:333998/38‑1 (MQ=255)                                                                                                                                tGCGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           >  1:333998/1‑38 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           >  1:985613/1‑36 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCacac                                                                                                           <  2:985613/36‑1 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                   <  1:975698/44‑1 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTa                                                                                                   >  2:975698/1‑44 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaataa                                                                                   >  1:892970/1‑60 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATtaataa                                                                                   <  2:892970/60‑1 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAcgcg            <  2:600723/131‑1 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAAcgcg            >  1:600723/1‑131 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt    >  2:439665/1‑139 (MQ=255)                                                                                                                                  cGTTTTAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGt    >  2:208185/1‑139 (MQ=255)                                                                                                                                    ttttAAAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGtaa  >  2:901632/1‑139 (MQ=255)                                                                                                                                         |                                                                                                                                      CATCATCCCGGCGCAACGAGCCAGCCCATCTGCCCCATATTTTGCAACGTATTGCGCACTGGCGTGGAGAAGATGCCGCATGGCTGGCTGCCACCACGGATGCTAATGTCAAAACACTGTTTGGGATTGCGTTTTAGAGTTTGCGGAACTCGGTATTCTTCACACTGTGCTTAATCTCTTTATTAATAAGATTAAGCAATAGCATGGAGCGAGCCTCACCATCGGGTTCGGTGAAAATGGCCTGAAAGCCTTCGAACGCGCCTTCGGTAA  >  NC_000913/4024200‑4024469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑