Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,075,454	G→A	*262* (TAG→TAA)	yihW →	putative transcriptional regulator for sulphoquinovose utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,075,454	0	G	A	100.0%	109.7 / NA	36	*262* (TAG→TAA)	yihW	putative transcriptional regulator for sulphoquinovose utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/23);  total (13/23)

ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTT  >  NC_000913/4075318‑4075572                                                                                                                                         |                                                                                                                       aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                      >  1:204573/1‑139 (MQ=255) aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                      >  1:509928/1‑139 (MQ=255) aTGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAAt                                                                                                                      >  1:982426/1‑139 (MQ=255)      tCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtg                                                                                                                 <  2:395041/139‑1 (MQ=255)       cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                <  2:204573/139‑1 (MQ=255)       cggcggcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTtgt                                                                                                                <  1:692773/139‑1 (MQ=255)            gcggTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCg                                                                                                           <  1:427109/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCTAAATTGAAATCATTCTTCCCCAGTAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     <  1:184877/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     <  2:982426/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     <  1:464562/139‑1 (MQ=255)                  gAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     <  1:946436/139‑1 (MQ=255)                    aaTTGGTAATGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTg                                                                                                   <  2:633616/139‑1 (MQ=255)                            aCGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATa                                                                                           <  2:1052060/139‑1 (MQ=255)                                           cTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGcc                                                                            <  2:509928/139‑1 (MQ=255)                                                      aCCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAACCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                 <  1:15815/123‑1 (MQ=255)                                                      aCCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAACCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCcaca                                                                                 >  2:15815/1‑123 (MQ=255)                                                                    gcccgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     >  2:254770/1‑89 (MQ=255)                                                                    gcccgcTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     <  1:254770/89‑1 (MQ=255)                                                                              cTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTtgt                                         <  2:503358/139‑1 (MQ=255)                                                                                      aCGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     <  1:242257/71‑1 (MQ=255)                                                                                      aCGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAAcc                                                                                                     >  2:242257/1‑71 (MQ=255)                                                                                                     ccAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGc                  <  2:356586/139‑1 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                        >  1:460853/1‑100 (MQ=255)                                                                                                      cAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTa                                                        <  2:460853/100‑1 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                      <  1:221646/132‑1 (MQ=255)                                                                                                        aaTTGAAATCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaa                      >  2:221646/1‑132 (MQ=255)                                                                                                               aTCATTCTTCCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCtt        >  1:104657/1‑139 (MQ=255)                                                                                                                     cttcCCCAGGAAGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGAttt  <  2:104657/139‑1 (MQ=255)                                                                                                                                aGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGct                 >  1:893099/1‑113 (MQ=255)                                                                                                                                aGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGct                 <  2:893099/113‑1 (MQ=255)                                                                                                                                aGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat             <  2:959917/117‑1 (MQ=255)                                                                                                                                aGACGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCtat             >  1:959917/1‑117 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATa                                                                                           >  2:338036/1‑37 (MQ=255)                                                                                                                                  aCGCGTAAATTTTTGTGACCGTTAACCTGGCTTCATa                                                                                           <  1:338036/37‑1 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaaa                     >  1:818701/1‑106 (MQ=255)                                                                                                                                   cgcgTAAATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCaaaaa                     <  2:818701/106‑1 (MQ=255)                                                                                                                                         |                                                                                                                       ATGCTTCGGCGGCGGTTGAAATTGGTAACGTGGCACAGGTCACTGCGCTCTTTACCGACGAGCTGCCGCCCGCTGCGCTAAAATCACGCTTACAAGACAGCCAAATTGAAATCATTCTTCCCCAGGAAGACGCGTAGATTTTTGTGACCGTTAACCTGGCTTCATACCTTGCCACATAGCCAAACCCATCCTTTCCCGCTACAGTTAATTTCTTGTGGCGCGAAAGGAGGCAAAAATGCTCTATATCTTTGATTT  >  NC_000913/4075318‑4075572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑