Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,094,673	G→A	intergenic (‑401/+50)	rhaD ← / ← rhaA	rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,094,673	0	G	A	100.0%	63.0 / NA	22	intergenic (‑401/+50)	rhaD/rhaA	rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/11);  total (11/11)

GATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGG  >  NC_000913/4094600‑4094807                                                                          |                                                                                                                                       gATAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTc                                                                       >  2:40301/1‑139 (MQ=17)   tAAGGCGCTCGCGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCaa                                                                     >  2:755955/1‑139 (MQ=17)         gctcgcgCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttc                                                               <  1:969528/133‑1 (MQ=255)           tcgcgCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAAtttctt                                                             <  1:790405/135‑1 (MQ=255)                                       aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCgg                                                                                             >  1:359283/1‑79 (MQ=255)                                       aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCgg                                                                                             <  2:359283/79‑1 (MQ=255)                                       aTGCGACGCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCa                                 >  1:582651/1‑139 (MQ=255)                                              gCTGTCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTa                          <  1:755955/139‑1 (MQ=255)                                                  tCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAg                                    <  1:184015/125‑1 (MQ=255)                                                  tCGCGTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAg                                    >  2:184015/1‑125 (MQ=255)                                                    gcgTCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACctgct                    <  1:40301/139‑1 (MQ=255)                                                       tCTTATCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGc                 >  2:68890/1‑139 (MQ=255)                                                            tCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATc            >  1:1024639/1‑139 (MQ=255)                                                            tCAGGCCTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATc            >  2:194322/1‑139 (MQ=255)                                                                 ccTACAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGAc       <  2:490182/139‑1 (MQ=255)                                                                    aCAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCa                                              >  1:830136/1‑97 (MQ=255)                                                                    aCAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCa                                              <  2:830136/97‑1 (MQ=255)                                                                    aCAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACg      <  2:1037514/137‑1 (MQ=255)                                                                    aCAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACg      >  1:1037514/1‑137 (MQ=255)                                                                     cAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCt                           <  2:498727/115‑1 (MQ=255)                                                                     cAACTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCt                           >  1:498727/1‑115 (MQ=255)                                                                      aaCTATTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTgg  <  2:582651/139‑1 (MQ=255)                                                                          |                                                                                                                                       GATAAGGCACTTGTGCCGCATCCGGCAATCAATGCCTGATGCGACGCTGTCGCGTCTTATCAGGCCTACAACTGTTGCCGACCCGTAGGCCGGATAAGGCGCTCGCGCCGCATCCGGCAGTGTTTACCCGCGGCGACTCAAAATTTCTTTCTCATAAGCCCGCACGCTCTCCAGCCATTCGCTACCTGCTGGCGTATCGTGACGTTGG  >  NC_000913/4094600‑4094807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑